Araştırma Makalesi
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Sivas İlinde Çocuk Oyun Parklarında Zoonoz Helmint Türlerinin Araştırılması

Yıl 2021, Cilt: 32 Sayı: 2, 124 - 129, 30.12.2021
https://doi.org/10.35864/evmd.999894

Öz

İnsan toxocariasisi ABD Hastalık Kontrol ve Önleme Merkezi (CDC) tarafından göz ardı edilen önemli paraziter hastalıklar sınıfında sınıflandırılmaktadır. Hastalık çocuklarda erişkinlere oranla daha yaygındır. Çünkü çocuklar ev dışında oynamaları esnasında insan toxocariasis etkenleri olan T. canis ve T. cati yumurtalarına daha fazla maruz kalmaktadır. Bu çalışmanın amacı Sivas ilindeki çocuk oyun parklarında zoonoz helmint türlerinin durumunun mikroskobik ve moleküler yöntemlerle araştırılmasıdır. Bu amaçla Temmuz 2020 ve Temmuz 2021 tarihleri arasında 25 çocuk oyun parkından 84 kum numunesi alınmıştır. Ayrıca çocuk oyun parklarının içinden ve etrafından kedi, köpek ve kızıl tilkiye ait 68 dışkı numunesi de toplanmıştır. Çalışmada kum örneklerinin beşinde Toxocara spp. yumurtası tespit edilmiştir. Mikroskobik incelemede Toxocara spp. yönünden pozitif bulunan üç kum örneğindeki yumurtaların T. cati’ye, ikisinin ise T. canis’e ait olduğu PZR ile ortaya konulmuştur. Çalışmada toplanan dışkı örneklerinden; köpeklere ait bir dışkı örneğinde Toxascaris leonina yumurtası tespit edilmişken, kızıl tilki dışkılarında ise T. canis, Acanthocephala, T. leonina ve Capillaria spp. yumurtaları belirlenmiştir. Kedi dışkı örneklerinde ise herhangi bir parazit yumurtasına rastlanmamıştır. Bu çalışma ile bildiğimiz kadarıyla Sivas ilindeki çocuk oyun parkları zoonotik helmint türleri bakımından ilk kez incelenmiştir.

Destekleyen Kurum

Sivas Cumhuriyet Üniversitesi BAP

Proje Numarası

V-107

Kaynakça

  • Akdemir C. (2010) Visceral larva migrans among children in Kütahya (Turkey) and an evolution of playgrounds for T. canis eggs. Turk J Pediatr. 52, 158-162.
  • Ambarli H, Ertürk A, Soyumert A. (2016) Current status, distribution, and conservation of brown bear (Ursidae) and wild canids (gray wolf, golden jackal, and red fox; Canidae) in Turkey. Turkish Journal of Zoology, 40(6), 944-956.
  • Anderson RC. (2000) Nematode parasites of vertebrates: their development and transmission. Second edition. Cabi Publishing, London.
  • Avcioglu H, Burgu A. (2008) Seasonal prevalence of Toxocara ova in soil samples from public parks in Ankara, Turkey. Vector Borne Zoonotic Dis. 8(3), 345-350.
  • Avcioglu H, Balkaya I. (2011) The relationship of public park accessibility to dogs to the presence of Toxocara species ova in the soil. Vector Borne Zoonotic Dis. 11(2), 177-180.
  • Ayaz E, Yaman M, Gül A. (2003) Prevalence of Toxocara spp. eggs in soil of public parks in Van, Turkey. Indian Vet J. 80, 574-576.
  • Aydenizöz-Özkayhan M. (2006) Soil contamination with ascarid eggs in playgrounds in Kirikkale, Turkey. J Helminthol. 80(1), 15-18.
  • Aydın MF. (2020) Presence of Toxocara spp. and Other Zoonotic parasites Ova in Children's Playground in Karaman, Turkey. Turkiye Parazitol Derg. 44(1), 17-20.
  • Borecka A, Gawor J. (2008) Modification of gDNA extraction from soil for PCR designed for the routine examination of soil samples contaminated with Toxocara spp. eggs. J Helminthol. 82(2), 119-122.
  • Bozkurt Ö, Yildirim A, İnci A. (2012) Kayseri İli Parklarında Bulunan Oyun Alanlarının Askarit Türleri İle Kontaminasyonunun Parazitolojik ve Moleküler Yöntemlerle Araştırılması. Kafkas Univ Vet Fak Derg. 18, A175-180.
  • Bystrianska J, Papajová I, Šoltys J, Sasáková N. (2019) Contamination of sandpits with soil-transmitted helminths eggs in an urban environment. Folia Veterinaria. 63, 60-63.
  • Centers for Disease Control and Prevention. (2020) Erişim adresi: https://www.cdc.gov/parasites/toxocariasis/, Erişim tarihi: 24.08.2021.
  • Despommier D. (2003) Toxocariasis: clinical aspects, epidemiology, medical ecology, and molecular aspects. Clin Microbiol Rev. 16(2), 265-272.
  • Dinkel A, von Nickisch-Rosenegk M, Bilger B, Merli M, Lucius R, Romig T. (1998) Detection of Echinococcus multilocularis in the definitive host: coprodiagnosis by PCR as an alternative to necropsy. J Clin Microbiol. 36(7), 1871-1876.
  • Durant JF, Irenge LM, Fogt-Wyrwas R, Dumont C, Doucet JP, Mignon B, Losson B, Gala JL. (2012) Duplex quantitative real-time PCR assay for the detection and discrimination of the eggs of Toxocara canis and Toxocara cati (Nematoda, Ascaridoidea) in soil and fecal samples. Parasit Vectors, 5(1), 1-9.
  • Fakhri Y, Gasser RB, Rostami A, Fan CK, Ghasemi SM, Javanian M, Bayani M, Armoon B, Moradi B. (2018) Toxocara eggs in public places worldwide-A systematic review and meta-analysis. Environ Pollut. 242, 1467-1475.
  • Güçlü F, Aydenizoz M. (1998) Çocuk parklarındaki kumların kopek ve kedi helminti yumurtaları ile kontaminasyonunun tesbiti. Turkiye Parazitol Derg. 23, 24-27.
  • Gürel FS, Ertuğ S, Okyay P. (2005) Prevalence of Toxocara spp. eggs in Public Parks of the City of Aydın, Turkey. Turkiye Parazitol Derg. 29(3), 177-179.
  • Hofer S, Gloor S, Müller U, Mathis A, Hegglin D, Deplazes P. (2000) High prevalence of Echinococcus multilocularis in urban red foxes (Vulpes vulpes) and voles (Arvicola terrestris) in the city of Zürich, Switzerland. Parasitology. 120(2), 135-142.
  • Kazacos KR. (1983) Improved method for recovering ascarid and other helminth eggs from soil associated with epizootics and during survey studies. Am J Vet Res. 44(5), 896-900.
  • Kleine A, Springer A, Strube C. (2017) Seasonal variation in the prevalence of Toxocara eggs on children’s playgrounds in the city of Hanover, Germany. Parasit Vectors. 10(1), 1-8.
  • Li MW, Lin RQ, Chen HH. (2007) PCR tools for the verification of the specific identity of ascaridoid nematodes from dogs and cats. Mol Cell Probes. 21(5-6): 349-354.
  • Nooraldeen K. (2015) Contamination of public squares and parks with parasites in Erbil city, Iraq. Ann Agric Environ Med. 22(3).
  • Oge S, Oge H. (2000) Prevalence of Toxocara spp. eggs in the soil of public parks in Ankara, Turkey. Dtsch Tierarztl Wochenschr. 107(2), 72-75.
  • Overgaauw PAM. (1997) Aspects of Toxocara epidemiology: human Toxocarosis. Crit Rev Microbiol. 23(3), 215-231.
  • Overgaauw PA, van Knapen F. (2013) Veterinary and public health aspects of Toxocara spp. Vet Parasitol. 193(4), 398-403.
  • Robertson ID, Thompson RC. (2002) Enteric parasitic zoonoses of domesticated dogs and cats. Microbes Infect. 4(8), 867-873.
  • Sadowska N, Tomza-Marciniak A, Juszczak M. (2019) Soil contamination with geohelminths in children’s play areas in Szczecin, Poland. Ann Parasitol. 65(1), 65-70.
  • Smith H, Noordin R. (2006) Diagnostic limitations and future trends in serodiagnosis of human toxocariasis. Holland CV, Smith HV. eds. Toxocara the enigmatic parasite, CABI Publishing, Cambridge. 89-112.
  • Taylor MA, Coop RL, Wall RL. (2016) Veterinary parasitology, Fourth edition. Blackwell Publishing, Oxford.
  • Toparlak M, Gargili A, Tüzer E, Keleş V, Esatgil MU, Çetinkaya H. (2002) Contamination of children's playground sandpits with Toxocara eggs in İstanbul, Turkey. Turk J Vet Anim Sci. 26(2), 317-320.
  • Traversa D, di Regalbono AF, Di Cesare A, La Torre F, Drake J, Pietrobelli M. (2014) Environmental contamination by canine geohelminths. Parasit Vectors. 7(1), 1-9.
  • Uga S, Matsuo J, Kimura D, Rai SK, Koshino Y, Igarashi K. (2000) Differentiation of Toxocara canis and T. cati eggs by light and scanning electron microscopy. Vet Parasitol. 2000, 92, 287-294.
  • Zajac AM, Conboy GA, Greiner EC, Smith SA, Snowden KF eds. (2012) Veterinary clinical parasitology. Eighth edition. John Wiley and Sons.

Investigation of Zoonotic Helminths in Children Playgrounds in Sivas Province

Yıl 2021, Cilt: 32 Sayı: 2, 124 - 129, 30.12.2021
https://doi.org/10.35864/evmd.999894

Öz

Human toxocariasis is categorized as a neglected parasitic disease by the Center for Disease Control and Prevention. The disease is more prevalent in children than adults because children are more frequently exposed to the eggs of T. canis and T. cati which are the etiological agents of human toxocariasis during playing outside. The aim of this study was to research the presence of helminth species in children playgrounds in Sivas using microscopic and molecular techniques. For this purpose, 84 sand samples were taken from 25 children playgrounds from July 2020 to July 2021. Moreover, 68 stool samples that belong to cats, dogs, and red foxes were collected from and around children playgrounds. Toxocara spp. eggs were found in five sand samples. According to PCR results, T. cati was detected in three sand samples while T. canis was found in two sand samples. Toxascaris leonina was detected only in one dog stool sample, whereas no parasite species were found in cat stool samples. The eggs of Toxocara canis, Acanthocephala, T. leonina, Capillaria spp. were found in red fox stool samples. To the best of our knowledge, children playgrounds were researched for the first time in terms of zoonotic helminth species in Sivas.

Proje Numarası

V-107

Kaynakça

  • Akdemir C. (2010) Visceral larva migrans among children in Kütahya (Turkey) and an evolution of playgrounds for T. canis eggs. Turk J Pediatr. 52, 158-162.
  • Ambarli H, Ertürk A, Soyumert A. (2016) Current status, distribution, and conservation of brown bear (Ursidae) and wild canids (gray wolf, golden jackal, and red fox; Canidae) in Turkey. Turkish Journal of Zoology, 40(6), 944-956.
  • Anderson RC. (2000) Nematode parasites of vertebrates: their development and transmission. Second edition. Cabi Publishing, London.
  • Avcioglu H, Burgu A. (2008) Seasonal prevalence of Toxocara ova in soil samples from public parks in Ankara, Turkey. Vector Borne Zoonotic Dis. 8(3), 345-350.
  • Avcioglu H, Balkaya I. (2011) The relationship of public park accessibility to dogs to the presence of Toxocara species ova in the soil. Vector Borne Zoonotic Dis. 11(2), 177-180.
  • Ayaz E, Yaman M, Gül A. (2003) Prevalence of Toxocara spp. eggs in soil of public parks in Van, Turkey. Indian Vet J. 80, 574-576.
  • Aydenizöz-Özkayhan M. (2006) Soil contamination with ascarid eggs in playgrounds in Kirikkale, Turkey. J Helminthol. 80(1), 15-18.
  • Aydın MF. (2020) Presence of Toxocara spp. and Other Zoonotic parasites Ova in Children's Playground in Karaman, Turkey. Turkiye Parazitol Derg. 44(1), 17-20.
  • Borecka A, Gawor J. (2008) Modification of gDNA extraction from soil for PCR designed for the routine examination of soil samples contaminated with Toxocara spp. eggs. J Helminthol. 82(2), 119-122.
  • Bozkurt Ö, Yildirim A, İnci A. (2012) Kayseri İli Parklarında Bulunan Oyun Alanlarının Askarit Türleri İle Kontaminasyonunun Parazitolojik ve Moleküler Yöntemlerle Araştırılması. Kafkas Univ Vet Fak Derg. 18, A175-180.
  • Bystrianska J, Papajová I, Šoltys J, Sasáková N. (2019) Contamination of sandpits with soil-transmitted helminths eggs in an urban environment. Folia Veterinaria. 63, 60-63.
  • Centers for Disease Control and Prevention. (2020) Erişim adresi: https://www.cdc.gov/parasites/toxocariasis/, Erişim tarihi: 24.08.2021.
  • Despommier D. (2003) Toxocariasis: clinical aspects, epidemiology, medical ecology, and molecular aspects. Clin Microbiol Rev. 16(2), 265-272.
  • Dinkel A, von Nickisch-Rosenegk M, Bilger B, Merli M, Lucius R, Romig T. (1998) Detection of Echinococcus multilocularis in the definitive host: coprodiagnosis by PCR as an alternative to necropsy. J Clin Microbiol. 36(7), 1871-1876.
  • Durant JF, Irenge LM, Fogt-Wyrwas R, Dumont C, Doucet JP, Mignon B, Losson B, Gala JL. (2012) Duplex quantitative real-time PCR assay for the detection and discrimination of the eggs of Toxocara canis and Toxocara cati (Nematoda, Ascaridoidea) in soil and fecal samples. Parasit Vectors, 5(1), 1-9.
  • Fakhri Y, Gasser RB, Rostami A, Fan CK, Ghasemi SM, Javanian M, Bayani M, Armoon B, Moradi B. (2018) Toxocara eggs in public places worldwide-A systematic review and meta-analysis. Environ Pollut. 242, 1467-1475.
  • Güçlü F, Aydenizoz M. (1998) Çocuk parklarındaki kumların kopek ve kedi helminti yumurtaları ile kontaminasyonunun tesbiti. Turkiye Parazitol Derg. 23, 24-27.
  • Gürel FS, Ertuğ S, Okyay P. (2005) Prevalence of Toxocara spp. eggs in Public Parks of the City of Aydın, Turkey. Turkiye Parazitol Derg. 29(3), 177-179.
  • Hofer S, Gloor S, Müller U, Mathis A, Hegglin D, Deplazes P. (2000) High prevalence of Echinococcus multilocularis in urban red foxes (Vulpes vulpes) and voles (Arvicola terrestris) in the city of Zürich, Switzerland. Parasitology. 120(2), 135-142.
  • Kazacos KR. (1983) Improved method for recovering ascarid and other helminth eggs from soil associated with epizootics and during survey studies. Am J Vet Res. 44(5), 896-900.
  • Kleine A, Springer A, Strube C. (2017) Seasonal variation in the prevalence of Toxocara eggs on children’s playgrounds in the city of Hanover, Germany. Parasit Vectors. 10(1), 1-8.
  • Li MW, Lin RQ, Chen HH. (2007) PCR tools for the verification of the specific identity of ascaridoid nematodes from dogs and cats. Mol Cell Probes. 21(5-6): 349-354.
  • Nooraldeen K. (2015) Contamination of public squares and parks with parasites in Erbil city, Iraq. Ann Agric Environ Med. 22(3).
  • Oge S, Oge H. (2000) Prevalence of Toxocara spp. eggs in the soil of public parks in Ankara, Turkey. Dtsch Tierarztl Wochenschr. 107(2), 72-75.
  • Overgaauw PAM. (1997) Aspects of Toxocara epidemiology: human Toxocarosis. Crit Rev Microbiol. 23(3), 215-231.
  • Overgaauw PA, van Knapen F. (2013) Veterinary and public health aspects of Toxocara spp. Vet Parasitol. 193(4), 398-403.
  • Robertson ID, Thompson RC. (2002) Enteric parasitic zoonoses of domesticated dogs and cats. Microbes Infect. 4(8), 867-873.
  • Sadowska N, Tomza-Marciniak A, Juszczak M. (2019) Soil contamination with geohelminths in children’s play areas in Szczecin, Poland. Ann Parasitol. 65(1), 65-70.
  • Smith H, Noordin R. (2006) Diagnostic limitations and future trends in serodiagnosis of human toxocariasis. Holland CV, Smith HV. eds. Toxocara the enigmatic parasite, CABI Publishing, Cambridge. 89-112.
  • Taylor MA, Coop RL, Wall RL. (2016) Veterinary parasitology, Fourth edition. Blackwell Publishing, Oxford.
  • Toparlak M, Gargili A, Tüzer E, Keleş V, Esatgil MU, Çetinkaya H. (2002) Contamination of children's playground sandpits with Toxocara eggs in İstanbul, Turkey. Turk J Vet Anim Sci. 26(2), 317-320.
  • Traversa D, di Regalbono AF, Di Cesare A, La Torre F, Drake J, Pietrobelli M. (2014) Environmental contamination by canine geohelminths. Parasit Vectors. 7(1), 1-9.
  • Uga S, Matsuo J, Kimura D, Rai SK, Koshino Y, Igarashi K. (2000) Differentiation of Toxocara canis and T. cati eggs by light and scanning electron microscopy. Vet Parasitol. 2000, 92, 287-294.
  • Zajac AM, Conboy GA, Greiner EC, Smith SA, Snowden KF eds. (2012) Veterinary clinical parasitology. Eighth edition. John Wiley and Sons.
Toplam 34 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Bilimleri
Bölüm Araştırma Makaleleri
Yazarlar

Ufuk Erol 0000-0002-6766-1335

Kürşat Altay 0000-0002-5288-1239

Ömer Faruk Şahin 0000-0002-3230-504X

Osman Furkan Urhan 0000-0002-2834-3051

Proje Numarası V-107
Erken Görünüm Tarihi 30 Aralık 2021
Yayımlanma Tarihi 30 Aralık 2021
Gönderilme Tarihi 23 Eylül 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 32 Sayı: 2

Kaynak Göster

APA Erol, U., Altay, K., Şahin, Ö. F., Urhan, O. F. (2021). Investigation of Zoonotic Helminths in Children Playgrounds in Sivas Province. Etlik Veteriner Mikrobiyoloji Dergisi, 32(2), 124-129. https://doi.org/10.35864/evmd.999894


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