Do CMT, SCC, and Bacteriological isolation overlap in subclinical mastitis cases of Anatolian buffaloes?

Yıl 2023, Cilt: 34 Sayı: 1, 1 - 10, 20.06.2023

Gülşen Goncagül , Elçin Günaydın , Anastasia Lisuzzo , Enrico Fiore , Yavuz Çokal , Melis Zeybek

https://doi.org/10.35864/evmd.1274672

Öz

Abstract
Subclinical mastitis is the most important and costly disease in the dairy sector. In this study, it was aimed to compare the results of bacteriological examination with those of California Mastitis Test (CMT) and Somatic Cell Count (SCC)in the milk samples collected from Anatolian buffaloes with no clinical signs of mastitis. For this purpose, 96 milk samples were collected from 24 Anatolian buffaloes of each quarter. All milk samples were examined for the presence of mastitic pathogens by bacteriology regardless of SCC values and CMT scores. A total of 103 isolates were recovered from the infected quarters. The first three frequently isolated mastitic pathogens were determined to be E. coli, S. agalactiae, and S. aureus with the rate of 31.07%, 22.33%, and 21.36%, respectively. According to the bacteriology results, threshold value for SCC was accepted as ≥78.000 cells/ml. The correlation value between CMT and SCC, CMT and bacteriology, and SCC and bacteriology was found as 0.737, 0.845 and 0.872, respectively, and the mean of inter-item correlation was determined 0.818. These results showed that the test results were highly correlated with each other. The results of the ROC analysis of the cut-of-value of the SCC test for this study chosen as 78.000 cells/ml supported the results obtained from the reliability analysis with sensitivity 85% and 1-specifity 100%. To sum up, a combination of CMT, SCC, and bacteriological investigation provides benefits in detecting mastitis early and avoiding misdiagnosis, allowing for timely action and treatment.
Keywords: Anatolian Buffalo, CMT, SCC, bacteriology

Anahtar Kelimeler

Anatolian Buffalo, CMT, SCC, bacteriology

Destekleyen Kurum

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Proje Numarası

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Teşekkür

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Anadolu mandalarının subklinik mastitis vakalarında CMT, SCC ve Bakteriyolojik izolasyon örtüşüyor mu? Özet

Öz

Özet
Subklinik mastitis, süt sektöründe en önemli ve maliyetli hastalıktır. Bu çalışmada, klinik belirti göstermeyen Anadolu süt mandalarından toplanan süt örneklerinde bakteriyolojik inceleme sonuçları ile California Mastitis Testi (CMT) ve Somatik Hücre Sayısı (SCS) sonuçlarının karşılaştırılması amaçlandı. Bu amaçla, 24 Anadolu süt mandasınının her meme lobundan olmak üzere toplam 96 süt örneği alındı. SCC değerleri ve CMT skorlarına bakılmaksızın, tüm süt örnekleri mastitik patojenlerin varlığı açısından bakteriyolojik olarak incelendi. Enfekte loblardan toplam 103 izolat elde edildi. İlk üç en sık izole edilen mastitik patojenlerin sırasıyla %31.07, %22.33 ve %21.36 oranlarıyla E. coli, S. agalactiae ve S. aureus olduğu belirlendi. Bakteriyoloji sonuçlarına göre, SCC için eşik değer ≥78.000 hücre/ml olarak kabul edildi. CMT ve SCC, CMT ve bakteriyoloji, SCC ve bakteriyoloji arasındaki korelasyon değeri sırasıyla 0.737, 0.845 ve 0.872 olarak bulundu ve madde içi korelasyon ortalaması 0.818 olarak belirlendi. Bu sonuçlar, test sonuçlarının birbirleriyle yüksek derecede ilişkili olduğunu gösterdi. Bu çalışma için seçilen SCC testi kesim değeri için ROC analizi sonuçları, duyarlılık %85 ve 1-özgüllük %100 ile güvenilirlik analizinden elde edilen sonuçları destekledi. Sonuç olarak, CMT, SCC ve bakteriyolojik inceleme kombinasyonu, mastitisin erken teşhis edilmesine ve yanlış tanıdan kaçınılarak zamanında müdahale ve tedavi sağlanmasına olanak tanır.
Anahtar kelimeler: Anadolu süt mandası, CMT, SCC, bakteriyoloji

Anahtar Kelimeler

Anadolu süt mandası, CMT, SCC, bakteriyoloji

Proje Numarası

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Kaynakça

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