Clitocybe geotropa TÜRÜ MANTARDAN ELDE EDİLEN EKSTRAKTLARIN MDA-MB-231 HÜCRE HATTI ÜZERİNDEKİ SİTOTOKSİK AKTİVİTESİNİN İNCELENMESİ

Yıl 2017, Cilt: 26 Sayı: 1, 0 - 0, 01.03.2017

Berrak Altınsoy S.furkan Konca Huriye Aksu Büşra Kurt Hakan Allı Çağrı Şakalar

Öz

Genel Bilgi: Mantarların tıbbi amaçlı olarak kullanımlarının geçmişi Asya bölgesi için oldukça eskiye dayanmaktadır ve son zamanlarda Batı’da kullanımları Kabul
görmektedir. Makro mantarlardan izole edilen bileşenlerin veya ekstrelerin antimikrobiyal, anti-viral,
antitümör, anti-alerjik, immünomodülatör, anti- enflamatuar, anti-aterojenik, hipoglisemik ve hepatoprotektif etki gibi çeşitli biyolojik aktivitelerinin olduğu
bildirilmiştir.
Amaç: Bu çalışmada, Clitocybe geotropa (Bull.)
Quél.’den elde edilen etanol ve metanol ekstrelerinin
meme kanseri hücreleri üzerine etkinliği araştırılmıştır.
Yöntem: Bu çalışmada mantar ekstrelerinin verimi,
belli ağırlıktaki kuru materyalden elde edilen etanol
ekstresinin (% 28,2) ve metanol ekstresinin (% 30,6)
ağırlıklarına göre g/100g olarak ifade edilerek belirlenmiştir. Ekstrelerin sitotoksik etkileri Trypan Blue analiziyle belirlenmiştir.
Bulgular: Etanol ve metanol ekstrelerinin total fenolik
içeriği sırasıyla 217,15±0,02 ve 138,39±0,01; total flavanoit içeriği 152,28±0,03 ve 111,95±0,02 mgCE/g
olarak ölçülmüştür. C. geotropa için 50–400 µg/mL
konsantrasyon değerlerindeki metanol ekstrelerinin
MDA-MB-231 hücre hattı üzerinde sitotoksik etkisi
gözlenmezken, etanol ekstresi % 51,5; 400 μg/ml, %
17,9; 200 μg/ml, % 6,6; 100 μg/ml uygulanan konsantrasyonlarda anlamlı düzeyde sitotoksik etki göstermiştir.
Sonuç: Clitocybe geotropa MDA-MB-231 hücre hattı
üzerinde etkili antikanser ajan olarak kullanılma potansiyeline sahip bir mantar türüdür.

Anahtar Kelimeler

C. geotropa, toplam fenolik, toplam flavanoit, sitotoksite

Cytotoxic Activity of Two Extracts from the Mushroom Clitocybe Geotropa in Mda-Mb-231Cell Line

Öz

Background: The medical use of mushrooms has a very
long tradition in Asia and is nowadays becoming more
accepted in the West. It is reported that the extracts or
isolated constituents from macrofungi have biological
activities such as antimicrobial, anti-viral, anti-tumour,
anti-allergic, immunomodulating, anti-inflammatory,
antiatherogenic, hypoglycaemic and hepatoprotective
activities.
Objective: In this study, cytotoxic activity of ethanol
extracts (EE) and methanol (ME) of Clitocybe geotropa
on MDA-MB-231 cell line were investigated.
Method: The yield of mushroom extracts was calculated for EE and ME 30.6% and 28.2%, respectively.
Cytotoxic effects of the extracts were determined by
Trypan Blue assay. Results: The total phenolic contents of EE and ME were
217.15±0.02, 138.39±0.01; total flavonoid contents
152.28±0.03, 111.95±0.02 mgCE/g, respectively. The
ME of mushroom’s 50–400 µg/mL concentrations did
not show any cytotoxic effect in MDA-MB-231 cell line
while the EE of C. geotropa % 51,5; 400 μg/ml, % 17,9;
200 μg/ml, % 6,6; 100 μg/ml had cytotoxicity.
Conclusion: The mushroom, Clitocybe geotropa, could
be used as a potential anticancer agent for MDA-MB- 231 cell line.

Anahtar Kelimeler

C. geotropa, total phenolic, total flavonoid, cytotoxicity

Kaynakça

• 1. Siegel RL, Miller KD, Jemal A. 2015 Cancer statistics. CA Cancer J Clin 2015; 65 (1): 5-29.
• 2. Wasser SP, Weis AL, Medicinal properties of substances occurring in higher basidiomycetes mushrooms: Current perspectives (review). Int J Med Mushroom, 1999; 1(1).
• 3. Lindequist U, Niedermeyer TH, Jülich WD. The pharmacological potential of mushrooms. Evidence-Based Complem Alter Med 2005; 2 (3):285-299.
• 4. Chan GCF, Chan WK, Sze DMY. The effects of β- glucan on human immune and cancer cells. J Hemat Oncol 2009; 2(1):1-11.
• 5. Zhong JJ, Xiao JH. Secondary metabolites from higher fungi: discovery, bioactivity, and bioproduction, Biotechnology in China I., Springer, Berlin Heidelberg 2009; pp 79-150.
• 6. Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecul 2010; 15(10):7313-7352.
• 7. Kim MY, Seguin Y, Ahn P, et al. Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea. J Agric Food Chem 2008; 56(16): 7265-7270.
• 8. Arfors KE, Ley K. Sulfated polysaccharides in inflammation. J Lab Clin Med 1993; 121 (2):201- 202.
• 9. Singleton VL, Orthofer R, Lamuela-Raventos RM, Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin- Ciocalteu reagent. Meth Enzymol 1999; 299:152- 178.
• 10. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999; 64(4): 555-559.
• 11. Tel G, Apaydın M, Duru ME, et al. Antioxidant and cholinesterase inhibition activities of three Tricholoma species with total phenolic and flavonoid contents: The edible mushrooms from Anatolia. Food Anal Meth 2012; 5(3): 495-504.
• 12. Cheung L, Cheung PC, Ooi VE. Antioxidant activity and total phenolics of edible mushroom extracts. Food Chem 2003; 81(2):249-255.
• 13. Choi SW, Sapers GM. Effects of washing on polyphenols and polyphenol oxidase in commercial mushrooms (Agaricus bisporus). J Agric Food Chem 1994; 42(10):2286-2290.
• 14. Zhu XL, Chen AF, Lin ZB. Ganoderma lucidum polysaccharides enhance the function of immunological effector cells in immunosuppressed mice. J Ethnopharma 2007; 111(2):219-226.
• 15. Sliva D, Sedlak M, Slivova V, et al. Biologic activity of spores and dried powder from Ganoderma lucidum for the inhibition of highly invasive human breast and prostate cancer cells. The J Alter Complement Med 2003; 9(4):491-497.
• 16. Jiang J, Slivova V, Harvey K, et al. Ganoderma lucidum suppresses growth of breast cancer cells through the inhibition of Akt/NF-κB signaling. Nutr Cancer 2004; 49(2):209-216.
• 17. Szeto YT, Lau PC, Kalle W, et al. Direct human DNA protection by Coriolus versicolor (Yunzhi) extract. Pharmaceut Bio 2013; 51(7):851-855.
• 18. Ho CY, Kim CF, Leung KN, et al. Differential anti- tumor activity of coriolus versicolor (Yunzhi) extract through p53-and/or Bcl-2-dependent apoptotic pathway in human breast cancer cells. Cancer Bio Therapy 2005; 4(6):638-644.
• 19. Jin CY, Kim GY, Choi YH. Induction of apoptosis by aqueous extract of Cordyceps militaris through activation of caspases and inactivation of Akt in human breast cancer MDA-MB-231 cells. J Micro Biotech 2008; 18(12):1997-2003.
• 20. Hetland G, Johnson E, Lyberg T, et al. The mushroom Agaricus blazei Murill elicits medicinal effects on tumor, infection, allergy, and inflammation through its modulation of innate immunity and amelioration of Th1/Th2 imbalance and inflammation. Adv Pharmacol Sci 2011; 1-11.
• 21. Wang CR, Ng TB, Li L, et al. Isolation of a polysaccharide with antiproliferative, hypoglycemic, antioxidant and HIV‐1 reverse transcriptase inhibitory activities from the fruiting bodies of the abalone mushroom Pleurotus abalonus. J Pharma Pharmacol 2011; 63(6): 825- 832.
• 22. Zhang M, Cheung PC, Zhang L. Evaluation of mushroom dietary fiber (nonstarch polysaccharides) from sclerotia of Pleurotus tuber- regium (Fries) singer as a potential antitumor agent. J Agric Food Chem 2001; 49(10):5059- 5062.
• 23. Zheng S, Liu Q, Zhang G, et al. Purification and characterization of an antibacterial protein from dried fruiting bodies of the wild mushroom Clitocybe sinopica. ACTA Biochim Polonica 2010; 57(1):43-48.
• 24. Nitha B, Meera C, Janardhanan K. Antiinflammatory and antitumour activities of cultured mycelium of morel mushroom, Morchella esculenta. Current Sci (00113891) 2007; 92(2):235 -239.
• 25. Antonyuk V, Klyuchivska OY, Stoika R. Cytotoxic proteins of Amanita virosa Secr. mushroom: purification, characteristics and action towards mammalian cells. Toxicon 2010; 55(7):1297-1305.