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Monitoring Illicit Drugs and New Psychoactive Substances in Urban Wastewater of Konya Using LC-MS/MS: A Prospective Pilot Study

Cilt: 36 Sayı: 2026 13 Ekim 2026
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Monitoring Illicit Drugs and New Psychoactive Substances in Urban Wastewater of Konya Using LC-MS/MS: A Prospective Pilot Study

Öz

Aim: This study aimed to identify illicit drugs and new psychoactive substances in wastewater reaching a treatment plant in Konya, quantify their concentrations using high-sensitivity analytical methods, and provide preliminary data on their occurrence and temporal distribution.
Materials and Methods: Throughout August 2025, 24-hour composite wastewater samples (n=12) were collected on predefined routine sampling days from a wastewater treatment plant in Konya. Samples were pre-concentrated using solid-phase extraction, quantitatively analyzed by LC-MS/MS, and positive findings were confirmed using high-resolution Orbitrap LC-MS. Analyses were conducted in accordance with validated institutional protocols following internal standard correction.
Results: Reliable quantitative results were obtained for 10 analytes detected in wastewater samples. The number of analytes identified per sample ranged from one to nine. Pharmaceutical compounds were detected in all or most samples, whereas indicators of illicit drugs and psychoactive substances were observed only on a limited number of sampling days. Heat-map analysis demonstrated that methamphetamine, MDMB-BUTINACA, and benzoylecgonine exhibited discontinuous temporal patterns restricted to specific days.
Conclusions: The findings of this prospective pilot study suggest that wastewater-based epidemiology may serve as a feasible approach for monitoring the occurrence and temporal distribution of illicit drugs and new psychoactive substances in urban wastewater. Monitoring strategies incorporating increased sampling frequency and normalization using flow and population data may improve the reliability and comparability of temporal assessments.

Anahtar Kelimeler

illicit drugs, mass spectrometry, new psychoactive substances, synthetic cannabinoids, Wastewater

Kaynakça

  1. 1. Daglioglu N, Atasoy Aydın A, Yavuz Güzel E, Ertas BS. Wastewater analysis of illicit substances as a means of detecting substance abuse. In: Ögel K, editor. Effective Drug Control Strategies in Northern Cyprus: Challenges and Opportunities in 2024. Vol 1. Emanate - Highlights from the Drug Abuse and Addiction Studies Sessions. Emanate Publishing House Ltd.; 2024. p.5-15.
  2. 2. Yi R, Zeng T, Chen J, Liu D, Yang X, Zhao M, et al. Wastewater-based epidemiology: assessing illicit drug usage and impact through an innovative approach. Water (Basel). 2023;15(23):4192.
  3. 3. Ort C, Bijlsma L, Castiglioni S, Covaci A, de Voogt P, Emke E, et al. Wastewater analysis for community-wide drugs use assessment. In: New psychoactive substances. Springer; 2018. p. 543–566.
  4. 4. de Campos EG, de Martinis ECP, de Martinis BS. Forensic analysis of illicit drugs and novel psychoactive substances in wastewater: a review of toxicological, chemical and microbiological aspects. Braz J Anal Chem. 2022;9(34):15–34.
  5. 5. Banta-Green CJ, Field JA, Chiaia AC, Sudakin DL, Power L, de Montigny L. The spatial epidemiology of cocaine, methamphetamine and 3,4-methylenedioxymethamphetamine (MDMA) use: a demonstration using a population measure of community drug load derived from municipal wastewater. Addiction. 2009;104(11):1874-1880.
  6. 6. Picó Y, Barceló D. Mass spectrometry in wastewater-based epidemiology for the determination of small and large molecules as biomarkers of exposure: toward a global view of environment and human health under the COVID-19 outbreak. ACS Omega. 2021;6(46):30865-30872.
  7. 7. Badawy MEI, El-Nouby MAM, Kimani PK, Lim LW, Rabea EI. A review of the modern principles and applications of solid-phase extraction techniques in chromatographic analysis. Anal Sci. 2022;38:1457-1487.
  8. 8. Xolo T, Mahlambi P. Molecularly imprinted polymers as solid-phase and dispersive solid-phase extraction sorbents in the extraction of antiretroviral drugs in water: adsorption, selectivity and reusability studies. J Anal Sci Technol. 2024;15:11.
  9. 9. Kuloglu Genc M, Mercan S, Yayla M, Tekin Bulbul T, Adioren C, Simsek SZ, et al. Monitoring geographical differences in illicit drugs, alcohol, and tobacco consumption via wastewater-based epidemiology: six major cities in Turkey. Sci Total Environ. 2021;797:149156.
  10. 10. Mercan S, Kuloglu M, Tekin T, Turkmen Z, Dogru AO, Safran AN, et al. Wastewater-based monitoring of illicit drug consumption in Istanbul: preliminary results from two districts. Sci Total Environ. 2019;656:231-238.

Kaynak Göster

APA
Depreli, A., Altınok, M., Yüce Boyraz, T., Sarı, S., Aydemir, Ö., & Doğan, K. H. (2026). Monitoring Illicit Drugs and New Psychoactive Substances in Urban Wastewater of Konya Using LC-MS/MS: A Prospective Pilot Study. Genel Tıp Dergisi, 36(2026), 1-6. https://doi.org/10.54005/geneltip.1883924
AMA
1.Depreli A, Altınok M, Yüce Boyraz T, Sarı S, Aydemir Ö, Doğan KH. Monitoring Illicit Drugs and New Psychoactive Substances in Urban Wastewater of Konya Using LC-MS/MS: A Prospective Pilot Study. Genel Tıp Derg. 2026;36(2026):1-6. doi:10.54005/geneltip.1883924
Chicago
Depreli, Ahmet, Mehmet Altınok, Tuğçe Yüce Boyraz, Salih Sarı, Önder Aydemir, ve Kamil Hakan Doğan. 2026. “Monitoring Illicit Drugs and New Psychoactive Substances in Urban Wastewater of Konya Using LC-MS/MS: A Prospective Pilot Study”. Genel Tıp Dergisi 36 (2026): 1-6. https://doi.org/10.54005/geneltip.1883924.
EndNote
Depreli A, Altınok M, Yüce Boyraz T, Sarı S, Aydemir Ö, Doğan KH (01 Ekim 2026) Monitoring Illicit Drugs and New Psychoactive Substances in Urban Wastewater of Konya Using LC-MS/MS: A Prospective Pilot Study. Genel Tıp Dergisi 36 2026 1–6.
IEEE
[1]A. Depreli, M. Altınok, T. Yüce Boyraz, S. Sarı, Ö. Aydemir, ve K. H. Doğan, “Monitoring Illicit Drugs and New Psychoactive Substances in Urban Wastewater of Konya Using LC-MS/MS: A Prospective Pilot Study”, Genel Tıp Derg, c. 36, sy 2026, ss. 1–6, Eki. 2026, doi: 10.54005/geneltip.1883924.
ISNAD
Depreli, Ahmet - Altınok, Mehmet - Yüce Boyraz, Tuğçe - Sarı, Salih - Aydemir, Önder - Doğan, Kamil Hakan. “Monitoring Illicit Drugs and New Psychoactive Substances in Urban Wastewater of Konya Using LC-MS/MS: A Prospective Pilot Study”. Genel Tıp Dergisi 36/2026 (01 Ekim 2026): 1-6. https://doi.org/10.54005/geneltip.1883924.
JAMA
1.Depreli A, Altınok M, Yüce Boyraz T, Sarı S, Aydemir Ö, Doğan KH. Monitoring Illicit Drugs and New Psychoactive Substances in Urban Wastewater of Konya Using LC-MS/MS: A Prospective Pilot Study. Genel Tıp Derg. 2026;36:1–6.
MLA
Depreli, Ahmet, vd. “Monitoring Illicit Drugs and New Psychoactive Substances in Urban Wastewater of Konya Using LC-MS/MS: A Prospective Pilot Study”. Genel Tıp Dergisi, c. 36, sy 2026, Ekim 2026, ss. 1-6, doi:10.54005/geneltip.1883924.
Vancouver
1.Ahmet Depreli, Mehmet Altınok, Tuğçe Yüce Boyraz, Salih Sarı, Önder Aydemir, Kamil Hakan Doğan. Monitoring Illicit Drugs and New Psychoactive Substances in Urban Wastewater of Konya Using LC-MS/MS: A Prospective Pilot Study. Genel Tıp Derg. 01 Ekim 2026;36(2026):1-6. doi:10.54005/geneltip.1883924