KÖK KANALLARINDA SIKLIKLA GÖRÜLEN ÜÇ MİKROORGANİZMANIN ELEKTRONİK BURUN SİSTEMİ KULLANILARAK SINIFLANDIRILMASI

Öz

Pulpa ve periradiküler doku hastalıklarının çoğu hem aerop hem de anaerop mikroorganizmaları içeren karışık bir mikroflora ile ilişkilidir. Hekim tedavi ile ilgili etkili bir yöntem belirleyebilmek için mikroorganizma varlığı ile endodontik hastalık arasındaki yakın ilişkiyi kavramalıdır. Endodontik enfeksiyonlardaki bakteri türlerini ortaya çıkarmak kullanılmaktadır. Son yıllarda moleküler yöntemlerdeki gelişmelere rağmen her iki metodun da bir takım dezavantajları bulunmaktadır. Elektronik burun (EB) terimi, insan koku duyusunu taklit edebilen bir elektronik sistemi tanımlar. Elektronik burun sistemleri tıp alanında ve kullanılmaktadır. Bu sistemlerinin en önemli özelliği, koku çeşitlerini çok kısa bir süre içerisinde insan burnundaki hassasiyet derecesinde algılayıp ayrıştırabilmesi ve sonucu objektif olarak sunmasıdır. Bu çalışmada, kök kanal mikroorganizma türünün saf kültüründen elektronik burun sistemi kullanılarak koku verileri alınmıştır. Bu veriler Ayırma sınıflandırılmış, Mahalanobis ve ikinci dereceden fonksiyonlar (quadratic) olmak üzere 3 ayrı metodun performansı incelenmiştir. Mahalanobis ve ikinci dereceden fonksiyonlar kullanarak gerçekleştirilen AA ile sınıflandırmada % 100 başarı elde edilmiştir. Bu çalışma ile EB cihazının, mikroorganizma süspansiyonlarının araştırılmıştır

Anahtar Kelimeler

• Elektronik burun, kök kanal enfeksiyonu, mikrobiyoloji, bakteri, mantar

Classification of Three Microorganism Species Frequently Seen in Root Canals Using Electronic Nose System

Öz

Most diseases of pulp and periradicular tissues are associated with mixed microflora including both aerobic and anaerobic microorganisms. Clinicians must understand the close relationship between the presence of microorganisms and endodontic disease process to determine an effective procedure with respect to the treatment. Culture and molecular methods are used to detect bacterial species in root canal infections. Despite the advances in molecular methods recently, both the culture and the molecular methods have several disadvantages. The term electronic nose describes an electronic system that is able to mimic the human sense of smell. Electronic nose systems have already been used with success in the medical science and microbiological research. The most important property of electronic nose systems is the ability to detect different odour types in a short period of time with almost sensivity of human nose. Furthermore, the result of process using electronic nose system is objective. In this study, odour data was taken by using an electronic nose equipment from pure cultures of three microorganisms which are frequently seen in root canal infections.These datas were classified using Discriminant Analysis (DA) and investigated the performance of several subtypes of DA algorithm namely linear, Mahalanobis and quadratic. The success rate acquired from the classification using Mahalonobis and quadratic approach of DA was 100%. In this study, the use of electronic nose in the classifacation of microorganism suspensions was investigated

Anahtar Kelimeler

• Electronic nose, root canal infection, microbiology, bacteria, fungi

Kaynakça

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