LİTYUM TEDAVİSİ GÖREN VE GÖRMEYEN BİPOLAR AFFEKTİF BOZUKLUK TANISI KONULMUŞ HASTALARIN ERİTROSİTLERİNDE SÜPEROKSİT DİSMUTAZ VE KATALAZ DÜZEYLERİNİN İNCELENMESİ

Yıl 2004, Cilt: 13 Sayı: 1, 16 - 20, 01.03.2004

K.muzaffer Üstdal Hülya Haspaylan Recep Saraymen Eser Kılıç

Öz

Bu çalışmada bipolar affektif bozukluklu hastaların eritrosit süperoksit dismutaz (SOD), katalaz (CAT) seviyeleri incelendi. DSM-IV tanı ölçütlerine göre bipolar affektif bozukluk tanısı konmuş 40 hasta ve 20 sağlıklı kişi çalışma kapsamına alındı. Daha önce lityum kullanmamış 20 hasta I. Grubu 2-96 aydır düzenli lityum kullanan 20 hasta II.grubu oluşturdu. I.grup ve II.grup hastalar ile kontrol grubunu oluşturan 20 sağlıklı kişiden alınan kan örneklerinde eritrosit SOD ve CAT seviyeleri ölçüldü. Lityum kullanan gruptaki hastaların lityum dışında ilaç kullanmalarına etik açıdan uygun olmayacağı için müdahale edilmedi. İstatistiki karşılaştırmalarda eritrosit SOD düzeyleri (Ü/gHb) değerlendirildiğinde II.grup değerleri hem I.grup hem de kontrol grubu değerlerinden daha yüksek bulundu (p<0.001). Kontrol grubu değeri ise I.grup değerinden yüksek bulundu (p<0.001). CAT (Ü/gHb) açısından değerlendirildiğinde I.grup değeri II.gruptan yüksek bulunmakla beraber, bu farklılık istatistiki açıdan önemsizdi (p>0.005). I.ve II.grup değerleri kontrol grubu değerlerinden yüksek bulundu (p<0.001). Bu durumda bipolar affektif bozukluklu hastalarda antioksidan sistemin bozulduğu, buna bağlı olarak da eritrosit SOD seviyesinin düştüğü, ancak lityum tedavisi sonunda yükseldiği gözlenmiştir. Sonuç olarak; bipolar affektif bozuklukta eritrosit CAT seviyesinin yükseldiği, lityum tedavisinin ise CAT seviyelerini etkilemediği görülmüştür

Anahtar Kelimeler

Bipolar affektif bozukluk, süperoksit dismutaz, katalaz, lityum tedavisi

Investigation of Erythrocyte SOD and CAT Levels of Patients with Bipolar Affective Disorder Treated with or Without Lithium

Öz

This study was undertaken to examine the role of lithium on antioxidant defence system in patients with bipolar affective disorder. This study was carried on with 40 patients (selected according to OSM scale) and 20 controls. Patients group was subdivided into 2 groups. First group was contained patients who never had lithium in their past. Second group was chosen patients who were regularly taken lithium treatment for the last 2-96 months. During the study patients who had taken lithium treatment, were not restricted fron taking any other medicine during the study, for the ethics of study. The level of erythrocyte SOD was found to be significantly elevated in group II as compared with those from both group I and control (p<0,001). Control group erythrocyte SOD level was also found significantly higher than in goup I (p<0,01). The CAT (Ü/gHb) generatıon by erythrocytes was found increased ?????? in group I as compared with those group II, though this difference was not statistically significant (p>0,005). However CAT generation was higher and statistically different in group I and group II than in control (p<0,001). These findings show that in patients with bipolar affective disorder, antioxidant enzyme system was dejenersted. Thus erythrocyte SOD level was decreased but after lithium treatment it increased. Although CAT activity was increased in bipolar affective patients no effect of lithium on CAT activity was observed

Anahtar Kelimeler

Bipolar affective disorder, superoxide dismutase, catalase, lithium treatment

Kaynakça

• Halliwell B. Oxidants and the central nervous system : Some fundamental questions. Acto Neurol. Scand 1989,126:23-33.
• Lohr JB, MD. Oxygen Radicals and Neuropsychiatric Illness. News and Views. Arch Gen Psychiatry 1991,48:1097-1106. 3. Halliwell B and Gutteridge J. Free Radicals in Biology and Medicine (2nd ed). 1996, pp 11- 21.
• Kılınç K. Oksijen Radikalleri : Üretilmeleri, Fonksiyonları ve Toksik Etkileri. Biyokimya Dergisi 1985,2:59-89. 5. Siest G. Blood activity of Cu-Zn-superoxide dismutase, glutathione peroxidase and catalase in Alzheimers disease : a case- control study. Gerontology . 1990, 36:306- 313.
• Zhow Z, and Kang Y. Cellular and Subcellular Localization of Catalase in the Heart of Transsgenic Mice 2000,48(5):585- 594. 7. Meltzer LH. Is there a specific membrane defect in bipolar disorders ? Biol Psychiatry, 1991,30:1071-1074.
• Uğur M, Özcanlı T, Alıcı Y. Bir şizoaktif vaka dolayısıyla Lithium tedavisinin gözden geçirilmesi. Yeni symposium 2000,38(3):92- 98. 9. Abiaka C, Al-awadi F, Olusi S. Effect of prolonged storage on the activites of superoxide dismutase, Glutathione Reductose and Glutathione Peroxidase. Clinical Chemistry 2000,46(4):566-567.
• Webster N, Tothill C. Effects of Blood Storage on Red Cell Antioksidative Ssystems. Acta Haemat 1986,75:30-33.
• Sun Y, Oberley LW, Yong Y. A Simple Method for Clinical Assoy of Superoxide Dismutase. Clin Chem 1988,34(3):497-500.
• Durak Yurtaslanlı Z, Conbalat O ve ark. A J, methodological approach to superoxide dismutase (SOD) activity assay based on inhibition of nitroblue tetrazolium (NBT) reduction. Clinica Chimica Acta 1993;21 Sun Y, Oberley LW. Suitability of copper chloride as a reaction terminaton for superoxide dismutase activity assay. Clinica Chimica Acta 1994,226:101-103.
• Sun Y, Oberley LW. Suitability of copper chloride as a reaction terminaton for superoxide dismutase activity assay. Clinica Chimica Acta 1994,226:101-103.
• Kıkuchi-Torrii K, Hayashi S, Nakamoto H, et al. Properties of Aspergillus Niger Catalase. J Biochem 1982,92:1449-1456.
• Fairbanks VF, Klee GG. Biochemical aspects of hematology. In : Burtis CA, Ashwoad ER (eds). Tietz Textbook of Clinical Chemistry (3rd ed). WB Saunders Philadelphia 1999, pp 1673-1674.
• Delibaş N, Özcankaya R, Özgüner F ve ark. Bilişsel durum değişimleri, depresif ve psikotik belirtilerle serbest radikal aktvitesinin ilişkisi. Türk Psikiyatri Dergisi 1996, 7(1) : 46-52.
• Güzelhan Y, Sayar K, Öztürk M ve ark. Şizofrenide serbest radikaller. Klinik Psikofarmakoloji Bülteni, 2000,10(2):90-96.
• Cengiz M, Yüksel A, Seven M. The effects of carbamazepine and valproic asid on the erythrocyte glutatione, glutathione peroxidase, superoxide dismutase and serum lipid peroxidation in epileptic children. Pharmacological Research 2000,41(4):423- 425.
• Buckman TD, Kling AS, Eiduson S. et al. Glutathione peroxidase and CT scan abnormalities in schizophrenia. Biol Psychiatry 1987,22:1349-1356.
• Konuk N, Kocabaşoğlu N. Bipolar affektif bozuklukta güncel tedaviler. Yeni symposium 2000,38(2):56-62.