Nohutta Ascochyta Yanıklıklık Etmeni Ascochyta Rabıeı'nın TÜRK İzolatlarının Toksin Üretimi ve DNA Sekans Analizleri

Yıl 2010, Cilt: 39 Sayı: 1-2-3, 31 - 38, 01.06.2010

F. Sara Dolar

Öz

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Anahtar Kelimeler

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Toxin Production and DNA Sequence Analysis of Turkish Isolates of Ascochyta rabiei, the Causual Agent of Ascochyta Blight in Chickpea

Öz

In this study, twenty isolates of Ascochyta rabiei were isolated from disease-enfecteol chickpea plants which were collected from chickpea growing areas in Turkey. In order to determine solanapyrone production of these isolates, the fungus was grown on Czapek Dox liquid culture medium (CDLCM) for 12 day at two different temperatures. Quantitation of solanapyrones was determined with HPLC analyses. The results demonstrated that all isolates produced solanapyrone A in CDLCM at 200C but not at 300C. Confirmation of the identity of the pathogen was sought by sequence analysis of rDNA. These experiments showed that the sequences of the internal transcribed spacers (ITS) and 5.8 S gene of the seven isolates, which were identical to each other, were also identical to that of a Pakistan isolate of A.rabiei. rDNA sequences of the PCR products of isolates of A.rabiei which were produced different amount of toxin were same

Anahtar Kelimeler

Solanapyrone, Ascochyta rabiei, Chickpea, DNA Sequence