THE EFFECTS OF POMEGRANATE SEED OIL ON CHRONIC LIVER DAMAGE INDUCED CARBON TETRACHLORIDE IN RATS AND DETERMINATION OF HEPATIC APOPTOSIS AND CASPASE ACTIVITY

Öz

In this study, the protective effect, and biochemical values of pomegranate seed oil (PSO) on liver tissue lesions in rats with a chronic liver injury caused by carbon tetrachloride(CCl4)were investigated.Thirty-two rats were divided into 4 groups as 8 rats in each group. The first group was determined as control. The second group received PSO gavage at 2 ml/kg daily for 12 weeks. In the third group, 0.2 ml/kg two doses of CCl4 were injected intraperitoneally at 3-day intervals in each week. The fourth group received PSO at 2 ml/kg daily by gavage for 12 weeks, while 2 doses CCl4 at 3 days intervals in each week. While normal liver tissue structure was observed in the control groups; Macro and microvesicular fat vacuoles were less common in CCl4+PSO groups hepatocytes than CCl4 group. In PSO applications, histopathological changes were partially decreased compared to the CCl4 group, while caspase 3 and 9 activity decreased to a limited number. Serum ALT activity and liver MDA levels were significantly increased in the chronic CCl4 treated groups, while PSO applications were not significantly affected. In the experimental groups treated with chronic CCl4 with PSO, no significant effect was observed on biochemical data and histopathological lesions on liver damage.

Anahtar Kelimeler

• Histopathology
• Immunohistochemistry
• Carbon tetrachloride
• Pomegranate seed oil
• Rat

RATLARDA KARBON TETRAKLORÜR İLE OLUŞTURULAN KRONİK KARACİĞER HASARI ÜZERİNE NAR ÇEKİRDEĞİ YAĞININ ETKİSİ VE KASPAZ AKTİVİTESİ İLE HEPATİK APOPTOZİSİN BELİRLENMESİ

Öz

Bu çalışmada, karbon tetraklorür (CCl4) ile kronik karaciğer hasarı oluşturulan ratlarda nar çekirdeği yağının (NÇY) karaciğer dokusunda oluşabilecek lezyonlar üzerine koruyucu etkisi ve biyokimyasal değerleri araştırılmıştır.Çalışmada 32 adet, her grupta 8 rat olmak üzere 4 gruba ayrıldı. İlk grup kontrol olarak belirlendi. İkinci gruba 12 hafta her gün 2 ml/kg dozunda NÇY gavaj ile verildi. Üçüncü gruba dilüe edilen 0.2 ml/kg iki doz CCl4 her hafta 3 gün ara ile intraperitoneal olarak enjekte edildi. Dördüncü gruba 2 ml/kg dozunda NÇY 12 hafta her gün, gavaj ile verilirken her hafta 3 gün ara ile 2 doz CCl4 uygulandı.Kontrol gruplarında normal karaciğer doku yapısı gözlenirken; CCl4+NÇY grubu hepatositlerde CCl4 grubuna göre daha az yoğunlukta makro ve mikroveziküler yağ vakuolleri görüldü. NÇY uygulamalarında histopatolojik değişiklikler kısmen CCl4 grubuna göre kısmen azalırken, kaspaz 3 ve 9 aktivitesi de sınırlı sayıda azaldı. Kronik olarak CCl4 uygulanan gruplarda serum ALT aktivitesi ve karaciğer MDA düzeyleri önemli düzeyde artış gösterirken, NÇY uygulamaları istatistiki önemde etkilenmedi.Kronik CCl4 ile NÇY verilen deney gruplarında karaciğer hasarı üzerinde biyokimyasal veriler ve histopatolojik lezyonlar üzerine iyileştirici yönden anlamlı bir etkisi gözlenmedi.

Anahtar Kelimeler

• Histopatoloji
• İmmunhistokimya
• Karbon tetraklorür
• Nar çekirdeği yağı
• Rat

Kaynakça

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