Zencefil’in Antibakteriyal Etkisi (Antibacterial effect of Zingiber officinale (Ginger))

Yıl 2019, Cilt: 30 Sayı: 1, 44 - 50, 30.06.2019

Çiğdem Güceyü Gülşen Goncagül , Elçin Günaydın , Pınar Akpınar

https://doi.org/10.35864/evmd.556983

Cited By: 1

Öz

Antibiyotiklerin
artan kullanımı mikroorganizmaların direnç kazanmasına neden olmuştur. Bu
durumda özellikle kolay elde edilebilen, daha az yan etkiye sahip bitki
kaynaklı terapötik maddelere ihtiyaç duyulmaktadır.

Zingiber officinale eski çağlardan beri tüm dünyada doğrudan
antimikrobiyal  etkinliğe sahip olması
nedeniyle  bakteriyel enfeksiyonların
tedavisinde kullanılmıştır.

Çalışmamızda Zingiber
officinale bitkisinin Soxhlette hazırlanan etanol  ekstraktlarının, standart  Staphylococcus
aureus (ATCC-25923), Escherichia coli
(ATCC-25922) ve dirençli olduğu bilinen hastalardan izole edilmiş Acinetobacter
baumannii ve Klebsiella pneumoniae etkenlerine karşı   antibakteriyel  aktivitesi ile, günümüzde kullanılan,
ampisilin (AM), sefazolin (CZ), sefuroksim (CXM), meropenem (MEM),kolistin
(CL), ofloksasin (OFX), sulfametoksazol/ trimetoprim (SXT), tetrasiklin (TE),
gentamisinin (GM) etkilerinin  agar
difüzyon yöntemi ile karşılaştırılması amaçlandı.

Bu çalışmada
zencefil (Zingiber officinale) 
ekstraktının tüm test edilmiş bakterilerden Staphylococcus aureus
karşı 5 µL ,10 µL,15 µL ve 20 µL ekstrakt emdirilen diskler çevresinde
sırasıyla 7mm,10mm,11mm,15mm inhibisyon zon çapları ile ekstraktın artan
miktarlarıyla etkisinin fazlalaştığı gözlendi. Escherichia coli’de ise
sadece 20 µL ekstrakt uygulanan disk çevresinde 7mm inhibisyon zon çapı
saptandı. Diğer bakterilerde ise ekstraktın etkinliğinin görüldüğü bir
inhibisyon zon çapı oluşmadı.

Sonuç olarak, Zingiber
officinale’nin yapısındaki bileşiklerin Staphylococcus
aureus  ve Escherichia coli’ye karşı doza bağlı olarak antibakteriyel
etkinliğe sahip olması dolayısıyla ekstraktın MİK ve MBK üzerine çalışmaların
yapılmasının faydalı olacağı düşüncesindeyiz.

Anahtar Kelimeler

Zencefil, Antibiyotik duyarlılığı, Antibakteriyal etki

Kaynakça

• KAYNAKLAR
• 1. Abiramasundari.P, Priya V, Jeyanthi GP, and Gayathri DS, (2011). Evaluation of the antibacterial activity of Cocculus hirsutus. Hygeia Journal for Drugs and Medicines, 3(2): 26-31
• 2. Aboaba O, Efuwape BM, (2001). Antibacterial properties of some Nigerian species. Bio Res Comm. 13:183–188.
• 3. Aghazadeh M, Bialvaei AZ, Aghazadeh M, Kabiri F, Saliani N, Yousefi M, Eslami H, Kafil HS, (2016). Survey of the antibiofilm and antimicrobial effects of Zingiber officinale (in vitro study). Jundishapur J Microbiol. February; 9(2): e30167
• 4. Ahmed SA, Jabbar II, Abdul wahed HE, (2012). Study the antibacterial activity of zingiber officinale roots against some of pathogenic bacteria. Al- Mustansiriya J. Sci Vol. 23, No 3.
• 5. Aiyegoro OA, Okoh AI, (2009). Use of bioactive plant products in combination with standard antibiotics: Implications in antimicrobial chemotherapy. J Med Plant Res. 3:1147–1152.
• 6. Akintobi OA, Onoh CC, Ogele JO, Idowu AA, Ojo OV, Okonko IO, (2013). Antimicrobial activity of Zingiber officinale extract against some selected pathogenic bacteria. Nature and Science 2013;11(1):7-15. (ISSN: 1545-0740). http://www.sciencepub.net/nature.
• 7. Akoachere JF, Ndip RN, Chenwi EB, Ndip LM, Njock TE, Anong DN, (2002). Antibacterial effect of Zingiber officinale and Garcinia kola on respiratory tract pathogens. East African Medical Journal. Nov; 79(11):588-92.
• 8. Al-Saimary IE, Bakr S, Khudaier B, Abass Y, (2006). Efficiency of antibacterial agents extracted from Thymus vulgaris l. (lamiaceae). The Internet Journal of Nutrition and Wellness. 4(1).
• 9. Anbu Jeba Sunilson J, Suraj R, Rejitha G, Anandarajagopal K, Anita Gnana Kumari AV, Promwichit P, (2009). In vitro antimicrobial evaluation of Zingiber officinale, Curcuma longa and Alpinia galanga extract as natural food preservatives. American Journal of Food Technology 4 (5):192:200. ISSN 1557-4571
• 10. Auta KI, Galadima AA, Bassey JU, Olowoniyi OD, Moses OO, Yako AB, (2011). Antimicrobial properties of the ethanolic extracts of Zingiber officinale (Ginger) on Escherichia coli and Pseudomonas aeruginosa. Research Journal of Biological Sciences, 6: 37-39.
• 11. Clinical and Laboratory Standards Institute: Performance Standards for Antimicrobial Susceptibility Testing, Twentieth Informational Supplement, M100-S20, CLSI, Wayne, PA (2010).
• 12. Ekwenye UN, Elegalam,NN, (2005). Antibacterial activity of ginger (Zingiber officinale Roscoe) and garlic (Allium sativum L.) extracts on Esherichia coli and Salmonella typhi. International Journal of Medicine and Advance Science 1 (4): 411-416.
• 13. Gull I, Saeed M, Shaukat H, Aslam SM, Samra ZQ, Athar AM, (2012). Inhibitory effect of Allium sativum and Zingiber officinale extracts on clinically important drug resistant pathogenic bacteria. Ann Clin Microbiol Antimicrob. 11: 8. Published online 2012 Apr 27. DOI: 10.1186/1476-0711-11-8.
• 14. HassasYeganeh S, Doost SRH, Goudarzi M, (2018). Antimicrobial effects of Zingiber officinale extracts against multi-drug resistant Acinetobacter baumannii clinical isolates recovered from hospitalized patients in ICU. Journal of Paramedical Sciences (JPS) Summer Vol 9, 42-48. No3. ISSN 2008-4978.
• 15. Jouda MM, Elbashiti T, Masad A, (2016). The antibacterial effect of some medicinal plant extracts and their synergistic effect with antibiotics . Advances in Life Science and Technology. www.iiste.org ISSN 2224-7181 (Paper) ISSN 2225-062X (Online) Vol.46.
• 16. Jouda MM, Elbashiti T, Masad A, Albayoumi M, (2016). The antibacterial effect of some medicinal plant extracts and their synergistic effect with antibiotics. World Journal Of Pharmacy And Pharmaceutical Sciences, Volume 5, Issue 2, 23-33.
• 17. Karuppiah P, Rajaram S, (2012). Antibacterial effect of Allium sativum cloves and Zingiber officinale rhizomes against multiple-drug resistant clinical pathogens. Asian Pac J Trop Biomed; 2(8): 597-601.
• 18. Khameneh B, Diab R, Ghazvini K, Fazly Bazzaz BS, (2016). Breakthroughs in bacterial resistance mechanisms and the potential ways to combat them. Microb Pathog. 95:1–11.
• 19. Koutsaviti A, Milenković M, Tzakou O, (2011). Antimicrobial activity of the essential oil of Greek endemic Stachys spruneri and its main component, isoabienol. Nat Prod Commun. 6:277–80.
• 20. Marcello S, (2001). The psychopharmacology of herbal medications: plant drugs that alter mind, brain, and behavior. The MIT Press, Cambridge, UK. http://dx.doi.org/10.1021/np0007731
• 21. Mohd Nazri NAA, Ahmat N, Adnan A, Syed Mohamad SA, Syaripah Ruzaina SA, (2011). In vitro antibacterial and radical scavenging activities of Malaysian table salad. African Journal of Biotechnology, 10:5728-5735.
• 22. Njobdi S, Gambo M, Ishaku GA, (2018). Antibacterial activity of Zingiber officinale on Escherichia coli and Staphylococcus aureus. Journal of Advances in Biology & Biotechnology. 19(1): 1-8, Article no.JABB.43534. ISSN: 2394-1081
• 23. O’Hara M, Keifer D, Farrel K, Kemper K, (1998). A review of 12 commonly used medicinal herbs. Archives. Fam. Med. (7)523'536.
• 24. Poeloengan M, (2011). The effect of red ginger (Zingiber officinale Roscoe) extract on the growth of mastitis causing bacterial isolates. African Journal of Microbiology Research Vol. 5(4), pp. 382-389, 18 February. Available online http://www.academicjournals.org/ajmr DOI: 10.5897/AJMR10.776 ISSN 1996-0808 ©2011 Academic Journals
• 25. Rawat S, (2015). Evaluation of synergistic effect of ginger, garlic, tumeric extracts on the antimicrobial activity of drugs against bacterial patogens. International Journal of Biopharmaceutics. 6(2): 60-65. e- ISSN 0976 – 1047. Print ISSN 2229 – 7499. Journal homepage: www.ijbonline.com
• 26. Sable MG, Puttewar TY, Patil RY, (2014). Investigation of antibacterial activity af Allium cepa (onion), Zingiber officinale (ginger). International Journal of Current Reaserch Vol.6, Issue, 09, pp.8768-8778, September. ISSN:0975-833X.
• 27. Shanthi Sree KS, Yasodamma N, (2010). Paramageetham CH, phytochemical screening and ın vitro antibacterial activity of the methanolic leaf extract: Sebastiania chamaelea Müell. The Bioscan, 5:173-175.
• 28. Sibanda T, Okoh AI, (2007). The challenges of overcoming antibiotic resistance: Plant extracts as potential sources of antimicrobial and resistance modifying agents. Afr J Biotechnol. 6:2886–2896.
• 29. SivasothyY, Chong WK, Hamid A, Eldeen İM, Sulaiman SF, Awang K, (2011). Essential oils of Zingiber officinale var. rubrum Theilade and their antibacterial activities. Elsevier Food Chemistry 124 , 514–517. Journal homepage: www.elsevier.com/locate/foodchem
• 30. Stefanello MÉ, Pascoal AC, Salvador MJ, (2011). Essential oils from neotropical Myrtaceae: chemical diversity and biological properties. Chem Biodivers. 8:73–94. doi: 10.1002/cbdv.201000098.
• 31. Taura D W, Lawan S, Gumel S M, Umar S, Sadisu UF, (2014). Antibacterial activity of ethanolic extract of Zingiber officinale and Piper nigrum against some clinical isolates. Communications in Applied Sciences ISSN 2201-7372 Volume 2, Number 1, 52-64.
• 32. Ungvarsky J, (2017). Ginger (Zingiber officinale). Salem Press Encyclopedia,© 2017 by Salem Press, Inc., A Division of EBSCO Information Services, Inc.
• 33. http://lokman-hekim.net/bitkiler/zencefil.asp (Erişim Tarihi:02.03.2019)