SEROEPIDEMIOLOGY OF PESTIVIRUS INFECTION IN HONAMLI GOAT BREED

Öz

Pestiviruses, which cause multisystemic infections and high economic losses in livestock, have a wide host spectrum. Since these viruses are not host-specific, interspecies transmission is important in terms of epidemiological prevalence. Due to pestivirus infections in goats, abortions in pregnant goats, anomalous litter births and yield losses may occur. In this study, it was aimed to determine the seroprevalence of pestivirus infection in Honamlı goats farmed in Burdur region. In this direction, blood serum samples taken from 251 Honamlı goats of pure breed were analyzed by ELISA method. Of the blood serum samples tested, 115 (45.82%) were seropositive. Seropositivity rates were determined between 85.42% and 8% according to sampling regions. The distribution of positivity according to age groups was also determined. It was determined that the difference between seropositivity rates determined on the basis of age groups and gender was statistically insignificant, while the proportional difference determined on the basis of districts was significant. As a result of this study, the presence/prevalence of pestiviruses in Honamlı goat breed was revealed for the first time. This situation showed that pestivirus infections are circulating in the region and may cause economic losses.

Anahtar Kelimeler

• Antibody
• ELISA
• Honamli goat
• Pestivirus

HONAMLI KEÇİ IRKINDA PESTİVİRUS ENFEKSİYONUNUN SEROEPİDEMİYOLOJİSİ

Öz

Çiftlik hayvanlarında multisistemik enfeksiyon tablosu ve yüksek ekonomik kayıplara sebep olan pestiviruslar geniş konakçı spektrumuna sahiptirler. Bu viruslar konakçı spesifik olmadıklarından, türler arası geçiş epidemiyolojik yaygınlık açısından önemlidir. Keçilerde oluşan pestivirus enfeksiyonlarına bağlı olarak gebelerde abortlar, anomalili yavru doğumları ve verim kayıpları meydana gelebilmektedir. Bu araştırmada Burdur yöresinde yetiştiriciliği yapılan Honamlı keçilerinde pestivirus enfeksiyonunun seroprevalansının belirlenmesi amaçlanmıştır. Bu doğrultuda saf ırk özelliğinde 251 Honamlı keçisinden alınan kan serumu örnekleri ELISA yöntemiyle analiz edildi. Test edilen kan serumu numunesinin 115’i (%45,82) seropozitif olarak belirlendi. Örnekleme yapılan yerleşim bölgelerine göre seropozitiflik oranları %85,42-%8 arasında tespit edildi. Ayrıca pozitifliğin yaş gruplarına göre dağılımları da saptandı. Yaş grupları ve cinsiyet bazında belirlenen seropozitiflik oranları arasındaki farklılığın istatistiki açıdan önemsiz, ilçeler bazında belirlenen oransal farklılığın ise önemli olduğu belirlendi. Bu araştırma sonucunda Honamlı keçi ırkında ilk defa pestivirusların varlığı/yaygınlığı ortaya koyulmuştur. Bu durum bölgede pestivirus enfeksiyonlarının sirkülasyonda olduğunu ve ekonomik kayıplara yol açabileceğini göstermiştir.

Anahtar Kelimeler

• Antikor
• ELISA
• Honamlı keçi
• Pestivirus

Kaynakça

1. Abdel-Latif AO, Goyal SM, Chander Y, Abdel-Moneim AS, Tamam SM, Madbouly HM. (2013) Isolation and molecular characterisation of a pestivirus from goats in Egypt. Acta Vet Hung. 61(2),270-280.
2. Albayrak H, Ozan E, Kadi H, Çavuna T, Tamer C (2016). Seroprevalence of pestiviruses in some goat breeds in samsun province. Atatürk Uni Vet Know Derg, 11(1), 1-5.
3. Bauermann, F. V., Ridpath, J. F. (2021). Epidemiology of pestivirus H in Brazil and its control implications. Frontiers in veterinary science, 8, 693041.
4. Broaddus CC, Lamm CG, Kapıl S, Dawson L, Holyoak GR. (2009). Bovine viral diarrhea virus abortion in goats housed with persistently infected cattle. Vet Pathol. 46, 45-53.
5. Campbell E, Mcconville J, Clarke J, Donaghy A, Moyce A, Byrne AW, Guelbenzu‐Gonzalo M (2021). Pestivirus apparent prevalence in sheep and goats in Northern Ireland: a serological survey. Veterinary Record, 188(1), E1.
6. Cirone F, Cirone S, Trerotoli P, Buono R, Ciappetta G, Di Summa A, Pratelli A (2018). Cross sectional study for pestivirus infection in goats in southern Italy. Small Ruminant Research, 166, 12-16.
7. Doğan F, Ataseven VS, Ergün Y (2021). Türkiye’de doğu akdeniz illerindeki sütçü keçi işletmelerinde pestivirus enfeksiyonlarinin seroprevalansı. Etlik Veteriner Mikrobiyoloji Dergisi, 32(1), 45-49.
8. Hasırcıoğlu S, Kale M, Acar A (2009). Investigation of pestivirus infections in aborted sheep and goats in Burdur region. Kafkas Univ Vet Fak Dergisi, 15(2), 163-167, 2009.

9. Houe H (1999). Epidemiological features and economical importance of bovine virus diarrhoea virus (BVDV) infections. Veterinary Microbiology, 64(2-3), 89-107.
10. International Committee of Virus Taxonomy (ICTV). (2022). Https://Talk.İctvonline.Org/İctv-Reports/İctv_Online_Report/Positive-Sense-Rna-Viruses/W/Flaviviridae/361/Genus-Pestivirus Erişim tarihi: 15.12.2022.
11. Liu D (2016). Molecular detection of animal viral pathogens. CRC Press; Boca Raton, FL, USA, P: 211-222, 223-232. Murphy FA, Gibbs JPE, Horzinek MC, Studdert MJ (1989). Veterinary Virology. Third Edition Ed: Murphy FA, Gibbs JPE, Horzinek MC, Studdert MJ; Academic Press, USA, P: 563-569.
12. Nagai M. Aoki H, Sakoda Y, Kozasa T, Tominaga-Teshima K, Mine J, Shirai J (2014). Molecular, biological, and antigenic characterization of a border disease virus isolated from a pig during classical swine fever surveillance in Japan. Journal of Veterinary Diagnostic Investigation, 26(4), 547-552.
13. Newcomer BW, Givens MD (2013). Approved and experimental countermeasures against pestiviral diseases: bovine viral diarrhea, classical swine fever and border disease. Antiviral Research, 100(1), 133-150.
14. Oguzoglu TC, Tan MT, Toplu N, Demir AB, Bilge-Dagalp S, Karaoglu T, Greiser-Wilke I (2009). Border disease virus (bdv) infections of small ruminants in Turkey: A new bdv subgroup? Veterinary Microbiology, 135(3-4), 374-379.
15. Plant JW, Walker KH, Acland HM, Gard GP (1983). Pathology in the ovine foetus caused by an ovine pestivirus. Australian veterinary journal, 60(5), 137-140.
16. Potârniche AV, Czopowicz M, Szaluś-Jordanow O, Moroz A, Mickiewicz M, Witkowski L, Kaba J (2020). Herd-level seroprevalence of pestivirus infection in goat population in Poland. Polish Journal of Veterinary Sciences, 229-233.
17. Ridpath JF (2010). Bovine viral diarrhea virus: global status. Veterinary Clinics: Food Animal Practice, 26(1), 105-121.
18. Riitho V, Strong R, Larska M, Graham SP, Steinbach F (2020). Bovine pestivirus heterogeneity and its potential impact on vaccination and diagnosis. Viruses, 12(10), 1134.
19. Saeed IK (2020). Border disease of sheep and goats in Saudi Arabia. Indian Journal of Microbiology Research, 7(1), 95-8.
20. Schweizer M, Peterhans E. (2014) Pestiviruses. Annu Rev Anim Biosci. 2, 141-163.
21. Shi H, Kan Y, Yao L, Leng C, Tang Q, Ji J, Sun S (2016). Identification of natural infections in sheep/goats with hobi‐like pestiviruses in China. Transboundary and Emerging Diseases, 63(5), 480-484.
22. Thiel HJ, Collett MS, Gould EA, Heinz FX, Houghton M, Meyers G, Purcell RH, Rice CM (2005). Family Flaviviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (Eds.), virus taxonomy. Classification and nomenclature of viruses. 8th Ed. Elsevġer Academic Press, San Diego, P. 981-998.
23. Türkiye İstatistik Kurumu, Hayvansal Üretim İstatistikleri, Haziran 2022, Sayı: 45593 Https://Data.Tuik.Gov.Tr, Erişim tarihi: 21.01.2023.
24. Ural ZE, Nural E (2017). Aydın ve İzmir illerindeki koyun ve keçilerde pestivirus enfeksiyonunun serolojik ve virolojik olarak araştırılması. Harran Üniversitesi Veteriner Fakültesi Dergisi, 6(1), 63-68.
25. Walz PH, Grooms DL, Passler T, Ridpath JF, Tremblay R, Step DL, Givens MD (2010). Control of bovine viral diarrhea virus in ruminants. Journal of Veterinary Internal Medicine, 24(3), 476-486.