FAREDE AKUT AĞRI MODELİNDE SERTRALİNİN OLUŞTURDUĞU ANTİNOSİSEPTİF CEVABA KAFEİNİN ETKİSİ

Öz

Antidepresan ilaçlar kronik ağrı durumlarının tedavisinde oldukça yaygın kullanılırlar. Bir antidepresan ilaç olan sertralinin de deneysel ve klinik çalışmalarda çeşitli ağrı durumlarında etkili olduğu gösterilmiştir. Ancak sıkça tüketilen bir alkaloid olan ve bazı antidepresanların antinosiseptif etkilerini değiştirdiği gösterilen kafeinin, sertralinin oluşturduğu antinosisepsiyon üzerine herhangi bir etkisinin olup olmadığına yönelik bir bilgi bulunmamaktadır. Bu nedenle; sunulan çalışmada akut olarak farelere uygulanan sertralinin oluşturduğu antinosisieptif etki üzerine kafeinin olası rolünün araştırılması amaçlanmıştır. Çalışmada 32 adet erkek ve dişi Swiss albino fare; SF+SF, kafein (5 mg/kg)+SF, SF+sertralin (40 mg/kg) ve kafein (5 mg/kg)+sertralin (40 mg/kg) injeksiyonu uygulanan dört gruba ayrılıp uygulanan drog kombinasyonlarının etkisi hot-plate analjezimetresi ile test edildi. Sertralinin 30., 45. dakikalarda antinosiseptif etki gösterdiği ve bu etkinin 60. dakikada azaldığı, kafeinin ise tek başına herhangi bir antinosiseptif etki göstermediği, sertralin ve kafein kombine edildiğinde sertralinin antinosiseptif etkisinde istatistiksel olarak anlamlı bir değişiklik meydana gelmediği belirlendi. Sonuç olarak farede sertralinin akut uygulama ile oluşturduğu antinosiseptif cevabın uygulanan dozda kafein ile değişmediği gösterildi

Anahtar Kelimeler

• Sertralin, kafein, antinosisepsiyon, fare

Effect of Caffeine on Antinociceptive Response of Sertraline in Acute Pain Model in Mice

Öz

Antidepressant drugs are widely used in the treatment of chronic pain states. The effectiveness of sertraline, an antidepressant drug, has been shown in different pain states in experimental and clinical studies. However, there is no information whether caffeine, a widely consumed alkaloid known to change the antinociceptive effects of some antidepressant drugs, has an effect on sertraline induced antinociception. Therefore, in the present study, we aimed to investigate the possible role of caffeine on sertraline induced acute antinociceptive response in mice. In the study, 32 male and female Swiss albino mice were separated into four groups (n=8) and injected with saline+saline, caffeine (5mg/kg)+saline, saline+sertraline (40mg/kg) and caffeine (5mg/kg)+sertraline (40mg/kg). The effects of these drug combinations were tested with a hot-plate analgesiameter. It was found that sertraline induced an antinociceptive effect at the 30th and 45th minutes, and the effect decreased at the 60th minute, whereas caffeine alone did not induce an antinociceptive response. When sertraline was combined with caffeine, the antinociceptive effect of sertraline did not change significantly. As a result, it was deduced that the antinociceptive response of sertraline in acute application in mice was not affectedby an applied dose of caffeine

Anahtar Kelimeler

• Sertraline, caffeine, antinociception, mice

Kaynakça

1. Sawynok J, Eser MJ, Reid AR. Antidepressants as analgesics: an overview of central and peripheral mechanisms of action. J Psychiatry Neurosci 2001, 26 (1):21-29.
2. Giannopoulos S, Kosmidou M, Sarmas I, et al. Patient compliance with SSRIs and gabapentin in painful diabetic neuropathy. Clin J Pain 2007, 23:267-269.
3. Schreiber S, Pick CG. From selective to highly selective SSRIs: A comparison of the antinociceptive properties of fluoxetine, fluvoxamine, citalopram and escitalopram. Eur Neuropsychopharmacol 2006, 16:464- 468. 4. Goodnick PJ, Jimenez I, Kumar A. Sertraline treatment of diabetic neuropathy. VI. World Congress of Biological Psychiatry, Nice France, 22-27 June 1997 Biol Psychiatry 1997, 42(1):238.
4. Varia I, Logue E, O’Connor C. et al. Randomized trial of sertraline in patients with unexplained chest pain of noncardiac origin. American Heart Journal September 2000, 140(3):367-372 .
5. Siva A. Nörolojide akılcı ilaç kullanımı. Akılcı ilaç kullanımı sempozyumu bildiri kitabı, İstanbul 14 Ocak 1999, ss. 117-141. 7. Lee RA, West RM, Wilson JD. The response to sertraline in men with chronic pelvic pain syndrome. Sex Transm Infect 2005, 81:147- 149. 8. Doksat MK. Dünyası 1999, 1:23-31.
6. Rybicka IK, Plaznik A. Analgesic effect of antidepressant drugs. Pharmacol Biochem Behav 1998, 59(2):331-338.
7. Mattia C, Paoletti F, Coluzzi F, Boanelli A. New antidepressants in the treatment of neuropathic pain. Minerva Anestesiol 2002, 68:105-114.
8. Sawynok J. Adenosine receptor activation and nociception. Eur J Pharmacol 1998, 317:1-11.

9. Esser MJ, Sawynok J. Caffeine blockade of the thermal antihyperalgesic effect of acute amitriptyline in a rat model of neuropathic pain. Eur J Pharmacol 2000, 399:131-139.
10. Paton C, Beer D. Caffeine: The forgotten variable. International Journal of Psychiatry in Clinical Practice 2001, 5:231-236.
11. Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain 1983, 16:109-110.
12. Fürst S. Transmitters involved in antinociception in the spinal cord. Brain Res Bull 1999, 48(2):129-141.
13. Phillis JW, Wu PH. The effect of various centrally active drugs on adenosine uptake by the central nervous system. Com Biochem Physiol 1982, 72:179-187.
14. Pareek SS, Chopde CT, Thakur DPA. Adenosine enhances anlagesic effect of tricyclic antidepressants. Indian J Pharmacol 1994, 26:159-161.
15. Yaba G, Sezer Z, Tekol Y. Interaction between venlafaxine and caffeine on antinociception in mice. Pharmazie 2006, 61 (1):60-62.
16. Redrobe JP, Bourin M. Dose-dependent influence of buspirone on the activities of selective serotonin reuptake inhibitors in the mouse forced swimming test Psychopharmacol 1998, 138:198-206.
17. Sawynok J, Eser MJ, Reid AR. Peripheral antinociceptive actions of desipramine and fluoxetine in an inflammatory and neuropathic pain test in the rat. Pain 1999, 82:149-158.
18. Soto VG, Hernandez GC. A review of the pharmacokinetic and pharmacodynamic factors in the potentiation of the antinociceptive effect of nonsteroidal anti- inflammatory drugs by caffeine. J Pharmacol Toxicol 1999, 42:67-72.
19. Lopez JRM, Ramirez AMD, Cook HJ, et al. Enhancement of antinociception by co- administration of ibuprofen and caffeine in arthritic rats. Eur J Pharmacol 2006, 544:31-38.
20. Wladyslawa AD, Kot M, Wojcikowski J. Effects of classic and newer antidepressants on the oxidation pathways of caffeine in rat liver in vitro study. Pol J Pharmacol 2003, 55:1045-1053.
21. Hariri AG, Ceylan E. Depresyonda ilaç tedavisi: Özgül serotonin geri alım engelleyicileri. Duygudurum dizisi 2003, 7:339-345.