The Evaluation of The Relation Between Nasal Polyp Etiopathogenesis and the Amount of Biofilm

Yıl 2022, Cilt: 32 Sayı: 1, 36 - 38, 28.02.2022

Kazım Bozdemir , Elif Ersoy Çallıoğlu , Bülent Ulusoy , Selami Candan , Serkan Şerifler , Nurcan Özyurt Koçakoğlu , Yuce İslamoglu

https://doi.org/10.54005/geneltip.1014726

Öz

Objective: To evaluate the effect of biofilm layer on polyp formation in nasal polyposis by comparing the amount of biofilm layer on polyp tissue and the normal mucosa.
Material and Methods: This study is a prospective study. 14 patients who underwent functional endoscopic sinus surgery (FESS) for nasal polyposis were evaluated. Patients were had no history of previous FESS. Tissue samples were obtained from lower turbinate (Group 1 n:14) and from nasal polyp (Group 2, n:14) of the same nasal cavity. Biofilm presence was identified using scanning electron microscopic (SEM) morphological findings. In biofilm positive samples, the presence of biofilm in less than 25% of the surface area was classified as (+), between 25-50% as (++) , and over 50% as (+++).
Results: In Group 2, with SEM imaging, (+++) biofilm presence was detected in 9 patients and (++) biofilm was detected in 5 patients. In Group 1, no biofilm was detected 8 patients, while; (+++), (++), and (+) biofilm presence was detected in 1, 2, and 3 patients respectively. Significant difference was found between group 1 and group 2 with respect to the amount of biofilm according to SEM (p =0.000).
Conclusion: In samples, statistically significant difference was found between polyp tissue and normal mucosa in terms of the amount of biofilm. However, further studies with larger patient series are required in order to reach a definitive conclusion on the effect of biofilm on pathogenesis of polyp.

Anahtar Kelimeler

Biofilm, Nasal Polyp, Microscopy, Electron, Scanning

NAZAL POLİP ETİOPATOGENEZİ VE BİOFİLM MİKTARI ARASINDAKİ İLİŞKİNİN DEĞERLENDİRİLMESİ

Öz

Amaç: Polip dokusu ve normal mukoza üzerindeki biyofilm tabakası miktarını karşılaştırarak nazal polipoziste biyofilm tabakasının polip oluşumuna etkisini değerlendirmek amaçlanmaktadır.
Materyal ve Metod: Bu çalışma, prospektif bir çalışma olup nazal polipozis nedeniyle fonksiyonel endoskopik sinüs cerrahisi (FESS) uygulanan 14 hasta değerlendirildi. Hastalar, daha önce endoskopik sinüs cerrahisi geçirmemiş hastalardan oluşmaktaydı. Doku örnekleri, aynı nazal kaviteden alt konkadan (Grup 1, n:14) ve nazal polip dokusundan (Grup 2, n:14) alındı. Biyofilm varlığı, Scanning elektron mikroskobu (SEM) morfolojik bulgular kullanılarak tanımlandı. Biyofilm pozitif örneklerde, görüntülenen yüzey alanının %25’inden az sahada biyofilm varlığı (+), %25-50 arası (++) ve %50’den fazla sahada biyofilm varlığı (+++) olarak sınıflandırıldı.
Bulgular: Grup 2’de SEM görüntülemede 9 hastada (+++) biyofilm mevcudiyeti saptanırken 5 hastada (++) biyofilm tespit edildi. Grup 1'de ise 8 hastada biyofilm saptanmazken 1 hastada (+++), 2 hastada (++) ve 3 hastada (+) biyofilm varlığı saptandı. SEM bulgularına göre biyofilm miktarı açısından grup 1 ve grup 2 arasında anlamlı fark bulundu (p=0.000).
Sonuç: Örneklerde, polip dokusu ile normal mukoza arasında biyofilm miktarı açısından istatistiksel olarak anlamlı fark bulundu. Bununla birlikte, biyofilmin polip patogenezine etkisi konusunda kesin bir sonuca varmak için daha geniş hasta serileri ile yapılacak çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

Biyofilm, Nazal Polip, Scanning-Electron Mikroskopi

Kaynakça

• 1. Leone S, Molinaro A, Alfieri F, et al. The biofilm matrix of Pseudomonas sp. OX1 grown on phenol is mainly constituted by alginate oligosaccharides. Carbohydr Res 2006;341: 2456 –61.
• 2. O’Toole G, Kaplan HB. Biofilm formation as microbial development. Annual Reviews in Microbiology, 2000; 49-79.
• 3. Zernotti ME, Angel Villegas N, Roques Revol M, Baena-Cagnani CE, Arce Miranda JE, Paredes ME. Evidence of bacterial biofilms in nasal polyposis. J Investig Allergol Clin Immunol 2010; 20: 380–5.
• 4. Mladina R, Poje G, Vukovi K, Risti M, Musi S. Biofilm in nasal polyps. Rhinology 2008; 46:302–7.
• 5. Korkmaz H, Ocal B, Tatar EC, et al. Biofilms in chronicrhinosinusitis with polyps: is eradicationpossible? Eur Arch Otorhinolaryngol 2014; 271:2695-702.
• 6. Tatar EÇ, Tatar I, Ocal B, et al. Prevalence of biofilms and their response to medical treatment in chronic rhinosinusitis without polyps. Otolaryngol Head Neck Surg 2012; 146:669-75.
• 7. Saylam G, Tatar EC, Tatar I, Ozdek A, Korkmaz H (2010). Association of adenoid surface biofilm formation and chronic otitis media with effusion. Arch Otolaryngol Head Neck Surg 2010; 136:550-5.
• 8. Vuong C, Kocianova S, Voyich JM, et al. (2010) A crucial role for exopolysaccharide modification in bacterial biofilm formation, immune evasion, and virulence. J Biol Chem 2010; 279:54881-6.
• 9. Tripathi A, Kern R, Conley DB, et al. Staphylococcal exotoxins and nasal polyposis: analysis of systemic and local responses. Am J Rhinol 2005; 19:327-33.
• 10. Seiberling KA, Conley DB, Tripathi A, et al. Superantigens and chronic rhinosinusitis: detection of staphylococcal exotoxin in nasal polyps. Laryngoscope 2005; 115: 1580-5.
• 11. Bernstein JM, Ballow M, Schliever PM, Rich G, Allen C, Dryja D. A superantigen hypothesis for the pathogenesis of chronic hyperplasic sinusitis with massive nasal polyposis. Am J Rhinol 2003;17: 321-6.
• 12. Sanclement JA, Webster P, Thomas J, Ramadan HH. Bacterial biofilms in surgical specimens of patients with chronic rhinosinusitis. Laryngoscope 2005; 115: 578-82.
• 13. Galli J, Calò L, Ardito F, et al. Damage to ciliated epithelium in chronic rhinosinusitis: what is the role of bacterial biofilms? Ann Otol Rhinol Laryngol 2008; 117:902-8.
• 14. Singh P, Mehta R, Agarwal S, Mishra P. Bacterial biofilm on the sinus mucosa of healthy subjects and patients with chronic rhinosinusitis (with or without nasal polyposis). J Laryngol Otol 2015; 129: 46–9.
• 15. Bendouah Z, Barbeau J, Hamad WA, Desrosiers M. Biofilm formation by Staphylococcus aureus and Pseudomonas aeruginosa is associated with an unfavorable evolution after surgery for chronic sinusitis and nasal polyposis. Otolaryngol Head Neck Surg 2006; 134:991-996.
• 16. Sanderson AR, Leid JG, Hunsaker D. Bacterial biofilms on thesinus mucosa of human subjects with chronic rhinosinusitis. Laryngoscope 2006; 116:1121-6.
• 17. Bezerra TF, Pádua FG, Ogawa AI, Gebrim EM, Saldiva PH, Voegels RL. Biofilm in chronic sinusitis with nasal polyps: pilot study. Braz J Otorhinolaryngol 2009; 75:788-93.