Investigation of The Effects of The Different Extraction Conditions on Phenolic Compounds Extraction from Onion Skin

Öz

Onion skin, which is a waste from the onion cultivation and processing industry, is one of the richest natural sources of phenolic compounds. In this study, the onion skin was extracted using different solvents and extraction times at various solid:solvent ratios to optimize the total phenolic compounds (TPC) and total flavonoid compounds (TFC) of the onion skin extract. The greatest content of TPC (61.29 mg GAE/g) and TFC (56.83 mg QE/g) of the extracts were obtained with 50% ethanol (ethanol/water; v/v) in 0.2 g/10 mL solid:solvent ratio. The highest TPC value was detected at the end of 90 minutes for 0.2 g/5 mL (55.25 mg GAE/g) and at the end of 120 minutes for 0.2 g/10 mL (60.14 mg GAE/g), while the highest TFC (51.06; 56.55 mg QE/g) value for both solid:solvent ratios were detected at the end of 120 minutes. It was found that the TPC and TFC of the onion skin were strongly influenced by the extraction condition. The findings of this study show that onion skin extract is a good source of phenolic compounds and emphasize that the extraction parameters used in obtaining onion skin extract are important.

Anahtar Kelimeler

• Bioactive compound
• Extraction
• Onion skin
• Quercetin
• Waste

Soğan Kabuğundan Fenolik Bileşik Ekstraksiyonu Üzerine Farklı Ekstraksiyon Koşullarının Etkisinin Araştırılması

Öz

Soğan yetiştirme ve işleme endüstrisinin atıklarından biri olan soğan kabuğu, fenolik bileşiklerin en zengin doğal kaynaklarından biridir. Bu çalışmada, soğan kabuğu ekstraktındaki toplam fenolik madde (TFM) ve toplam flavonoid (TFL) değerlerini optimize etmek için soğan kabuğu farklı katı:çözücü oranlarında çeşitli çözücüler ve ekstraksiyon süreleri kullanılarak ekstrakte edilmiştir. En yüksek TFM (61.29 mg GAE/g) ve TFL (56.83 mg KE/g) içeriği 0.2 g/10 mL katı:çözücü oranında %50 etanol (etanol/su; h/h) kullanılarak elde edilen ekstraktta tespit edilmiştir. En yüksek TFM değeri 0.2 g/5 mL için (55.25 mg GAE/g) 90 dakika sonunda ve 0.2 g/10 mL için (60.14 mg GAE/g) 120 dakika sonunda belirlenirken, her iki katı:sıvı oranı için en yüksek TFL (51.06; 56.55 mg KE/g) değeri 120 dakika sonunda saptanmıştır. Soğan kabuğundan elde edilen ekstraktın TFM ve TFL değerlerinin ekstraksiyon koşullarından önemli düzeyde etkilendiği tespit edilmiştir. Bu çalışmanın bulguları, soğan kabuğu ekstraktının iyi bir fenolik bileşik kaynağı olduğunu göstermekte ve bu ekstraktın eldesinde kullanılan ekstraksiyon parametrelerinin önemli olduğunu vurgulamaktadır.

Anahtar Kelimeler

• Biyoaktif bileşik
• Ekstraksiyon
• Soğan kabuğu
• Kuersetin
• Atık

Kaynakça

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