KAYSERİ’DE TÜKETİME SUNULAN KANATLI ETLERİNDEN ARCOBACTER SPP’NİN İZOLASYONU

Yıl 2005, Cilt: 14 Sayı: 2, 125 - 132, 01.06.2005

Fatma Ok Anadut K.semih Gümüşsoy

Öz

Bu araştırmada, Kayseri ilindeki farklı satış noktalarından alınan tavuk, hindi ve bıldırcın karkaslarında Arcobacter spp.’nin varlığı araştırıldı. Tavuk karkas numunelerini bütün tavuk, but, göğüs, kanat ve baget; hindi numunelerini göğüs ve kanat oluştururken; bıldırcın numuneleri ise bütün olarak çalışmada kullanıldı. Bu amaçla, Kayseri ilindeki 5 marketten toplam 50 adet tavuk, 50 adet hindi ve 50 adet bıldırcın karkas örneği toplandı. Numunelerin her biri steril distile suda yıkandıktan sonra Arcobacter Enrichment Broth (AEB) içerisinde mikroaerobik koşullarda 30 oC’de kültüre edildi. Daha sonra kanlı agar (KA) üzerine yerleştirilmiş 0.45 µm selüloz asetat membran filtreler kullanılarak membran filtrasyon tekniği uygulandı. Araştırmada, bütün tavuk numunesinde 5/9 (% 25), but numunesinde 4/10 (% 20), göğüs numunesinde 5/9 (% 25), kanat numunesinde 2/10 (% 10), baget numunesinde 4/12 (% 20); hindilerden ise kanat numunelerinden 1/25 (% 4) ve göğüs numunelerinden 1/25 (% 4) ve bütün olarak incelenen bıldırcın numunelerinden 4/50 (% 8) Arcobacter spp. izole edildi. Sonuç olarak; Kayseri ilinde bulunan marketlerde satışa sunulan taze kanatlı karkaslarının genellikle Arcobacter spp.’lerle kontamine olduğu saptandı. İnsanlarda başta enterit olmak üzere diğer hastalık olgularından da izole edildikleri göz önünde bulundurulduğunda etkenin halk sağlığı açısından önemli bir risk faktörü oluşturabileceği sonucuna varıldı

Anahtar Kelimeler

Arcobacter spp., tavuk, hindi, bıldırcın, izolasyon

Isolation of Arcobacter spp. From Poultry Carcasses Put on Consumption in Kayseri

Öz

In this study, the presence of Arcobacter spp. was examinated in carcasses of chicken, turkey and quail sold different markets in Kayseri. While carcasses samples of chickens included whole chicken rump, breast, wing and little rump; samples of turkeys included breast and wing; samples of quail was used as a whole in this study. For this aim; 50 chickens, 50 turkeys and 50 quails carcasses samples were collected from 5 markets in Kayseri. After each sample was washed in sterile distilled water and inoculated at 30°C under microaerobic conditions in Arcobacter Enrichment Broth (AEB). Membrane filtration technique was applied to washing liquid by using cellulose acetate membrane filter which has 0.45 µm pore diamater and was put on blood agar (BA). In this research, 5/9 (% 25) chickens samples, 4/10 (% 20) rumps samples, 5/9 (% 25) breast samples, 2/10 (% 10) wings samples, 4/12 (% 20) drumstick samples and from turkeys 1/25 (% 4) wings samples and 1/25 (% 4) breast samples and 4/50 (% 8) quail samples which is investigated as a whole Arcobacter spp. was isolated. The study revealed that fresh poultry carcasses was contaminated with Arcobacter spp. Sold in Kayseri when considered that Arcobacter spp. Was isolated from many disease in addition to human enteritis. It was concluded that Arcobacter spp. Might be an important risk factor as far as public health is concerned

Anahtar Kelimeler

Arcobacter spp., chicken, Turkey, quail, isolation

Kaynakça

• 1. Çelik S. Arcobacter. Seminer, Ankara Ün Sağlık Bilimleri Enstitüsü, Ankara, 2000.
• 2. Houf K, Devriese Luc A, Zutter LD, Hoof JV, Vandamme P. Development of a new protocol for the isolation and quantification of Arcobacter species from poultry products. Int J Food Microbiol 2001; 71: 189-196.
• 3. Antolin A, Gonzales I, Garcia T, Hernandez P. E, Martin R. Arcobacter spp. enumeration in poultry meat using a combined PCR-ELISA assay. Meat Sci 2001; 59: 169-171.
• 4. Vandamme P, Falsen E, Rossau R, et al. Revision of Campylobacter, Helicobacter and Wolinella taxonomy: Emendation of generic descriptions and proposal of Arcobacter gen. Int J Syst Bacteriol 1991; 41: 88-103.
• 1. Aydın F, Atabay Hİ. Arcobacter türleri; sınıflandırma, genel özellikler, izolasyon ve identifikasyon metotları. Vet Hek Mikrobiyol Derg 2001; 1: 71-76.
• 2. Long C, Phillips C. A. The effect of sodium citrate, sodium lactate and nisin on the survival of Arcobacter butzleri NCTC 12481 on chicken. Food Microbiol 2003; 20: 495- 502.
• 3. Phillips CA, Arcobacter spp in food: isolation, identification and control. Trends Food Sci Tech 2001; 12: 263-275
• 4. Atabay Hİ. Kars ili ve yöresinde bulunan marketlerde satışa sunulan tavuk karkaslarında Arcobacter türlerinin prevalansının saptanması ve elde edilen suşların PCR-RAPD tekniği kullanılarak tiplendirilmesi üzerinde araştırma. TOGTAG/TARP-2376 nolu proje. Kars, 2001.
• 5. Vanderzant C, Splittstoesser DF. Compendium of methods for the microbiological examination of foods (3 th ed), American Public Health Association, NW Washington, DC 1992, pp 112-360.
• 6. Bilgehan H. Klinik Mikrobiyolojik Tanı (2. Baskı), Fakülteler Kitabevi Barış Yayınları, İzmir 1995, ss 67-680.
• 7. Özdamar K. SPSS ile Biyoistatistik (4. Baskı), Kaan Kitabevi, Eskişehir 2001, ss 343-360.
• 8. de Oliveira SJ, Moraes HL, Kuchenbecker BS, et al. Isolation of Arcobacter spp. from poultry carcasses, in Brazil. Ciencia Rural 2001; 31: 639-643.
• 9. Rivas L, Fegan N, Vanderlinde P. Isolation and characterisation of Arcobacter butzleri from meat. Intern J Food Microbiol 2004; 91:
• 10. de Boer E, Tilburg JJ, Woodward DL, Lior H, Johnson W. A selective medium for the isolation of Arcobacter from meats. Let Appl Microbiol 1996; 23: 65-66.
• 11. Marinescu M, Collignon A, Squinazi F, et al. Two cases persistent diarrhoea associated with Arcobacter spp., Campylobacters, Helicobacters and related organisms. ed: Newell DG, Ketley J, Feldman RA. Plenum Publishing Corporation, New York, 1996, pp 521-523.
• 12. Houf K, Devriese Luc A, Zutter LD, Hoof JV, Vandamme P. Susceptibility of Arcobacter butzleri, Arcobacter cryaerophilus, Arcobacter skirrowii to antimicrobial agents used in selective media. J Clin Microbiol 2001; 39: 1654-1656.
• 13. Golla SC, Murano EA, Johnson LG, et al. Determination of the occurrence of Arcobacter butzleri in beef and dairy cattle from Texas by various isolation methods. J Food Prot 2002; 65: 1849-53.
• 14. Driessche EV, Houf K, Vangroenweghe F, et al. Occurrence and strain diversity of Arcobacter species isolated from healthy Belgian pigs. Res Microbiol 2004; 155: 662- 666.
• 15. de Oliveira SJ, Baetz AL, Wesley IV, Harmon KM. Classification of Arcobacter species isolated from aborted pig fetuses and sows with reproductive problems in Brazil. Vet Microbiol 1997; 57: 347-354.
• 16. Morita Y, Maruyama S, Kabeya H, et al. Isolation and phylogenetic analysis of Arcobacter spp. ground chicken meat and environmental water in Japan and Thailand. Microbiol Immunol 2004; 48: 527-533.
• 17. Ridsdale JA, Atabay Hİ, Corry JL. Campylobacter and Arcobacter spp. isolated from the carcasses and caeca of commercially reared ducks. Anaerobe 1999; 5: 317-320.
• 18. Atabay Hİ, Aydın F, Houf K, Şahin M, Vandamme P. The prevalence of Arcobacter spp. on chicken carcasses sold in retail markets in Turkey and identification of the isolates using SDS-PAGE. Int J Food Microbiol 2003; 81: 21-28.