Does Serum R-Spondin-1 Play a Role in PCOS Pathophysiology?

Yıl 2022, Cilt: 32 Sayı: 5, 490 - 493, 29.10.2022

Osman Başpınar Yasin Şimşek Derya Koçer Oğuzhan Sıtkı Dizdar Hatice Kayış Topaloğlu

https://doi.org/10.54005/geneltip.1111079

Öz

Objective: Many new inflammatory and metabolic markers are considered to be involved in etiology of Polycystic ovary syndrome (PCOS). R-spondin-1 (Rspo1) is a member of the roof plate-specific spondin protein family. Rspo1 levels have been associated with obesity and insulin resistance. We aimed to investigate whether Rspo1 has a role in the pathophysiology of PCOS.

Materials and Methods: This cross-sectional study was planned with newly diagnosed and untreated PCOS cases and a control group without PCOS. Patients with PCOS and healty participants were compared for Rspo1 levels. In addition, the PCOS group was also grouped according to body mass index (BMI) and Ferriman Gallwey scores (FGS) and compared in terms of Rsop1 levels.

Results: A total of 47 patients in the PCOS group and 36 participants in the control group, as total 83 participants were included in the study. PCOS and control groups had similar age and BMI. As compared to the control group, PCOS patients had significantly higher FGS, homeostasis model assessment of insulin resistance scores, Rspo1, and total testosterone levels (p<0.001, p=0.01, p=0.02, p=0.001 respectively). When subclassing PCOS patients according to BMI and FGS values, there was no statistically significant difference between Rspo1 levels in terms of both BMI and FGS values. Moreover, Rspo1 showed a significant positive correlation with total testosterone and dehydroepiandrosterone sulfate levels (p=0.03, r=0.23; p=0.08, r=0.30, respectively).

Conclusion: Rspo-1 may be associated with PCOS pathophysiology via total testosterone and dehydroepiandrosterone sulphate. Further molecular and genetic studies are needed to support this hypothesis.

Anahtar Kelimeler

R-spondin-1, Polycystic ovary syndrome, Ferriman Gallwey scores

Destekleyen Kurum

none

Proje Numarası

none

Teşekkür

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PCOS Patofizyolojisinde Serum R-Spondin-1'in Bir Rolü Var mıdır?

Öz

Amaç: Pek çok yeni inflamatuar ve metabolik belirtecin Polikistik Over Sendromu (PCOS) etiyolojisinde rol oynadığı düşünülmektedir. R-spondin-1 (Rspo1), tepe tabakaya özgü spondin protein ailesinin bir üyesidir. Rspo1 seviyeleri obezite ve insülin direnci ile ilişkilendirilmiştir. Rspo1'in PCOS patofizyolojisinde rolü olup olmadığını araştırmayı amaçladık.

Gereç ve Yöntem: Bu kesitsel çalışma, yeni tanı konmuş ve tedavi edilmemiş PCOS olguları ve PCOS'u olmayan bir kontrol grubu ile planlandı. PCOS'lu hastalar ve sağlıklı katılımcılar, Rspo1 seviyeleri için karşılaştırıldı. Ayrıca PCOS grubu vücut kitle indeksi (VKİ) ve Ferriman Gallwey skorlarına (FGS) göre de gruplandırılarak Rsop1 düzeyleri açısından karşılaştırıldı.

Bulgular: PCOS grubunda 47, kontrol grubunda 36 olmak üzere toplam 83 katılımcı çalışmaya dahil edildi. PCOS ve kontrol grupları benzer yaş ve VKİ'ye sahipti. Kontrol grubu ile karşılaştırıldığında, PCOS hastalarının FGS, insülin direnci skorlarının homeostaz modeli değerlendirmesi, Rspo1 ve toplam testosteron düzeyleri anlamlı olarak daha yüksekti (sırasıyla p<0,001, p=0.01, p=0.02, p=0.001). PCOS hastaları BMI ve FGS değerlerine göre alt sınıflara ayrıldığında, hem BMI hem de FGS değerleri açısından Rspo1 seviyeleri arasında istatistiksel olarak anlamlı bir fark yoktu. Ayrıca, Rspo1, toplam testosteron ve dehidroepiandrosteron sülfat seviyeleri ile anlamlı bir pozitif korelasyon gösterdi (sırasıyla p=0.03, r=0.23; p=0.08, r=0.30).

Sonuç: Rspo-1, toplam testosteron ve dehidroepiandrosteron sülfat yoluyla PCOS patofizyolojisi ile ilişkilendirilebilir. Bu hipotezi desteklemek için daha fazla moleküler ve genetik çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

R-spondin-1, Polikistik over sendromu, Ferriman Gallwey skorları

Proje Numarası

none

Kaynakça

• Fauser BC, Tarlatzis BC, Rebar RW, Legro RS, Balen AH, Lobo R, et al. Consensus on women's health aspects of polycystic ovary syndrome (PCOS): the Amsterdam ESHRE/ASRM-Sponsored 3rd PCOS Consensus Workshop Group. Fertil Steril. 2012;97(1):28-38. e25.
• Tsilchorozidou T, Overton C, Conway GS. The pathophysiology of polycystic ovary syndrome. Clin Endocrinol (Oxf). 2004;60(1):1-17.
• Goodarzi MO, Dumesic DA, Chazenbalk G, Azziz R. Polycystic ovary syndrome: etiology, pathogenesis and diagnosis. Nat Rev Endocrinol. 2011;7(4):219-31.
• Escobar-Morreale HF, Luque-Ramirez M, San Millan JL. The molecular-genetic basis of functional hyperandrogenism and the polycystic ovary syndrome. Endocr Rev. 2005;26(2):251-82.
• Kelly CC, Lyall H, Petrie JR, Gould GW, Connell JM, Sattar N. Low grade chronic inflammation in women with polycystic ovarian syndrome. J Clin Endocrinol Metab. 2001;86(6):2453-5.
• Escobar-Morreale HF, Calvo RM, Villuendas G, Sancho J, San Millan JL. Association of polymorphisms in the interleukin 6 receptor complex with obesity and hyperandrogenism. Obes Res. 2003;11(8):987-96.
• Frishman WH. Biologic markers as predictors of cardiovascular disease. Am J Med. 1998;104(6A):18S-27S.
• Fernandez-Real JM, Ricart W. Insulin resistance and chronic cardiovascular inflammatory syndrome. Endocr Rev. 2003;24(3):278-301.
• Dandona P, Aljada A, Chaudhuri A, Mohanty P, Garg R. Metabolic syndrome: a comprehensive perspective based on interactions between obesity, diabetes, and inflammation. Circulation. 2005;111(11):1448-54.
• Usta A, Avci E, Bulbul CB, Kadi H, Adali E. The monocyte counts to HDL cholesterol ratio in obese and lean patients with polycystic ovary syndrome. Reprod Biol Endocrinol. 2018;16(1):34.
• Kim KA, Wagle M, Tran K, Zhan X, Dixon MA, Liu S, et al.R-Spondin family members regulate the Wnt pathway by a common mechanism. Mol Biol Cell. 2008;19(6):2588-96.
• Welters HJ, Kulkarni RN. Wnt signaling: relevance to beta-cell biology and diabetes. Trends Endocrinol Metab. 2008;19(10):349-55.
• Kang YE, Kim JM, Yi HS, Joung KH, Lee JH, Kim HJ, et al.Serum R-Spondin 1 Is a New Surrogate Marker for Obesity and Insulin Resistance. Diabetes Metab J. 2019;43(3):368-76.
• Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome (PCOS). Hum Reprod. 2004;19(1):41-7.
• Obesity: preventing and managing the global epidemic. Report of a WHO consultation. World Health Organ Tech Rep Ser. 2000;894:i-xii, 1-253.
• Hatch R, Rosenfield RL, Kim MH, Tredway D. Hirsutism: implications, etiology, and management. Am J Obstet Gynecol. 1981;140(7):815-30.
• Qu M, Xiong L, Lyu Y, Zhang X, Shen J, Guan J, et al.Establishment of intestinal organoid cultures modeling injury-associated epithelial regeneration. Cell Res. 2021;31(3):259-71.
• Moran LJ, Misso ML, Wild RA, Norman RJ. Impaired glucose tolerance, type 2 diabetes and metabolic syndrome in polycystic ovary syndrome: a systematic review and meta-analysis. Hum Reprod Update. 2010;16(4):347-63.
• Kim KA, Kakitani M, Zhao J, Oshima T, Tang T, Binnerts M, et al.Mitogenic influence of human R-spondin1 on the intestinal epithelium. Science. 2005;309(5738):1256-9.
• Kim KA, Zhao J, Andarmani S, Kakitani M, Oshima T, Binnerts ME, et al.R-Spondin proteins: a novel link to beta-catenin activation. Cell Cycle. 2006;5(1):23-6.
• Nam JS, Turcotte TJ, Yoon JK. Dynamic expression of R-spondin family genes in mouse development. Gene Expr Patterns. 2007;7(3):306-12.
• Wong VSC. Role of R-Spondin-1 in the Regulation of Pancreatic β-Cell Behaviour. University of Toronto. 2011.
• 9. Pharmacologic Approaches to Glycemic Treatment: Standards of Medical Care in Diabetes-2020. Diabetes Care. 2020;43(Suppl 1):S98-S110.
• Chassot AA, Ranc F, Gregoire EP, Roepers-Gajadien HL, Taketo MM, Camerino G, et al.Activation of beta-catenin signaling by Rspo1 controls differentiation of the mammalian ovary. Hum Mol Genet. 2008;17(9):1264-77.
• Parma P, Radi O, Vidal V, Chaboissier MC, Dellambra E, Valentini S, et al.R-spondin1 is essential in sex determination, skin differentiation and malignancy. Nat Genet. 2006;38(11):1304-9.