SIĞIRLARDA EMBRİYO TRANSFERİNDE CIDR İLE SENKRONİZE EDİLEN DONÖRLERE ÖSTRUS ÖNCESİ GERÇEKLEŞTİRİLEN ÇİFT PGF2α UYGULAMALARININ ELDE EDİLEN EMBRİYOLARIN KALİTESİ VE SAYISI ÜZERİNE ETKİLERİ

Yıl 2017, Cilt: 26 Sayı: 3, 204 - 210, 01.12.2017

Uğur Kara Tayfur Bekyürek

Öz

Bu çalışmada, sığırlarda embriyo transferinde östrus
öncesi uygulanan çift PGF2α uygulamalarının elde edilen
embriyoların kalitesi ve sayısı üzerine etkileri araştırıldı. Araştırmada, 5-7 yaşlı Holştayn ırkı, herhangi bir
sağlık ve reprodüktif problemi bulunmayan, siklusları
düzenli seyreden 20 baş Holştayn ırkı inek iki gruba
ayrıldı (n=10). Donörlere östrus siklusunun herhangi
bir gününde CIDR uygulandı. CIDR uygulandıktan sonra
süperovulasyon amacıyla yedinci günden başlayarak
dört gün süreyle 12 saat aralıklarla azalan dozlarda
(80:80 mg, 60:60mg, 40:30 mg, 30:20 mg) toplam 400
mg FSH kas içi yolla uygulandı. Daha sonra corpus
luteumu (CL) lize etmek amacıyla Grup I’de 5. FSH enjeksiyonuyla birlikte tek doz 500 μg Cloprostenol ve
Grup II’de 5. ve 6. FSH enjeksiyonuyla birlikte çift doz
toplam 1000 μg kas içi enjekte edildi. Her iki grupta da
6. FSH enjeksiyonuyla birlikte CIDR uzaklaştırıldı.
Cloprostenol enjeksiyonundan 24 saat sonra günde üç
kez donörlerin östrusları takip edildi ve östrus başlangıcından itibaren 12 saat ara ile üç kez tohumlandı. Embriyolar tohumlamayı takiben yedinci günün sonunda
uterus yıkaması ile toplandı.
Sonuç olarak, iki protokol arasında embriyo sayısı ve
kalitesi açısından fark bulunamamıştır. Ancak donörlere
östrustan önce çift prostaglandin uygulamasının embriyo sayısı ve kalitesi üzerine sayısal olarak olumlu etkisinin olduğu kanaatine varılmıştır

Anahtar Kelimeler

İnek, CIDR, Prostaglandin F2α, Embriyo transferi

The Effect of Double Pgf2α Administrations Applıed to Donor Before Oestrus With Synchronized Cidr on the Number and Qualıty of Obtained Embryos DuringEmbryo Transfer in Cattle

Öz

In this study, the effect of embryo transfer applications
from pre-applied double-PGF2α before the oestrus
period on the number and quality of the embryos in
cattle were investigated. In the study, 20 Holstein cows
aged 5 to 7 years and do not have any health and
reproductive problems and had regular cycles were
divided into two groups (n = 10). CIDR was applied to
donors in any one day of estrous cycle. İn order to
superovulation decreasing FSH doses were
intramuscularly administered after CIDR application by
starting from 7th day with 12-hour intervals for four
days (mg/ 80:80 mg, 60:60 mg, 40:30 mg, 30:20 mg)
which was totally 400 mg. And later, in order to lyses
corpus luteum with 5th FSH injections with a single
dose 500 μg Cloprostenol and with 5th and 6th FSH
injections with a double dose (500 μg+500 μg)
Cloprostenol intramuscularly injected to Group I and
Group II, respectively. In both Groups, CIDR was
removed at the same time with 6th FSH injections.
Donors’ oestrus were followed up after 24 hours of
cloprostenol injection three times a day and from the
beginning of estrus with a 12-hours intervals three
times inseminated. Embryos were collected at the end
of the 7th day following the insemination, by uterus
washing (flush).
In conclusion, no statistically significant differences
between the two protocols was found in terms of
embryo quality and number, but it was concluded that
double-prostaglandin applications to the donors’ before
the oestrus, had positive impact on the quality and
number of embryos.

Anahtar Kelimeler

Cattle, CIDR, Prostaglandin F2α, Embryo transfer

Kaynakça

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