Epigallocatechin Gallate (EGCG) ve Yeşil Çay (Camellia Sinensis) Yağının Çeşitli Patojenler Üzerinde İn Vitro Antimikrobiyal Etkinliğinin Değerlendirilmesi

Yıl 2024, Cilt: 34 Sayı: 1, 38 - 42, 29.02.2024

Hale Köksoy , Cennet Ragbetli

https://doi.org/10.54005/geneltip.1333115

Öz

Amaç: Bu çalışma, Camellia sinensis çayındaki kateşinlerin polifenolik bileşeni olan Epigallokateşin gallat (EGCG) ile Camellia sinensis ekstraktının geniş bir antimikrobiyal aktiviteye sahip olduğunu incelemektedir.
Metod: Çalışma, Amerikan Tip Kültür Koleksiyonu'ndan (ATCC) seçilen bazı gram-negatif, gram-pozitif ve mantar izolatlarına karşı EGCG ve Camellia sinensis ekstraktının invitro antibakteriyel ve antifungal aktivitesini test etmiştir. Her test mikroorganizması için Epigallokateşin gallat (EGCG) ve Camellia sinensis ekstraktının minimum inhibisyon konsantrasyonu (MIC) belirlenmiştir. Genel olarak, EGCG ve Camellia sinensis ekstraktı sonuçları uyumlu bulunmuştur. Epigallokateşin gallat ve Camellia sinensis ekstraktı, tüm test organizmalarına karşı antibakteriyel ve antifungal aktivite sergilemiştir. MIC değerleri, Klinik ve Laboratuvar Standartları Enstitüsü (CLSI) tarafından önerilen çanak mikrodilüsyon yöntemleriyle, 37°C'de 16-20 saat inkübasyon sonrasında görsel olarak belirlenmiştir. Sonuç: Gram-pozitif bakteriler için Camellia sinensis ekstraktı ve EGCG MIC değerleri, gram-negatif bakterilere göre daha düşük bulunmuştur ve C. albicans için Camellia sinensis ekstraktı MIC değeri, EGCG MIC değerinden daha yüksek bulunmuştur.
Tartışma: Ancak, EGCG, C. albicans izolatı üzerinde Camellia sinensis ekstraktından daha etkili bulunmuştur. Camellia sinensis ekstraktı ve Epigallokateşin gallat, antibakteriyel ve antifungal etkileri için ileri çalışmalar için umut vadeden kaynaklar olabilir.

Anahtar Kelimeler

Epigallocatechin gallate (EGCG), Yeşil çay, Camellia sinensis, Antibakteriyal etki, Antifungal etki.

Destekleyen Kurum

No funding was received by the author.

Teşekkür

Bu çalışmada destek ve emeklerini esirgemeyen KMÜ Tıp Fakültesi Tıbbi Farmakoloji AD. Başkanı Prof. Dr. Harun Alp hocama teşekkür ederiz.

Evaluation of In Vitro Antimicrobial Activity of Epigallocatechin Gallate (EGCG) and Green Tea (Camellia Sinensis) Oil on Various Pathogens

Öz

Aim: EGCG, the polyphenolic component of Camellia sinesis catechins, and Camellia sinesis extract have broad antimicrobial activity. To investigate the invitro antibacterial and antifungal activity of the Epigallocatechin gallate and Camellia sinesis extract.
Method: The present study tested the antibacterial and antifungal activity of Epigallocatechin gallate and Camellia sinesis extract against some gram-negative, gram-positive, and fungal isolates of American Type Culture Collection (ATCC). The minimum inhibitory concentration (MIC) of Epigallocatechin gallate (EGCG) and Camellia sinensis extract were determined for each test microorganism. In general, EGCG and Camellia sinensis extract results were found to be compatible. Epigallocatechin gallate and Camellia sinesis extract exhibited antibacterial and antifungal activity against all test organisms. MIC was determined visually after 16-20 hours of incubation at 37°C according to broth microdilution methods recommended by the Clinical and Laboratory Standards Institute (CLSI).
Result: The Camellia sinesis extract and EGCG MIC for gram-positive bacteria was found to be lower than the MIC for gram-negative bacteria and the Camellia sinesis extract MIC for C. albicans was found to be higher than the EGCG MIC.
Conclusions: However, EGCG was found to be more effective on C. albicans isolate than Camellia sinensis extract. Camellia sinesis extract Epigallocatechin gallate and Camellia sinesis extract may be a promising sources of antibacterial and antifungal for further studies.

Anahtar Kelimeler

Epigallocatechin gallate (EGCG), Green tea, Camellia sinensis, Antibacterial effect, Antifungal effect.

Kaynakça

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