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Determination of Reference Interval of Serum Trimethylamine-N-Oxide In Humans By LC-MS/MS Method: Retrospective Study

Cilt: 36 Sayı: 2026 5 Mart 2026
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Determination of Reference Interval of Serum Trimethylamine-N-Oxide In Humans By LC-MS/MS Method: Retrospective Study

Öz

Aim: The aim of our study is to determine the reference range of serum TMAO levels in individuals aged 18-65 using the LC-MS/MS method.

Methods: TMAO levels in serum samples from 185 healthy individuals aged 18-65 were determined using the LC-MS/MS method. Statistical analysis classified the data by gender and age. The lower and upper limits with a 90% confidence interval were estimated using the robust method. Outliers were identified and excluded from the log-transformed data using the Tukey criterion. Skewness and kurtosis estimates were based on log-transformed data.

Results: TMAO levels were similar between men and women, with median values of 180 ng/mL and 200 ng/mL, respectively (Z = –0.476, p=0.633). Additionally, no significant difference was observed in TMAO levels between women under 30 years of age and those aged 30 and above (median: 178 ng/mL vs. 186 ng/mL; Z = –0.739, p = 0.460). However, men aged 30 and above had significantly higher TMAO levels compared to men under 30 (median: 215 ng/mL vs. 191 ng/mL; Z = –2.225, p = 0.026). The reference interval for serum TMAO was determined as follows: 27–974 ng/mL in men under 30 years, 52–942 ng/mL in men aged 30 and above, 45–655 ng/mL in women under 30 years, and 33–539 ng/mL in women aged 30 and above.

Conclusions: This study determined age- and gender-specific reference intervals for serum TMAO levels using the LC-MS/MS method. To our knowledge, this is the first study to establish such reference intervals using this analytical technique. The findings are expected to provide valuable data for future clinical and epidemiological studies involving TMAO as a biomarker.

Anahtar Kelimeler

TMAO, LCMS/MS, Reference Interval

Destekleyen Kurum

There was no source of funding for the study.

Etik Beyan

Ethics committee approval numbered 2023/160 was received for the study from Selcuk University Local Ethics Committee.

Kaynakça

  1. 1. Gatarek P, Kaluzna-Czaplinska J. Trimethylamine N-oxide (TMAO) in human health. EXCLI J. 2021;20:301.
  2. 2. Dalla Via A, Gargari G, Taverniti V, Rondini G, Velardi I, Gambaro V, et al. Urinary TMAO levels are associated with the taxonomic composition of the gut microbiota and with the choline TMA-lyase gene (cutC) harbored by Enterobacteriaceae. Nutrients. 2019;12(1):62.
  3. 3. He W, Luo Y, Liu JP, Sun N, Guo D, Cui LL, et al. Trimethylamine N-oxide, a gut microbiota-dependent metabolite, is associated with frailty in older adults with cardiovascular disease. Clin Interv Aging. 2020;15:1809–20.
  4. 4. Schneider C, Okun JG, Schwarz KV, Hauke J, Zorn M, Nürnberg C, et al. Trimethylamine‐N‐oxide is elevated in the acute phase after ischaemic stroke and decreases within the first days. Eur J Neurol. 2020;27(8):1596–603.
  5. 5. Tang WW, Wang Z, Fan Y, Levison B, Hazen JE, Donahue LM, et al. Prognostic value of elevated levels of intestinal microbe-generated metabolite trimethylamine-N-oxide in patients with heart failure: refining the gut hypothesis. J Am Coll Cardiol. 2014;64(18):1908–14.
  6. 6. Trøseid M, Ueland T, Hov JR, Svardal A, Gregersen I, Dahl CP, et al. Microbiota‐dependent metabolite trimethylamine‐N‐oxide is associated with disease severity and survival of patients with chronic heart failure. J Intern Med. 2015;277(6):717–26.
  7. 7. Suzuki T, Heaney LM, Jones DJ, Ng LL. Trimethylamine N-oxide and risk stratification after acute myocardial infarction. Clin Chem. 2017;63(1):420–8.
  8. 8. Bain MA, Faull R, Fornasini G, Milne RW, Evans AM. Accumulation of trimethylamine and trimethylamine-N-oxide in end-stage renal disease patients undergoing hemodialysis. Nephrol Dial Transplant. 2006;21(5):1300–4.
  9. 9. Tai N, Wong FS, Wen L. The role of gut microbiota in the development of type 1, type 2 diabetes mellitus and obesity. Rev Endocr Metab Disord. 2015;16:55–65.
  10. 10. Erdman SE, Poutahidis T. Gut bacteria and cancer. Biochim Biophys Acta Rev Cancer. 2015;1856(1):86–90.

Kaynak Göster

APA
Ovalı, F., Vatansev, H., & Akyürek, F. (2026). Determination of Reference Interval of Serum Trimethylamine-N-Oxide In Humans By LC-MS/MS Method: Retrospective Study. Genel Tıp Dergisi, 36(2026), 1-5. https://doi.org/10.54005/geneltip.1549005
AMA
1.Ovalı F, Vatansev H, Akyürek F. Determination of Reference Interval of Serum Trimethylamine-N-Oxide In Humans By LC-MS/MS Method: Retrospective Study. Genel Tıp Derg. 2026;36(2026):1-5. doi:10.54005/geneltip.1549005
Chicago
Ovalı, Fadime, Hüsamettin Vatansev, ve Fikret Akyürek. 2026. “Determination of Reference Interval of Serum Trimethylamine-N-Oxide In Humans By LC-MS/MS Method: Retrospective Study”. Genel Tıp Dergisi 36 (2026): 1-5. https://doi.org/10.54005/geneltip.1549005.
EndNote
Ovalı F, Vatansev H, Akyürek F (01 Mart 2026) Determination of Reference Interval of Serum Trimethylamine-N-Oxide In Humans By LC-MS/MS Method: Retrospective Study. Genel Tıp Dergisi 36 2026 1–5.
IEEE
[1]F. Ovalı, H. Vatansev, ve F. Akyürek, “Determination of Reference Interval of Serum Trimethylamine-N-Oxide In Humans By LC-MS/MS Method: Retrospective Study”, Genel Tıp Derg, c. 36, sy 2026, ss. 1–5, Mar. 2026, doi: 10.54005/geneltip.1549005.
ISNAD
Ovalı, Fadime - Vatansev, Hüsamettin - Akyürek, Fikret. “Determination of Reference Interval of Serum Trimethylamine-N-Oxide In Humans By LC-MS/MS Method: Retrospective Study”. Genel Tıp Dergisi 36/2026 (01 Mart 2026): 1-5. https://doi.org/10.54005/geneltip.1549005.
JAMA
1.Ovalı F, Vatansev H, Akyürek F. Determination of Reference Interval of Serum Trimethylamine-N-Oxide In Humans By LC-MS/MS Method: Retrospective Study. Genel Tıp Derg. 2026;36:1–5.
MLA
Ovalı, Fadime, vd. “Determination of Reference Interval of Serum Trimethylamine-N-Oxide In Humans By LC-MS/MS Method: Retrospective Study”. Genel Tıp Dergisi, c. 36, sy 2026, Mart 2026, ss. 1-5, doi:10.54005/geneltip.1549005.
Vancouver
1.Fadime Ovalı, Hüsamettin Vatansev, Fikret Akyürek. Determination of Reference Interval of Serum Trimethylamine-N-Oxide In Humans By LC-MS/MS Method: Retrospective Study. Genel Tıp Derg. 01 Mart 2026;36(2026):1-5. doi:10.54005/geneltip.1549005