Biyolojik Meteryalde Değişik Metotlarla Striknin Aranması

Öz

Bu çalışmada ülkemizdeki toksikoloji laboratuvarlarında striknin analizi için kullanılabilecek en çabuk, kolay, güvenilir ve ucuz yöntemin seçilmesi amacıyla ince tabaka kromotografi, kağıt kromotografi, renk testi ve GC/MS yöntemleri karşılaştırmalı olarak çalışıldı.Ince tabaka kromotografi Rf değeri, 0.22, yarı-nieel olarak gerçekleştirilen rekoveri oranı % 78 ve hassasiyet sınırı 0.1 ppm olarak, kağıt kromotografi yöntemi Rf değeri 0.30 ve hassasiyet sınırı 1 ppm olarak, renk testi hassasiyet sınırı 0.5 ı-ıg olarak ve GC/MS sisteminde nice! rekoveri oranı % 88, hassasiyet sınırı ise 1 O ppb olarak belirlendi. Ülkemizdeki toksikoloji laboratuvarlarında bulunabilecek alet ve malzeme ile yapılabilmesi, çabuk, ucuz, kolay ve güvenilir olması nedeniyle ince tabaka kromotografi yönteminin rutin olarak kullanılabileceği kanaatine varılmıştır. Ayrıca GC/MS sistemi de aynı anda birçok maddenin analizinin gerçekleştirilebilmesine olanak sağladığından rutin doping analizlerinde tercih edilebilecek bir metot olarak görünmektedir.

Anahtar Kelimeler

• Striknin
• Biyolojik Maddelerde Striknin Aranması
• Niyazi SAVAŞ
• 1994
• Etlik Veteriner Mikrobiyoloji Dergisi
• Zehir
• Toksikoloji
• Doping

«Detection of strychnine in biological materials by using various techniques»

Öz

In this study, the analysis of strychnine was carried out by using thnilayer chromotography, paper chromotography, colour test and GC/MS system to determine a rapid, easy, reliable and cheap method applicable to to-xicology laboratories. lt was found that the Rf, semi-quantitative recovery and detection limit for thi-lyaer chromotography were 0.22, 78 % and 0.1 ppm, and for paper chromotography Rf and detection limit were 0.30 and 1 ppm respectively. Colour test's detetion limit was 0.5 μg . Ouantitative recovery and detection limit for GC/MS were 78% and 10 ppb respectively. lt is concluded that thin-layer chromotography is applicable to toxicology laboratories, because it is rapid, easy to perform, reliable and applicable with the equipment and glassware present in all toxicology laboratories. In addition, GC/MS system which enables the analysis of many substances at the same time can be preferrev for routine doping analysis.

Kaynakça

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