EFFECTS OF CHRONIC LEAD INTAKE VIA DRINKING WATER ON SOME HEMATOLOGIC PARAMETERS IN MICE

Öz

The aim of this study was to investigate the effects of chronic lead intake by drinking water at the dosages of 250 and 1000 ppm of lead (as lead acetate) on some hematologic parameter in albino mice. A total of 120 six-month-old 120 white male albino mice, weighing 35- 40 g fed with same pellet feed and water were used in this study. Animals were equally divided into three groups of 40 animals each. Group I served as a control. Group II and group III received 250 ppm and 1000 ppm lead acetate via drinking water during 120 days, respectively. Blood samples were taken by cardiac puncture on days 60, 90 and 120 of study from animals. On days 60 and 90 there were no statistical difference in hematological parameters between groups. On day 120, mice in group III received 1000 ppm lead ecetate had significantly lower erythrocyte count, hematocrit and hemoglobin, MCV and MCH values than the control mice. Additionally, platelet counts, total leukocyte counts and lymphocyte ratios were significantly lower in lead-treated mice than the control animals. On the other hand, neutrophil ratio in lead treated mice were significantly elevated compared with the control mice. In conclusion, it was observed that lead acetate at the dose of 250 ppm does not affect the hematologic parameters while lead acetate at the dose of 1000 ppm leads to hematological disorders.

Anahtar Kelimeler

• Mouse, chronic lead intake, hematologic parameter

Farelerde İçme Suyu ile Kronik Kurşun Alımının Bazı Hematolojik Parametreler Üzerine Etkisi

Öz

Bu çalışma, 250 ve 1000 ppm dozunda kurşun asetat içeren içme sularının farelere kronik uygulanmasının bazı hematolojik parametrelerelere etkisini incelemek amacıyla yapılmıştır. Çalışmada 6 aylık, 35-40 g ağırlığında 120 erkek beyaz fare kullanıldı. Hayvanlar her bir grupta 40 fare olacak şekilde üç eşit gruba ayrıldı. I.grup control grubu olarak tutulurken II. ve III. gruptaki farelere 250 ve 1000 ppm kurşun asetat içeren su 120 gün süreyle verildi. Hayvanlardan 60., 90. ve 120. günlerde kalpten kan örnekleri alınarak incelendi. İncelenen hematolojik parametrelerde 60. ve 90. günlerde her hangi bir farklılık belirlenemedi. 1000 ppm dozda kurşun alan III. gruptaki hayvanların kontrol gurubuna göre eritrosit, lökosit, platelet sayısı ve lenfosit oranı ile ile hemoglobin, hematokrit, MCV ve MCH değerlerinde önemli bir azalma gözlenirken nötrofil oranında ise önemli bir artma tespit edilmiştir. Sonuç olarak, 250 ppm dozda kronik olarak kurşun alımının hematalojik parametreleri etkilemediği buna karşılık 1000 ppm dozda kurşun asetatın alımının ise hematolojik bozukluklara yol açtığı gözlenmiştir

Anahtar Kelimeler

• Fare, kronik kurşun alımı, hematolojik parametre

Kaynakça

1. 1. Rampley CG, Ogden KL. Preliminary studies for removal of lead from surrogate and real soils using a water soluble chelator. Environ Sci Tech 1998, 32: 987-993.
2. 2. Palacios H, Iribarren I, Olalla MJ, Cala V. Lead poisoning of horses in the vicinity of a battery recycling plant. Sci Total Environ 2002, 290: 81-89.
3. 3. Harkness JE, Wagner JE. The biology and medicine of rabbits and rodents. Fourth edition, Williams & Wilkins, Baltimore 1995, pp: 93.
4. 4. Jacob B, Ritz B, Heinrich J, Hoelscher B, Wichman E. The effect of low-l level blood lead on hematologic parameters in children. Environ. Res Sec A 2000, 82:150-159.
5. 5. Othman AI, Sharawy AI, Missry MA. Role of melatonin in ameliorating lead induced haematotoxicity. Phar Res 2004, 50: 301-307.
6. 6. Liu ZP. Lead poisoning combined with cadmium in sheep and horses in the vicinity of non-ferrous metal smelters. Sci Environ 2003, 309: 117-126.
7. 7. Harada K, Miura H. Heme synthesis and iron turnover in rabbits with experimental lead poisoning. Sangyo Igaku 1983, 25: 161-174.
8. 8. Altınsaat Ç, Uzun M, Sulu N, Öztürkmen A. Kobaylarda kurşun asetat uygulamasının bazı hematologic değerler üzerine etkisi. Ankara Üniv Vet Fak Derg 1997, 44:249-258.

9. 9. Moore M R. Hematological effects of lead. Sci Environ 1988, 71: 419-431.
10. 10. Donaldson WE, Knowles SO. Is lead toxicosis a reflection of altered fatty acis composition of membranes Comp Biochem Physiol C 1993, 104: 377-379.
11. 11. De Silva PE. Determination of lead in plasma and studies on its relationship to lead in erythrocytes. Br J Ind Med 1981, 38: 209-217.
12. 12. Rice-Evans. Iron-mediated oxidative stress and erythrocytes. In: J R Harris( Ed.) Blood cell biochemistry (Vol. I), Plenum Press, New York, 1990 pp 429- 453.
13. 13. Levander OA, Morris VC, Ferretti RJ. Filterability of erythrocytes from vitamin Edeficient lead-poisoned rats. J Nutr 1977, 107: 363-372.
14. 14. Yoshida K, Sakurai H, Toyama T. Immunological parameters in lead workers. Sangyo Igaku 1980, 22: 488-93.
15. 15. Van Den Heuvel RL, Leppens H, Schoeters GE. Lead and catechol hematoxicity in vitro using human and murine hematopoietic progenitor cells. Cell Biology and Toxicology 1999, 15: 101-110.
16. 16. Peng S, Zhang C, Wang C, et al. The investigation on the changes of hematological parameters in the occupationally lead exposed workers. Zhonghau Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2002, 20: 334.