TAVŞANLARDA FARKLI EIMERIA STIEDAI SUŞLARI İLE OLUŞTURULAN DENEYSEL KARACİĞER KOKSİDİYOZUNDA PATOLOJİK VE BİYOKİMYASAL BULGULARIN KARŞILAŞTIRILMASI

Yıl 2018, Cilt: 27 Sayı: 2, 111 - 117, 01.07.2018

Ayhan Atasever Gökhan Seyfi Görkem Ekebaş

Öz

Çalışma, tavşanlarda E. stiedae’nin saha ve standart
suşuyla oluşturulan karaciğer koksidiyozisinde klinik,
biyokimyasal ve patolojik bulguları karşılaştırmak amacıyla yapılmıştır. Çalışmada, 1-1.5 kg, 6-8 haftalık 36
adet yeni Zelanda tavşanı kullanılmıştır. Hayvanlar 3
gruba ayrılmıştır. Birinci grup kontrol tutulurken, sırasıyla ikinci ve üçüncü gruptaki tavşanlara, 1 ml’sinde
50.000 sporlanmış oosit içeren saha ve standart suştan,
1 ml direkt sonda ile ağızdan mideye verilmiştir.
Makroskopik ve histolojik olarak Eimeria stiedae standart suşu inokule edilen üçüncü gruba göre, saha suşu
inokule edilen ikinci grupta daha şiddetli karaciğer
lezyonları belirlenmiştir. Etken inokulasyonunun 20. ve
30. günlerinde; serum gamma glutamiltransferaz (GGT),
alanin aminotransferaz (ALT) ve aspartat
aminotransferaz (AST) enzim aktivitelerinde grup bir
ile grup ikideki tavşanlar arasında istatistiksel önemli
bir fark bulunmamıştır. Buna karşın, etken
inokulasyonunun 20. ve 30. günlerinde, grup birdeki
tavşanlara ait serum GGT, ALT ve AST enzim aktivitelerine göre grup üçteki tavşanların enzim aktivitelerinde
önemli bir artış (p<0.001) belirlenmiştir. Tavşanlarda
E. stiedae’nin saha ve standart suşu ile oluşturulan karaciğer koksidiyozisinde bazı klinik, biyokimyasal ve patolojik değişikliklerin, oluşan enfeksiyonun şiddeti ile
farklılık gösterebileceğinin ortaya konulduğu ilk çalışmadır

Anahtar Kelimeler

Biyokimyasal parametre, Eimeria stiedae, patoloji, tavşan.

Comparison of Pathological and Biochemical Findings in Experimentally Induced the Liver Coccidiosis by Different Types of Eimeria Stiedae Strains in the Rabbits

Öz

The aim of this study was to compare clinical, biochemical and pathological findings of the liver coccidiosis
induced by field and standard strain of the Eimeria
stiedae in the rabbits. Thirty-six New Zealand rabbits
weighing 1.5 kg and aged 6-8 weeks were used. The
rabbits were allocated into three groups, the first of
which was healthy control group. One ml of inoculum of
field strain and standard strain containing 50 000
sporulated E.stiedae oocysts per ml were administered
orally by a catheter to each rabbit in the second group
and third group, respectively. Liver lesions were more
severe in the second group of the Eimeria stiedae field
strain inoculated than the third group in which the
Eimeria stiedae standard strain was inoculated macroscopically and histologically. No statistical difference
was found in serum GGT, ALT and AST enzyme activities between the first and second group at day 20 and
30 after the inoculation. However, at day 20 and 30
after the inoculation, a significant increase (p<0.05) was
found in serum GGT, ALT and AST enzyme activities of
the rabbits in the third group compared to those in
group one. This is the first study to suggest that some
clinical, biochemical and pathological changes in the
liver coccidiosis induced with a field and standard
strain of E. stiedae may vary depending on the severity
of the infection.

Anahtar Kelimeler

Biochemical parameter, Eimeria stiedae, pathology, rabbit.

Kaynakça

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