Stafilokok'ların Koagulaz ve Clumping Factor Aktivitelerinin Belirlenmesinde Plazma Seçiminin Önemi

Öz

Tavuk orijinli stafilokok suşlarının koagulaz ve clumping factor aktivitelerine çeşitli hayvan plazmalarının (tavşan, tavuk, koyun) etkisi incelendi. Çalışmalarda, tavuklardan izole edilen 126 S.aureus ve 118 S.epidermidis suşu kullanıldı. CFT'de S.aureus suşları tavşan plazması ile % 98, tavuk ve koyun plazmaları ile% 95 pozitif bulundu. S.epidermidis suşlarının % 2.5'i tavşan,% 56'sı tavuk ve % 67'si koyun plazması ile pozitif reaksiyon verdi. Tüp koagulaz testinde (TKT), sırasıyla, % 98,% 63 ve% 67'sini koagule etti. S.epidermidis suşlarında bu oran sırasıyla,% 3,% 14 ve% 16 bulundu.

Anahtar Kelimeler

• Stafilokoklar
• Stafilokokların Koagulaz Factor Aktiviteleri
• Stafilokokların Clumping Factor Aktiviteleri
• Nejat AYDIN
• Hatice AYHAN
• 1992
• Etlik Veteriner Mikrobiyoloji Dergisi

«A study on the importance of plasma selection in the determination of coagulase and clumping factor activities of staphylococci»

Öz

The effect of various plasmas (from rabbit, chicken, sheep) on the coagulase and clumping factor activities of avian staphylococci were used in tests. In CFT, 98 %, 95% and 95% of S.aureus strains gave positive reaction with rabbit, chicken and sheep respectively. In tube coagulase test, 98 %, 63 %, 14 % of S.aureus strains coagulated the plasmas of rabbit, chicken, sheep respectively. These were found as 3 %,14 % and 16 % in S.epidermidis strains.

Kaynakça

1. ABRAMSON, C. (1973) : Staphylococcal Enzymes. In: COHEN, J.O. : staphylococci.Wiley-lntercierıce, New York USA.
2. AKAY, Ö., IZGÜR, M., USLANOGLU, B. ve ERGANIŞ, O. (1988) : Hastalıklı Piliçlerden İzole Edilen S.aureus Suşlarının kültürel, Biyokimyasal ve Biyolojik Karakterlerinin Belirlenmesi Üzerinde Bir Araştırma. A. ü. Vet. Fak. Derg., 34: 294-308.
3. BILGEHAN, H. (1987) : Mikrobiyoloji ve Bağışıklık Bilimi.Bilgehan Basımevi , Bornova, izmir.
4. COOPER, J.E., NEEDHAM, J.R. (1976) : An lnvestigation into the Prevalence of S.aureus on Avian Feet. Vet. Rec., 98: 172-174.
5. DEVRIESE, LA. and OEDING, B. (1976) : Characteristics of S.aureus Strains lsolated from Different Animal Species. Res. Vet. Sci., 21: 284-294.
6. DEVRIESE, L.A., DEVOS, A.H. and DAMME, L.R. (1975) : Quantitative Aspects of the S.aureus flora of Poultry. Poult. Sci., 54 : 95-101.
7. DUTHIE, E.S. (1954) : Evidence of Two from of Staphyloccoccal Coagulase. J. Gen. Microbiol., 10: 427-436.
8. GIBES, P.A., PATTERSON, J.T. and THOMPSON, J.K. (1978) : Characterisation of Poultry Isolates of S.aureus by a New Set of Poultry Phages. J. App. Bacteriol., 44: 387- 400.

9. HAJEK, V. and MARSALEK, E. (1971) : The Differentiation of Pathogenic Staphylococci and an suggestion for Their taxonomic Classification. Zbl. Bak. Parasit 1. (Ogic), 217: 176-182.
10. HARRY, E.G. (1967&) : The Characteristics of S.aureus lsolated Fom Chanes of Staphylococcosis in Poultry. Res. Vet. Sci., 8: 479-489.
11. HARRY, E. G. ( 1976b) : So me Characteristics of S.aureus 1 solated From The S kin and Upper Respiratory Tract of Domesticated and Wild (Feral) Birds. Res. Sci., 8: 490-499.
12. KATO, E. and KUME, T. (1980) : Enterotoxigenicity of Bovine Staphylococci lsolated From California Mastitis Test Positive Milkin Japan. Jap. J. Vet. Res., 28: 75-85.
13. LUKASOVA, J., MICKOVA, V. and PCHALEK, J. (1986) : Characteristics of S.aureus Strains lsolated From Slaughtered Poultry. Zbl. Für. Mikrobiol., 141 : 163-168.
14. LENZIES, R.E. (1977) : Comparison of Coagulase, Deoxyribonuclease (DNase) and Heat-Stable Nuclease Tesıs For ldentification of S.aureus. J. Clin. Pathol., 30: 606-608.
15. ORTH, D.S., CHUGG, L.R. and ADERSON, A.W. (1971) : Comparison of Animal Sera For Suitability in Coagulase Testing. Appl. Microbiol., 21 : 420-425.
16. SMITH, H.W. (1954) : Experimental Staphylococcal lnfections in Chickens. J. Pathol. Bacteriol., 47: 81-87.
17. SUBCOMMITTE ON TAXONOMY OF STAPHYLOCOCCI AND MICROCOCCI (1965) :Recommendations. lntern. Bull . Bacteriol. Nomencl. and Taxon., 15: 109-110.
18. TERZOLO, H.R.and SHIMIZU, A. (1978) : Biological Characters and Bacteriophage Typing for S.aureus From Chicken Staphylococcosis and Commercial Balanced Chicken foodin Argentina. Rev. Aargent. Microbiol., 11: 89-101 .