Preparation of Amperometric Enzyme Electrode Based on Polyphenol Oxidase for Determination of Phenolic Compounds

Yıl 2010, Cilt: 30 Sayı: 3, 955 - 978, 01.09.2010

Derya Koyuncu Zeybek Bülent Zeybek Nuran Özçiçek Pekmez Esma Kılıç Şule Pekyardımcı

Öz

In this study, amperometric enzyme electrodes based on polymer film for determination phenolic compounds were prepared. For this purpose platin electrodes were modified by electropolymerization using asetonitril-water mixture containing o-phenylenediamine (o-PD) or o-phenylenediamine C and -0.20 V (vs. Ag/AgCl) respectively. The linear working range of 1.96×10-6-3.5×10-5 M. Response time, reusability, life time, and amperometric responses to other phenolic compounds were also investigated.and 3-methylthiophene (3MT). Polyphenol oxidase was immobilized onto Pt/PoPD and Pt/PoPD-3MT electrodes and amperomeric responses to pyrocatechol were investigated. Pt/PoPD-3MT/POx electrode that obtained higher sensitivity was used for determination of working conditions and performance factors. Optimum type of buffer, buffer concentration, pH, temperature and working potential were determinated as phos

Anahtar Kelimeler

Amperometry, Enzyme electrode, Phenolic compounds, Polyphenol oxidase, poly o phenylenediamine, 3 Methylthiophene.

Fenolik Bileşiklerin Tayini için Polifenol Oksidaz Temelli Amperometrik Enzim Elektrot Hazırlanması

Öz

Bu çalışmada, fenolik bileşiklerin tayini için polimer film temelli amperometrik enzim elektrotlar hazırlandı. Bu amaçla, o-fenilendiamin (o-PD) veya o-PD ve 3-metiltiyofen (3-MT) içeren asetonitril-su karışımı kullanılarak platin elektrotlar elektropolimerizasyon yoluyla modifiye edildi. Hazırlanan Pt/PoPD ve Pt/PoPD-3MT elektrotlara polifenol oksidaz (POx) enzimi immobilize edildi ve elektrotların pirokateşole duyarlıkları belirlendi. Daha yüksek duyarlığın elde edildiği Pt/PoPD-3MT/POx elektrot ile çalışma koşulları ve performans faktörleri çalışıldı. Pt/PoPD-3MT/POx elektrot için optimum tampon cinsi, tampon derişimi, pH\'sı, sıcaklık ve çalışma potansiyeli sırasıyla fosfat; 0,10 M; 7,0; 25°C ve Ag/AgCl\'e karşı -0,20 V olarak belirlendi. Enzim elektrodun doğrusal çalışma aralığı 1,96×10–6- 3,50×10-5M olarak belirlendi. Enzim

Anahtar Kelimeler

Amperometri, Enzim elektrot, Fenolik bileşikler, Polifenol oksidaz, Poli ofenilendiamin, 3 Metiltiyofen.

Kaynakça

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