Theranekron®'un sağlıklı ve iskemi-reperfüzyon hasarı oluşturulan rat ovaryumunda etkisi

Öz

Bu çalışmada sağlıklı ve iskemi-reperfüzyon yaralanmalı sıçan modelinde Theranekron®'un yumurtalıklar üzerindeki etkisi değerlendirildi. Otuz sekiz adet nulligravida dişi Wistar Albino rat aşağıdaki gibi gruplara ayrıldı; Grup 1: kontrol, Grup 2: Theranekron® (tek doz 0,3 mg/kg, intraperitoneal, 3 saat) Grup 3: iskemi-reperfüzyon ve Grup 4: iskemi-reperfüzyon+ Theranekron® (tek doz 0,3 mg/ kg, intraperitoneal, 3 saat). Her grup biyokimyasal ve histolojik olarak analiz edildi. Grup 1'e göre Grup 2'de atretik folikül ve interstisyel hücre sayısında anlamlı artış vardı. Ayrıca nitrit, nitrat ve malondialdehit (MDA) seviyeleri Grup 2'de anlamlı artış gösterdi. Grup 3'te yoğun hiperemi ve kanamalar mevcuttu. Grup 2'ye göre korteksi çevreleyen ve korpus luteumdaki foliküllerde ve atretik folikül sayısında belirgin artış oldu. Grup 3'teki nitrit ve nitrat seviyeleri Grup 2 ile benzer, ancak MDA seviyeleri Grup 2'den düşüktü. Grup 4, Grup 2 ve Grup 3 ile karşılaştırıldığında interstisyel hücrelerde çok hafif azalma gösterdi ve ayrıca daha az atretik folikül gözlendi. Bu grupta MDA düzeyleri Grup 2'ye göre azalmış, kontrol ve Grup 3'e göre yükselmiştir. Sonuçlara dayanarak, Theranekron®'un sağlam yumurtalıklar üzerinde bazı dejeneratif etkilere sahip olabileceğini ve yumurtalıklarda iskemi reperfüzyon hasarı üzerinde iyileştirici etkileri olabileceğini tahmin edebiliriz. Ancak Theranekron®'un sıçan yumurtalıklarında iskemi reperfüzyon hasarı üzerine optimum iyileştirici dozunu belirlemek için daha ileri çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

• İskemi-reperfüzyon
• Malondialdehit
• Nitrat/Nitrit
• Theranekron®
• Rat

The effect of Theranekron® in intact and with ischemia-reperfusion injury rat ovary

Öz

In this study, the effect of Theranekron® on ovaries were evaluated in healthy and ischemia- reperfusion injury rat model. Thirty-eight female, nulligravida Wistar Albino rats were divided into groups as follows; Group 1: control, Group 2: Theranekron® (single dose of 0,3 mg/kg, intraperitoneal, for 3 hours) Group 3: ischemia-reperfusion and Group 4: ischemia- reperfusion+ Theranekron® (single dose 0,3 mg/kg, intraperitoneal, for 3 hours). Each groups were analyzed biochemically and histologically. As compared with Group 1, there was a significant increase in the number of atretic follicles and interstitial cells in Group 2. Also, nitrite, nitrate, and malondialdehyde (MDA) levels increased significantly in Group 2. Group 3 represented intense hyperemia and hemorrhages on follicles surrounding the cortex and in the corpus luteum and marked increase in the number of atretic follicles, compared to Group 2. Nitrite and nitrate levels in Group 3 were similar to those in Group 2, but MDA levels were lower than those in Group 2. Group 4 showed very slight decrease in interstitial cells compared with Group 2 and Group 3 and also fewer atretic follicles were observed. In this group, MDA levels decreased compared to Group 2 and increased compared to control and Group 3. Based on the results, we may speculate that Theranekron® may have some degenerative effects on intact ovaries, and may have ameliorationg effects on ischemia reperfusion injury in ovaries. But, further studies are needed to determine the optimum remedial dose of Theranekron® on ischemia reperfusion injury in rat ovaries.

Anahtar Kelimeler

• Ischemia-reperfusion
• Malondialdehyde
• Nitrate/Nitrite
• Rat
• Theranekron®

Kaynakça

1. Abdel-Hafez HZ, Mohamed E-EM, Abd-Elghany AA. (2010) Tissue and blood superoxide dismutase activity and malondialdehyde level in leprosy. JEADV. 24(6),704–708. doi:10.1111/j.1468-3083.2009.03496.x
2. Adib-Hashemi F, Farahmand F, Hesari SF, Rezakhaniha B, Fallah E, Fayyaz FA, Dadpay M. (2016) Retraction note: Anti-inflammatory and protective investigations on the effects of Theranekron® "an alcoholic extract of the Tarantula cubensis" on wound healing of peritoneal in the rat: an in vivo comparative study. Diagnostic Pathology. 11(1), 118. doi: 10.1186/s13000-016-0575-2.
3. Akalın F. (2014) The protective effect of sivelestat and edaravone on ischemia-reperfusion injury in rat ovary. Master thesis, Dokuz Eylül University, Izmir.
4. Birincioğlu M. (2004) Introduction to ischemia-reperfusion techniques. Master thesis, Adnan Menderes University, Aydın.
5. Bostancı MS, Bakacak M, İnanc F, Yaylalı A, Serin S, Attar R, Yildirim ÖK, Yildirm G. (2016) The protective effect of G-CSF on experimental ischemia/reperfusion injury in rat ovary. Archives of Gynecology and Obstetrics. 293(4), 789-95. doi: 10.1007/s00404-015-3878-8.
6. Cadirci E, Oral A, Odabasoglu F, Kilic C, Coskun K, Halici Z, Suleyman H, Keles ON, Unal B. (2010) Atorvastatin reduces tissue damage in rat ovaries subjected to torsion and detorsion: biochemical and histopathologic evaluation. Naunyn-Schmiedebergs Archives of Pharmacology. 381(5), 455-66. doi: 10.1007/s00210-010-0504-y.
7. Dantas F, Rampes H. (2000) Do homeopathic medicines provoke adverse effects? A systematic review. British Homeopathic Journal. 89(1), 35-38. doi: 10.1054/homp.1999.0378.
8. Demirci İ, Delibaşı T, Kayahan İK, Demirci EBS. (2004) Is Acute Ischemic Cerebrovascular Event Reperfusion Damaged Developments. Toward Molecular Mechanisms. The Journal of Medical Investigations. 2(3), 53-56.

9. Denk H, Künzele H, Plenk H, Rüschoff J, Sellner W. (1989) Romeis Microscopische Technic. 17, Neubearbeitete Auflage, Urban and Schwarzenberg, München, p.439-450.
10. Dolapcioglu K, Dogruer G, Ozsoy S, Ergun Y, Ciftci S, Karapinar OS, Aslan E. (2013) Theranekron® for treatment of endometriosis in a rat model compared with medroxyprogesterone acetate and leuprolide acetate. European Journal of Obstetrics Gynecology and Reproductive Biology. 170(1), 206-210. doi: 10.1016/j.ejogrb.2013.05.026.
11. Ergun Y, Koc A, Dolapcioglu Y, Akaydin Y, Dogruer G, Kontas T, Kozlu T, Aslan E. (2010) The protective effect of erythropoietin and dimethylsulfoxide on ischemia-reperfusion injury in rat ovary. European Journal of Obstetrics Gynecology and Reproductive Biology. 152, 186-190. doi: 10.1016/j.ejogrb.2010.05.025.
12. Gönül R, Koenhemsi L, Aydın H, Gulyasar T, Demırcan-Yardibi H, Or E, Hosturk G, Uysal A, Barutçu B. (2015) Effects of Tarantula Cubensis Extract on Electrocardiographic Status and Trace Elements in Sheep. Journal of The Faculty of Veterinary Medicine Istanbul University. 41(1), 79-83. doi: 10.16988/iuvfd.2015.25554
13. Gürbulak K, Akçay A, Gümüşsoy KS, Sıst B, Steıner S, Abay M, Canooğlu E, Bekyürek T. (2014) Investigation of the Efficacy of Tarantula cubensis Extract (Theranekron® D6) in the Treatment of Subclinical and Clinical Mastitis in Dairy Cows. Turkısh Journal of Veterınary and Animal Sciences. 38, 712-718. doi: 10.3906/vet-1405-101
14. Hascalik S, Celik O, Turkoz Y, Mizrak B. (2005) Clip Turcica: A new apparatus for experimental ovarian ischemia and reperfusion model in rats. Fertility and Sterility. 84(1), 219-220. doi: 10.1016/j.fertnstert.2005.03.024.
15. Hirshfield AN. (1989) Rescue of atretic follicles in vitro and in vivo. Biology of Reproduction. 40(1), 181-190. doi: 10.1095/biolreprod40.1.181.
16. Kaçar C, Zonturlu AK, Oral H, Yıldız S, Arı UÇ. (2007) The effects of Theranekron® application on uterus involution and vaginal efluence in cows early puerperal period. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 13(1), 11-15. doi:10.9775/kvfd.2006.36-A.
17. Kavak SB, Kavak E, Kurkut B, İlhan R, Başpınar M. (2014) The Importance of Clinical, Radiologic and Laboratory Parameters in the Diagnosis of Ovarian Torsion: Retrospective Study. Medical Journal of Istanbul Kanuni Sultan Süleyman. 6(1), 20-22. DOI: 10.5222/IKSST.2014.020.
18. Lebovic DI, Kir M, Casey CL. (2004) Peroxisome proliferator-activated receptor-gamma induces regression of endometrial explants in a rat model of endometriosis. Fertility and Sterility. 82, 1008–1013. doi: 10.1016/j.fertnstert.2004.02.148.
19. Lotfollahzadeh S, Alizadeh MR, Mohri M, Mokhber Dezfouli MR. (2012) The therapeutic effect of Tarentula cubensis extract (Theranekron®) in foot-and-mouth disease in cattle: a randomised trial in an endemic setting. Homeopathy. 101(3), 159-164. doi: 10.1016/j.homp.2012.05.008.
20. Oelsner G, Bider D, Goldenberg M, Admon D, Mashiach S. (1993) Long-Term Follow-up of the Twisted Ischemic Adnexa Managed by Detorsion. Fertility and Sterility. 60(6), 976-979. doi: 10.1016/s0015-0282(16)56395-x.
21. Ohkawa H, Ohishi N, Yagi K. (1979) Assay for lipit peroxides in animal tissues by thiobarbituric acid reactio. Analytical Biochemistry. 95(2), 351-358. doi: 10.1016/0003-2697(79)90738-3.
22. Oryan A, Moshiri A, Raayat AR. (2012) Novel application of Theranekron® enhanced the structural and functional performance of the tenotomized tendon in rabbits. Cells Tissues Organs. 196(5), 442-455. doi: 10.1159/000337860.
23. Parashar U, Uppal T. (2011) Ovarian torsion is an infrequent, but important, cause of acute abdomino-pelvic pain in women. O&G Magazine. 13(1), 34-35.
24. Richardson-Boedler C. (2002) The Brown spider Loxosceles laeta: source of the remedy Tarentula cubensis. Homeopathy. 91(3), 166-170. doi: 10.1054/homp.2002.0029.
25. Şahinarslan N. (2009) Investigation of the effects of nitric oxide on the development of the rat ovary in the light and electron microscopic level. Master thesis, Istanbul University, İstanbul.
26. Stampa S. (1986) A field trial comparing the efficacy of sulphamonomethoxine, penicillin and tarantula poison in the treatment of pododermatitis circumspecta of cattle. Journal of the South African Veterinary Association. 57(2), 91-93.
27. Turgut G, Enli Y, Kaptanoğlu B, Turgut S, Genç O. (2006) Changes in the levels of MDA and GSH in mice serum, liver and spleen after aluminum administration. Eastern Journal of Medicine. 11, 7-12.
28. Turkoz Y, Celik O, Hascalik S, Cigremis Y, Hascalik M, Mizrak B, Yologlu S. (2004) Melatonin reduces torsion-detorsion injury in rat ovary: biochemical and histopathologic evaluation. Journal of Pineal Research. 37(2), 137-141. doi: 10.1111/j.1600-079X.2004.00146.x.