The Anticancer Effect of Curcumin and Piperine Combination in Non-Small Cell Lung Cancer Cells
Öz
Aim: Curcumin is known to have anticancer effects in different types of cancer, but its bioavailability is low due to its poor absorption and rapid metabolism. Piperine is known to increase the bioavailability of numerous therapeutic agents such as curcumin. This study aimed to investigate the possible synergistic effect of combined curcumin and piperine treatment in the A549 non-small cell lung cancer cell line.
Materials and Methods: After IC50 doses were determined using the MTT technique, the mRNA expression levels of genes involved in apoptosis and the cell cycle (p53, TRAIL-1, TRAIL-2, NF-κB, IKKB, Bax, and Bcl-2), as well as the effects of oxidative stress, DNA damage, and inflammation (TNF-α, TGF-β, IL-1β and DEF-β2) of the active substances were determined in the experimental groups.
Results: The results showed that curcumin and piperine dose-dependently reduced cell viability. DNA damage levels were the same as in the control group when curcumin and piperine were administered together. TAS level was found to be high compared to the control group, while TOS and OSI levels were low. It was also observed that inflammatory cytokines (TNF-α, TGF-β, IL-1β, and DEF-β2) decreased. Molecular analysis results also showed that the combination of curcumin and piperine up-regulated p53, Bax, TRAIL-2, NF-κB mRNA expression levels, while down-regulated TRAIL-1, IKKB, and Bcl-2 mRNA expression levels.
Conclusions: These results suggest that the combination of curcumin and piperine could be considered as an adjuvant treatment approach to current therapies in the treatment of non-small cell lung cancer.
Anahtar Kelimeler
Curcumin, Piperin, A549 cells, bioavailability, combination therapy
Destekleyen Kurum
Proje Numarası
Etik Beyan
Teşekkür
Kaynakça
- Referans1. Zou B, Zhou XL, Lai SQ, Liu JC. Notch signaling and non-small cell lung cancer. Oncol Lett. 2018;15:3415-21.
- Referans2. Diniz G, Unlu I, Komurcuoglu B. Histopathological and molecular features of lung cancer. Anatol J Gen Med Res. 2017;27:77-87.
- Referans3. Mehta HJ, Patel V, Sadikot RT. Curcumin and lung cancer--a review. Target Oncol. 2014;9:295-310.
- Referans4. Perrone D, Ardito F, Giannatempo G, et al. Biological and therapeutic activities, and anticancer properties of curcumin. Exp Ther Med. 2015;10:1615-23.
- Referans5. Noorafshan A, Ashkani-Esfahani S. A review of therapeutic effects of curcumin. Curr Pharm Des. 2013;19:2032-46.
- Referans6. Devassy JG, Nwachukwu ID, Jones PJ. Curcumin and cancer: barriers to obtaining a health claim. Nutr Rev. 2015;73:155-65.
- Referans7. Maria DN, Mishra SR, Wang L, Abd-Elgawad AEH, Soliman OAE, El-Dahan MS, Jablonski MM. Water-soluble complex of curcumin with cyclodextrins: enhanced physical properties for ocular drug delivery. Curr Drug Deliv. 2017;14(6):875-86.
- Referans8. Patel SS, Acharya A, Ray RS, Agrawal R,Raghuwanshi R, Jain P. Cellular and molecular mechanisms of curcumin in prevention and treatment of disease. Crit Rev Food Sci Nutr. 2020;60(6):887-939.
- Referans9. Haq IU, Imran M, Nadeem M, Tufail T, Gondal TA, Mubarak MS. Piperine: A review of its biological effects. Phytother Res. 2021;35(2):680-700.
- Referans10. Smilkov K, Ackova DG, Cvetkovski A, Ruskovska T, Vidovic B, Atalay M. Piperine: old spice and new ntraceutical? Curr Pharm Des. 2019;25:1729-39.