Isolation and antimicrobial susceptibility of Streptococcus canis from dogs

Yıl 2021, Cilt: 32 Sayı: 1, 6 - 13, 30.06.2021

Ezgi Şababoğlu Dilek Öztürk Hülya Türütoğlu

https://doi.org/10.35864/evmd.756567

Öz

Streptococcus canis is an important opportunistic pathogen that infects animals and humans. It causes various infections, including streptococcal toxic shock syndrome, otitis externa, arthritis, skin infections, necrotizing fasciitis, and septicemia in dogs and cats. It can also lead to human infections. There are still very few specific studies on the identification and antimicrobial resistance of this pathogen. In this study, the phenotypic and biochemical properties of 12 S. canis isolates obtained from the vaginal discharge of 11 dogs and the open wound of one dog with facial necrosis were determined, and their susceptibility to some antibiotics were investigated. The phenotypic and biochemical properties of 12 isolates, such as esculin hydrolysis, hippurate hydrolysis, synergistic CAMP-like hemolytic activity, brain hearth infusion agar containing 6.5% NaCl, and carbohydrate fermentation were investigated by conventional bacteriological methods. All the isolates were found in Group G according to Lancefield serogrouping. The presence of the species-specific 16S-23S rRNA gene intergenic spacer region (ISR) and sodAint gene was also detected in all the isolates using the polymerase chain reaction (PCR) method. All the isolates were detected to be susceptible to penicillin G, cefoperazone, ampicillin and amoxicillin-clavulanic acid, and resistant to gentamicin and neomycin.

Anahtar Kelimeler

Antimicrobial susceptibility, biochemical properties, species-specific genes, Streptococcus canis

Köpeklerden Streptococcus canis'in izolasyonu ve antimikrobiyal duyarlılığı

Öz

Streptococcus canis, hayvanları ve insanları enfekte eden önemli bir opportunistik patojendir. Köpeklerde ve kedilerde artritis, deri enfeksiyonları, otitis eksterna, septisemi, fasiyal nekroz ve streptokokkal toksik şok sendromu gibi çeşitli enfeksiyonlara neden olmaktadır. Ayrıca insan enfeksiyonlarına da yol açabilmektedir. Bu patojenin identifikasyonu ve antimikrobiyal direncine yönelik çalışmalar hala az sayıdadır. Bu çalışmada, hasta 11 köpeğin vajinal akıntısından ve 1 fasiyal nekrozlu köpeğin açık yarasından izole edilen 12 S. canis izolatının fenotipik ve biyokimyasal özellikleri ile bazı antibiyotiklere duyarlılıkları incelendi. 12 izolatın eskülin hidrolizi, hippurat hidrolizi, sinerjistik CAMP benzeri hemolitik aktivitesi, %6.5 NaCl içeren Brain Heart Infusion agar (BHIA)’da üremesi ve karbonhidrat fermentasyonu gibi fenotipik ve biyokimyasal özellikleri konvansiyonel bakteriyolojik metodlar ile incelendi. Tüm izolatların Lancifield serogruplandırmasına göre G grubunda yer aldığı belirlendi. Ayrıca tüm izolatların polimeraz zincir reaksiyonu ile tür spesifik ISR ve sodAint genlerini taşıdığı saptandı. İzolatların tamamının amoksisilin-klavulanik asit, sefaperazon, ampisilin ve penisilin G’ye duyarlı, gentamisin ve neomisine dirençli olduğu belirlendi.

Anahtar Kelimeler

Antimikrobiyal duyarlılık, biyokimyasal özellikler, Streptococcus canis, tür spesifik genler

Kaynakça

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