TAVŞANLARDA SWİM-UP YÖNTEMİNİN YAVRU CİNSİYET ORANLARI ÜZERİNE ETKİSİ

Öz

Bu çalışmada tavşanlarda swim-up yönteminde farklı inkübasyon sürelerinin yavru cinsiyet oranları üzerine etkisi araştırıldı. Araştırmada hayvan materyali olarak; 108 adet dişi, 8 adet erkek Yeni Zelanda Beyazı tavşan kullanıldı. Sperma erkek tavşanlardan suni vagen yöntemi ile alındı. Spermalara yıkandıktan sonra, 15 (grup 1), 30 (grup 2) ve 45 (grup 3) dakika (dak) süre ile swim-up yöntemi uygulandı. Dişi tavşanlar Swim-up gruplarında inkübasyon (37.5 oC’de, % 5 CO2’li havada) sonunda spermanın üst ½’lik kısmı ile, kontrol gruplarında ise spermanın tamamı ile intrauterin yolla tohumlandı (grup 1K, 2K, 3K). Tohumlama anında dişi tavşanlara intramusküler (IM) yolla 0,0042 mg Buserelin asetat (Receptal® Intervet, Germany) enjekte edildi. Yavru cinsiyetleri doğum sonrasında kaydedildi. Gebelik oranları 1, 2 ve 3. gruplarda sırasıyla % 33,33, % 22,22 ve % 50 (p: 0,214); kontrol gruplarında (grup 1K, 2K, 3K) ise % 50, % 44,44 ve % 44.44 olarak tespit edildi (p: 0,928). Yavru cinsiyetleri % erkek oranı olarak değerlendirildiğinde; 1., 2. ve 3. gruplarda sırasıyla % 65.7, % 30 ve % 51,7 (p: 0,038); kontrol gruplarında ise %50,9, %46,7 ve % 37,50 olarak belirlendi (p: 0,480). Sonuç olarak tavşanlarda swim-up yöntemi uygulanan spermalarda, kısa süreli inkübasyon ve spermanın üst bölümünün kullanılması ile yavru cinsiyet oranlarının değiştirilebileceği kanaatine varıldı

Anahtar Kelimeler

• Tavşan, swim-up, cinsiyet oranı

The Effect of Swim-Up Technique on Sex Ratio of Newborns in Rabbits

Öz

The present study investigates the effect of different incubation periods on gender ratios of offspring in rabbits in the swim-up technique. 108 female and 8 male New Zealand White rabbits were used in the study as an animal subject. Ejaculates were collected from the male rabbits by using an artificial vagen. The washed sperms were applied using the swim-up technique for periods of 15(group1), 30 (group2), and 45 (group3) minutes. Female rabbits were inseminated by the upper ½ part of the semen in the swim-up groups (groups 1, 2 and 3), while the control groups were inseminated using the whole semen (groups 1K, 2K and 3K) at the end of incubation (at 37.5oC, under 5 % CO2 in air) . Doses were injected intramuscularly with 0.0042 mg Buserelin asetat (Receptal® Intervet, Germany) at the time of insemination. The sex ratios of offsprings were recorded after parturitions. The pregnancy rates were detected as 33.33%, 22.22 % and 50.00% (p=0.214) in groups 1, 2, and 3 respectively. As for the control groups, the pregnancy rates were 50.00%, 44.44% and 44.44% (p=0.928) for groups 1K, 2K and 3K, respectively. The male sex ratios of the newborns were 65.70%, 30.00% and 51.70% (p=0.038) in groups 1, 2 and 3 respectively. In the control groups (1K, 2K and 3) the male sex ratios were 50.90%, 46.70% and 37.50% (p=0.480), respectively. It was concluded that the sex ratio of offsprings in rabbits could be manipulated in the swim-up technique by using the upper part of the semen and applying a shorter incubation period

Anahtar Kelimeler

• Rabbit, swim-up, sex ratio

Kaynakça

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