SEROTONERJIK VE NORADRENERJIK MODÜLASYONUN ROSMARINIK ASIT ANALJEZISINE KATKISI

Yıl 2017, Cilt: 26 Sayı: 1, 52 - 59, 01.03.2017

Duygu Aydın Nurcan Bektaş Türkmen , Rana Arslan

Öz

Bu çalışmada, 0.3, 1 ve 3 mg/kg (p.o.) rosmarinik asitin
farelerde santral analjezik etkinliğinin sıcak-plaka ve
kuyruk-batırma testleri, periferal analjezik etkinliğinin
ise asetik asit ile indüklenen kıvranma-testi ile değerlendirilmesi planlanmıştır. Ayrıca bu etkinliğe 5HT2A/2C
reseptör antagonisti ketanserin (1 mg/kg, i.p.) kullanılarak serotonerjik ve α2-adrenerjik antagonisti yohimbin (1 mg/kg, i.p.) kullanılarak noradrenerjik
modülasyonun katkısının araştırılması amaçlanmıştır.
Sıcak-plaka testinde 1 mg/kg rosmarinik asit 45. dakikada, 3 mg/kg rosmarinik asit ise 30., 45. ve 60. dakikada, kuyruk-batırma testinde 1 ve 3 mg/kg rosmarinik
asit 45 ve 60. dakikada termal uyarana karşı verilen
cevabının süresini uzatmıştır. Kıvranma-testinde ise,
45. dakikada uygulanan rosmarinik asit tüm dozlarda
kıvranma sayılarını anlamlı olarak azaltmıştır. Ketanserin, rosmarinik asitin oluşturduğu etkiyi sıcak-plaka
ve kıvranma-testinde değiştirmemiş, kuyruk-batırma
testinde ise anlamlı olarak geri çevirmiştir. Yohimbin,
rosmarinik asitin oluşturduğu etkiyi sıcak-plaka
testinde göreceli olarak, kuyruk-batırma ve kıvranma- testinde ise anlamlı olarak geri çevirmiştir. Rosmarinik
asit hem inici inhibitör yolakta yer alan serotonerjik ve
noradrenerjik sistemi değişik düzeylerde modüle
ederek, hem de periferik noradrenerjik sistemi stimüle
ederek santral ve periferal analjezik etki sağlamaktadır.
Sonuç olarak, ağrının eşlik ettiği rahatsızlıklarda yardımcı ilaç olarak kullanılabilecek doğal bir ilaç adayıdır.

Anahtar Kelimeler

Rosmarinik asit, ağrı, serotonin, noradrenalin

The Participation of Serotonergic and Noradrenergic Modulation in Rosmarinic Acid-Induced Analgesia

Öz

It was aimed to evaluate the central analgesic activity of
0.3, 1 and 3 mg/kg (p.o.) rosmarinic acid in mice by hot- plate and tail-immersion tests in a time dependent
manner and the peripheral analgesic activity by acetic
acid-induced writhing-test. Furthermore one of the
other purpose of the study is to research the involvement of serotonergic and noradrenergic modulation on
rosmarinic acid-induced analgesia by using 1 mg/kg
(i.p.) ketanserin, 5HT2A/2C receptor antagonist, and yohimbine, α2-adrenoceptor antagonist, respectively. The
enhanced withdrawal responses to thermal stimuli
were observed 45 minutes after the administration of 1
mg/kg rosmarinic acid; 30, 45 and 60 minutes after the
administration of 3 mg/kg rosmarinic acid in hot-plate
test; 45 and 60 minutes after the administration of 1
and 3 mg/kg rosmarinic acid in tail-immersion test. In
writhing-test, the number of writhing significantly decreased 45 minutes after the administration of 0.3, 1
and 3 mg/kg rosmarinic acid. Rosmarinic acid-induced
antinociception was significantly reversed by ketanserin only in tail-immersion test while it was reversed
significantly in both tail-immersion and writhing-test
by yohimbine. Rosmarinic acid provides analgesic effects by modulating serotonergic and noradrenergic
systems that are located in descending inhibitory pathway and by stimulating peripheral noradrenergic system. Therefore, rosmarinic acid is a natural agent that
can be used safely as a co-adjuvant in pain management

Anahtar Kelimeler

Rosmarinic acid, pain, serotonin, noradrenalin

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