YABAN VE EVCİL ÖRDEKLERDE PREEN BEZİ ÜZERİNDE HİSTOMORFOLOJİK VE HİSTOKİMYASAL ÇALIŞMALARI

Yıl 2005, Cilt: 14 Sayı: 1, 20 - 30, 01.03.2005

Melek Koçak Harem Hikmet Altunay İsmail Şah Harem Feyzullah Beyaz

Öz

Bu araştırma, yaban ve evcil ördeklerin preen bezlerinin histomorfolojik ve histokimyasal yönden incelenerek aralarındaki farklılıkları ortaya koymak amacıyla yapıldı. Çalışmada beşer adet erişkin erkek yaban ve evcil Pekin ördeğinin preen bezi materyal olarak kullanıldı. Her iki türde bezin merkezi bir akıtıcı kanala açılan tubullerden oluştuğu, yaban ördeğinde parenşim/stroma oranının parenşim lehine evcil ördektekinden daha büyük olduğu belirlendi. Yaban ve evcil ördekte glikojen bölgesindeki tubuller arası bağdoku alanlarında lenfosit infiltrasyonlarına rastlandı. Yaban ördeğinde ayrıca kapsül ve glikojen bölgesindeki tubuller arasındaki bağdokuda Herbst cisimciklerinin bulunduğu gözlendi. ANAE aktivitesinin iki türün glikojen bölgesinde aynı, yağ bölgesinde farklı olduğu; asit fosfatazın ise sadece yaban ördeğinin glikojen bölgesinde yoğun olarak bulunduğu saptandı. Her iki türde bezin yağ bölgesindeki tubul epitelinin tüm katmanlarının nötral yağları içerdiği, sudanofilik yağların yaban ördeğinde yağ bölgesindeki tubul epitelinin tüm katmanlarında, evcil ördekte ise süperfisiyel hücrelerle hemen bunların altında bulunan intermediyer hücrelerde bulunduğu tespit edildi

Anahtar Kelimeler

Evcil ördek, yaban ördeği, preen bezi, histoloji, histokimya

Histomorphological and Histochemical Studies on Preen Gland of the wild and Domestic Duck

Öz

The aim of this study is to investigate the differences of preen gland by histomorphologically and histochemically in the wild and domestic duck. Preen glands of five mature, male wild ducks and Pekin ducks were used as materials in the investigation. It was determined in the both species that gland was composed of tubules that opened a central duct and proportion of parenchyma/stroma was in favour of parenchyma in wild duck which was more high than domestic duck. In the wild and domestic ducks were found of lymphocytic infiltration in the intertubuler intersititium of glikogen zone. Also it was observed in the wild duck that capsule and intertubuler intersititium of glycogen zone were included Herbst corpuscle. It was determined that in the both species, periyodic acid-schiff and αnaphtyl acetate esterase reactivities were the same in the glycogen zone but different in the sebaceous zone; whereas, reactivity of acid phosphatase was found to be strong in the glycogen zone in the preen gland of wild duck only. It was determined in the both species that epithelial layers of tubules in the sebaceous zone were included neutral lipids totally. Sudanophilic lipids were found in the epithelial layers of tubules totally in the sebaceous zone of the wild duck whereas the superficial cells and intermediate cells, nearly located beneath of the superficial cells in the domestic duck

Anahtar Kelimeler

Domestic duck, wild duck, preen gland, histology, histochemistry

Kaynakça

• Dellmann HD, Brown EM. Textbook of Veterinary Histology (3th Ed). Williams and Wilkins, Philadelphia 1987, pp 46-47.
• Kolattukudy PE. Avian uropygial (preen) gland. Method Enzymol 1981, 72: 714-720
• Zık B, Erdost H. Horozlarda acı kırmızı biber- li rasyonla beslemenin üropigi bezi üzerine etkisinin histolojik yönden incelenmesi. Turk J Vet Anim Sci 2002, 26: 1223-1232.
• Bell DJ, Freeman BM. Phsiyology and Biochemistry of the Domestic Fowl. Vol. 1 Academic Pres, London, New York 1971, pp 93-164.
• Hodges RD. The Histology of the Fowl. Academic Pres Inc., London 1974, pp 113-146.
• Yu QT, Liu BN, Zhang JY, Huang ZH. Location of methyl branchings in fatty acids: Fatty acids in uropygial secretion of Shanghai duck by GC- MS of 4,4-dimethyloxazoline derivatives. Lipids 1988, 23 (8): 804-810.
• Damste JSS, Dekker M, Van Dongen BE, Schouten S, Piersma T. Structural identification of the diester preen-gland waxes of the Red Knot (Calidris canutus). J Nat Prod 2000, 63: 381-384.
• Sandilans V, Powell K, Keeling L, Savory CJ. Preen gland function in layer fowls; factors affecting preen oil fatty acid composition. Brit Poultry Sci 2004, 45 (1): 109-115.
• Bhattacharyya SP, Ghosh A. Histochemical studies on the enzymes of the uropygial gland. Acta Histochem Bd 1971, 39: 318-326.
• Chem.Shawkey MD, Pillai SR, Hill GE. Chemical warfare? Effects of uropygial oil on feather-degrading bacteria. J Avian Biol 2003, 34: 345-349.
• Bhattacharyya SP, Sahu C. Histomorphological and histochemical studies on the preen gland of cortisone-treated male pigeons. Anat Anz Bd 1976, 140: 162-169.
• Maiti BR, Ghosh A. Cytomorphological and cytochemical studies of the uropygial gland of the scorbutic bulbul, Pycnonotus Cafer. Acta Histochem Bd 1972, 42: 217-229.
• Abalain JH, Amet Y, Daniel JY, Floch HH. Androgen control of the secretion in the sebaceous-like preen gland. J Steroid Biochem 1984, 20 (1): 529-531.
• Abalain JH, Amet Y, Daniel JY, Floch HH. Androgen regulation of secretions in the sebaceous-like uropygial gland of the male Japanese quail. J Endocr 1984, 103: 147-153.
• Abalain JH, Amet Y, Lecaque D, Secchi J, Daniel JY, Floch HH. Ultrastructural changes in the uropygial gland of the male Japanese quail, Coturnix coturnix, after testosterone treatment (Comparision with the sebaceous gland of the male rat). Cell Tissue Res 1986, 246: 373-378.
• Sjöberg K, Pöysa H, Elmberg J, Nummi P. Response of Mallard duckling to variation in habitat quality: An experiment of food limitation. Ecology 2000, 81(2): 329-335.
• Watkins EJ, Butler PJ, Kenyon BP. Posthatch growth of the digestive system in wild and domesticated ducks. Br Poult Sci 2004 Jun, 45 (3): 331-341.
• Kalisinska E. Comparison of hemispher size in wild and domestic geese. Anat Histol Embryol 1997, 26: 3-9.
• Kolattukudy PE, Bohnet S, Rogers L. Disappearance of short chain acids from the preen gland wax of male Mallard ducks during eclipse. JLR 1985, 26: 989-994.
• Crossmon O. A modification of Mallory’s connective tissue stain with a discussion of the principles involued. Anat Rec 1937, 69: 31- 38.
• Bancroft JD, Stevens A, Turner DR. Theory and Practice of Histological Techniques (3th ed). Churchill Livingstone, 1990, pp 196-221- 223 -386-390.
• Sandpiper. Reneerkens J, Piersma T, Damste JSS. Sandpipers (Scolopacidae) switch from monoester to diester preen waxes during courtship and incubation, but why? Proc R Soc Lond B 2002, 269: 2135-2139.
• Gözükara E. Biyokimya 2 2. baskı Malatya, 1994.
• Downie MT, Kealey T. Lipogenesis in the human sebaceous gland: Glycogen and glycerophosphate are substrates for the synthesis of sebum lipids. J Invest Dermatol 1998, 111: 199-205.
• Guyton AC, Hall JE. Textbook of Medical Physiology (10th ed). W. B. Saunders Company, 2000, pp 13
• Conroy JD, Gren CA. Distrubution of acid and alkaline phosphatases in canine skin. Am J Vet Res 1975 Dec 36 (12): 1697-1703.