HPLC-PDA DETERMINATION OF PSEUDO TARAXASTEROL (Ψ-TARAXASTEROL)IN DIFFERENT COUSINIA SPECIES

Yıl 2021, Cilt: 30 Sayı: 1, 17 - 20, 09.04.2021

Leyla Paşayeva Hanifa Fatullayev Şehmus Kılıç Osman Üstün Osman Tugay

https://doi.org/10.34108/eujhs.632581

Öz

Cousinia Cass. is one of the widespread genera of Asteraceae family. There is a large number ofpharmacological activity studies on this genus and it has not yet been examined phytochemically in detail. Pseudo taraxasterol (ψ-taraxasterol) is a pentacyclic triterpene with different pharmacological activity such as antinociceptive, anti-inflammatory. In this report, we aimed to evaluate the ψ-taraxasterolcontent, which isolated from C. stenocephala in seven Cousiniaspecies by high-performance liquid chromatography-photodiode array detector (HPLC-PDA)method. According to quantitative analyses the highest content of ψ-taraxasterol was detected in C. davisiana, C. stenocephala, and C. ramosissima extracts (0.423±0.004, 0.395±0.004 and 0.374±0.000 g/100gextract, respectively). This is the first report on the quantification of pseudo taraxasterol by HPLC-PDA method in this genus, and this result will shed new lights on the advanced biological activity studies on species of this genus.

Anahtar Kelimeler

Asteraceae, Cousinia, HPLC-PDA, pentacyclic triterpene

Destekleyen Kurum

Bilimsel Araştırma Projeleri (BAP) Koordinasyon Birimi

Proje Numarası

THD-2019-8713

Teşekkür

The authors are grateful to the Erciyes University Scientific Research Projects Coordinating Unit (BAP, project number THD-2019-8713) for financial support and Erciyes University Ziya Eren Drug Application and Research Center (ERFARMA) for providing HPLC facility.

FARKLI COUSINIA TÜRLERİNDE PSÖDO TARAKSASTEROL (Ψ-TARAKSASTEROL) BİLEŞİĞİNİN HPLC-PDA İLE MİKTAR TAYİNİ

Öz

Cousinia Cass. cinsi Asteraceae familyasına ait geniş yayılış alanına sahip cinslerden biridir. Bu cins üzerinde yok denecek kadar az farmakolojik etki ve fitokimyasal çalışmalar bulunmaktadır. Psödotaraksasterol (ψ-taraksasterol) antinosiseptif, anti-enflamatuvar aktiviteye sahip pentasikliktriterpen yapısında bileşiktir. Bu çalışmada C. stenocephala bitkisinden izole edilen ψ-taraksasterol bileşiğinin yedi farklı Cousinia türünde HPLC-PDA metodu ile miktar tayini yapılmıştır. Elde edilen sonuçlara gore enyüksek ψ-taraksasterol miktarı C. davisiana, C. stenocephala ve C. ramosissima ekstrelerinde bulunmuştur (sırasıyla, 0.423±0.004, 0.395±0.004 ve 0.374±0.000 g/100gekstre). ψ-taraksasterol bileşiği bu çalışmada HPLC-PDA metodu ile bu cinste ilk defa tayin edilmiş ve bu sonuçların cinsin farklı türlerinin çeşitli biyolojik aktivite araştırmalarında faydalı olacağı düşünülmektedir.

Anahtar Kelimeler

Asteraceae, Cousinia, HPLC-PDA, pentasiklik triterpene

Proje Numarası

THD-2019-8713

Kaynakça

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