RATLARDA TİYOSEMİKARBAZON TÜREVLERİNİN BAZI KAN PARAMETRELERİNE ETKİLERİ

Öz

Çalışmada, deney grubu ratlara 45 günlük deneysüresince üçer gün ara ile deri altı yolla 25 mg/kgtiyosemikarbazon ve 25 mg/kg tiyosemikarbazon-çinkokompleksi uygulandı. Uygulamaların serum aspartatamino transferaz (AST), alanin amino transferaz (ALT),laktat dehidrogenaz (LDH), gamma-glutamil transferaz(GGT) aktivitelerine ve üre, kreatinin, total ve direktbilirubin düzeylerine, karaciğer ağırlığına ve canlıağırlığına etkileri belirlemek için 60 adet rat kullanıldı.Ratlar kontrol grubu, tiyosemikarbazon uygulanan grupve tiyosemikarbazon-çinko kompleksi uygulanan deneygrubu olmak üzere üç gruba ayrıldı. Uygulamanın 7.,15., 30. ve 45. günlerinde her gruptan 5’er rat ayrılıptartılarak dekapite edildi; kanları alınıp karaciğerağırlıkları kaydedildi. Gerekli analizler otoanalizördespektrofotometrik yöntemle yapıldı.Tiyosemikarbazon ve tiyosemikarbazon-çinko kompleksiuygulanan ratların serum AST, ALT, LDH ve GGTaktiviteleri ile üre, kreatinin, total ve direkt billirubindüzeyleri, ağırlıklarının kontrol grubuna göre istatistiksel olarakönemli bir fark göstermediği (p>0.05) bulundu. Ratlaraderi altı yolla verilen 25 mg/kg tiyosemikarbazon vetiyosemikarbazon-çinko kompleksinin çalışılan serumparametrelerini, karaciğer ağırlığını ve canlı ağırlığıetkilemediği gözlendi.Buna tiyosemikarbazon-çinko kompleksinin tedavi amaçlıkullanıldığında etkilerinden korunulabileceği düşünüldü

Anahtar Kelimeler

• Kan biyokimyasal parametreleri,rat, tiyosemikarbazon ve türevleri

The Effect of Thiosemicarbazone Derivatives on Some Blood Parameters in Rats

Öz

In this study, 25 mg/kg thiosemicarbazoneand 25 mg/kg thiosemicarbazone-zinc complex weregiven to the experiment group rats subcutoneusly at aninterval of three days. 60 rats were used to determine ofthe effect of thiosemicarbazone and thiosemicarbazonezinc complex on the rats, the activities of aspartateaminotransferase (AST), alanine aminotransferase(ALT), lactate dehydrogenase (LDH), gamma-glutamyltransferase (GGT) and levels of urea, creatinine, directbillirubin, total billirubin in serum and liver weight andlive weight of rats.Rats were divided into three groups namely the controlgroup and the two experimental groups exposed tothiosemicarbazone complex.Five rats from the each group at 7th, 15th, 30th, 45thdaysof application were separated and weighed and thendecapitated. Their bloods were taken and their liverweights were recorded. Necessary analyses wereconducted through the autoanalyser and no statisticallysignificant differences were found in activities of AST,ALT, LDH, GGT, and levels of urea, creatinine, totaland direct billirubin in serum of rats exposed tothiosemicarbazone complex, and their liver weight and alive weightsrelative to control group (p>0.05).It has been observed that 25 mg/kg thiosemicarbazoneand 25 mg/kg thiosemicarbazone-zinc complex givensubcutoneusly to the rats did not affect some of theirblood parameters, and liver and live weights of rats.As a result, it was thought that when thiosemicarbazoneand thiosemicarbazone-zinc complex were used for thepurpose of treatment, from their side effects might beavoided by adjusting suitable doses of those substances

Anahtar Kelimeler

• Blood biochemical parameters, rat,thiosemicarbazone derivatives

Kaynakça

1. Byrnes RW, Mohan M, Antholine WE, et al. Oxidative stress induced by a copper- thiosemicarbazone; 29(30): 7046-7053. Biochemistry
2. Perez JM, Matesanz AI, Martin-Ambite A, et al. Synthesis and characterization of com- plexes of p-isopropylbenzaldehyde and methyl -2-pyridyl ketone thiosemicarbazones with Zn (II) and Cd(II) metallic centers, cytotoxic ac- tivity and induction of apoptosis in pam–ros cels. J Inorg Biochem 1999; 75: 255-261.
3. Karatepe M, Karatas F. Antioxidant, pro- oxidant effect of the thiosemicarbazone de- rivative phenylmethylcyclobutane-3-yl)-2-(2- hydroxybenzylidene- hydrazino) thiazole) and its metal complexes on rats. Cell Biochem Funct 2006; 24(6): 547-554. (4-(1
4. Papageorgiou Z, Iakovidou DM, Mioglou E, et al. Antineoplastic and cytogenetic effects of complexes of Pd(II) with 4N-substituted derivatives thiosemicarbazone. Anticancer Res 1997; 17: 247-252. 2-acetyl-pyridine
5. Liu MC, Lin TS, Sartorelli AC. Synthesis and antitumor activity of amino derivatives of pyridine-2-carboxaldehyde zone. J Med Chem 1992; 35(20): 3672-3677. thiosemicarba
6. Chapman JM, Jr Delucy P, Wong OT, et al. Structure activity relationships of imido N- alkyl semicarbazonesi, thiosemicarbazones and acethydrazones as hypolipidemic agents in rodents. Lipids 1990; 25(7): 391-397.
7. Çukurovalı A, Yılmaz I. Synthesis and charac- terization of a new cyclobutane substituted schiff base ligand and its Cd (II), Co (II), Ni (II), and Zn (II) complexes. Pol J Chem 2000; 74: 147-151.
8. Mihich E, Simpson CL, Mulhern AI. Kethoxal bis (thiosemicarbazone) II. toxic and pathologic effects. Cancer Res. 1965, 25: 1417-1431.

9. James RC, Harbison RD, Roberts SM. Phenylpropanolamine potentiation of aceta- minophen-induced hepatotoxicity: evidence for a glutathione-dependent mechanism. Toxicol Appl Pharmacol 1993; 118: 159-168.
10. Kara H, Servi K. Farelerde kadmiyumun akut ve kronik toksitesi üzerine bazı steroid yapılı maddelerin etkisinin araştırılması. F Ü Sağ Bil Derg 1999; 13(3): 249-258.
11. Novelli EL, Silva AM, Monteiro JP, et al. Free radical production by azomethine H: effects on pancreatic and hepatic tissues. Free Radic Res 1997; 26:319-324.
12. Szymanska JA, Swietlica, EA, Piotrowski JK. Protective effect of zinc in the hepatotoxicity of Toxicol 1991; 66(1): 81-91. acetominophen.
13. Tandon SK, Surendra S, Prasad S, et al. Influence of L-lysine and zinc administration during exposure to lead or lead and ethanol in rats. Biol Trace Elem Res 1997; 57(1): 51- 58.
14. Bag S, Vora T, Ghatak R, et al. A study of toxic effects of heavy metal contaminants from sludge-supplemented diets on male wistar rats. Ecotoxicol Environ Saf 1999; 42 (2): 163–170.
15. Hayes PC, Bouchier IAD. Effect of acute and chronic propranolol administration on anti- pyrine and parecetamol clearence in patients with chronic liver disease. Am J Gastroent 1989; 84(7): 723-726.