Deney Hayvanlarında Probiyotikli Yem Kullanımının Bağırsak Mikrobiyotasına Etkisi

Yıl 2024, Cilt: 35 Sayı: 1, 58 - 69, 04.07.2024

Naim Deniz Ayaz Tayfun İde Aşkın Nur Derinöz Erdoğan Muammer Göncüoğlu

https://doi.org/10.35864/evmd.1343536

Öz

Bilimsel çalışmalarda laboratuvar hayvanı kullanımı büyük önem taşımakta ve önemli bir yer tutmaktadır. In-vivo çalışmalarda araştırılanın kesin olarak etkinliğini ortaya koyabilmek için deneyde kullanılan hayvanların ırkı, türü ve yaşının yanı sıra çevre koşulları gibi pek çok koşul kontrol ve test gruplarında bir örnek hale getirilmektedir. Ancak bu deneylerde gastrointestinal sistemin işlevi ve bütünlüğünde, bağışıklık homeostazının korunmasında ve konak enerji metabolizmasında önemli bir rol oynayan bağırsak mikrobiyotasının kullanılan deney hayvanlarında farklılıklar gösterebileceği bu durumun ise çalışma sonuçlarını olumsuz etkileyebileceği çoğu zaman gözden kaçmaktadır. Bu çalışmada, bilimsel çalışmalar öncesi adaptasyon periyodunda kullanılmak üzere laboratuvar hayvanlarının (rat ve fare) bağırsak mikrobiyotalarının senkronizasyonunda kullanım potansiyeli olan probiyotik içerikli yemlerin üretimi ve bağırsak mikrobiyotası üzerine etkinliğinin araştırılması amaçlanmıştır. Analizler neticesinde üretilen sıvı yemlerin muhafaza süresi sonunda toplam aerob genel canlı sayısı ve laktik asit bakteri sayısının (L. acidophilus ve L. plantarum) 109 kob/ml ulaştığı buna karşın koliform bakteri, maya-küf ile diğer patojen mikroorganizmalara rastlanmamıştır. Mikrobiyota analizleri neticesinde ise hem fare hem de ratlarda 10 günlük probiyotikli yem uygulamasının kontrol grupları ile 0. gündeki test gruplarına göre bağırsak mikrobiyotası kompozisyonu üzerine etkili olduğu ortaya konmuştur. Günümüze kadar yapılan literatür incelemelerinde Türkiye’de bu kapsamda bir çalışma olmadığı belirlenmiştir. Bu bağlamda in-vivo çalışmalar öncesinde deney hayvanlarının bu çalışma kapsamında üretilen probiyotik içerikli yemler ile beslenmesinin hayvanların bağırsak mikrobiyotasının geliştirilmesine katkı sunacağı sonucuna varılmıştır.

Anahtar Kelimeler

Laboratuvar hayvanları, L. acidophilus, L. plantarum, mikrobiyota, senkronizasyon

Destekleyen Kurum

KOSGEB

Proje Numarası

Laboratuvar Hayvanlarına (Rat ve Fare) Yönelik Araştırma Hazırlık ve Senkronizasyon Yemlerinin Geliştirilmesi

Teşekkür

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The Effect of Probiotic Feed Use on Intestinal Microbiota in Experimental Animals

Öz

Laboratory animals are frequently used in scientific studies and are of great importance. To clearly demonstrate the effectiveness of the researched in in-vivo studies, many conditions such as the breed, species and age of the animals used in the experiment, as well as environmental conditions, should be uniform in the control and test groups. However, in these experiments it is often overlooked that the intestinal microbiota, which plays an important role in the function and integrity of the gastrointestinal tract, protection of immune homeostasis and host energy metabolism, may differ in the experimental animals used, and this may adversely affect the results of the study. In this study, it was aimed to produce probiotic-containing feeds which have the potential to synchronize intestinal microbiota of laboratory animals (rat and mouse) to be used in the adaptation period before scientific studies and to investigate the effectiveness of feeds on the intestinal microbiota. According to the analysis, at the end of the storage period of the produced liquid feeds, the total number of general aerobic organisms and the number of lactic acid bacteria (L. acidophilus and L. plantarum) reached to 109 cfu/ml, however, coliform bacteria, yeast-mold and other pathogenic microorganisms were not detected. As a result of microbiota analysis, it was revealed that 10-day probiotic feed application was effective on intestinal microbiota composition in both mice and rats compared to control groups and 0. day test groups. In the literature reviews carried out to date, it has been determined that there is no study in this scope in Turkey. In this context, it was concluded that feeding experimental animals with probiotic-containing feeds that produced within the scope of this study before in-vivo studies will contribute to the improvement of the intestinal microbiota of the laboratory animals.

Anahtar Kelimeler

Laboratory animals, L. acidophilus, L. plantarum, microbiota, synchronization

Proje Numarası

Laboratuvar Hayvanlarına (Rat ve Fare) Yönelik Araştırma Hazırlık ve Senkronizasyon Yemlerinin Geliştirilmesi

Kaynakça

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