Investigation of Insulin-Like Growth Factor 2 mRNA Binding Protein 2 Gene Polymorphisms in Type 2 Diabetes Patients

Yıl 2024, Cilt: 34 Sayı: 3, 332 - 336, 30.06.2024

Duygu Yolal Ertural Ümit Çınkır Nurcan Aras

https://doi.org/10.54005/geneltip.1418122

Öz

Background/Aims: Type 2 diabetes (T2D) whose prevalance differs in different populations is a multifactorial disease. T2D is describes a group of clinical syndromes resulting from glucose metabolism disorders triggered by genetic or environmental factors. Insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) gene participates in insulin signaling pathway and is involved in insulin secretion. SNPs in one of these genes, IGF2BP2 gene (rs1470579 and rs4402960), have been reported to partially increase the risk of type II diabetes. The aim of this study was to investigate in a Turkish population identified associations of IGF2BP2 variants rs4402960 and rs1470579 with T2D.
Methods: We genotyped two SNPs of IGF2BP2 gene, rs1470579 and rs4402960 in 100 healthy individuals and 100 patients. DNA isolation was performed on peripheral blood samples from patients and healthy groups. The molecular analysis of rs1470579 and rs4402960 polymorphisms of IGF2BP2 gene of each individual was performed by using Real-Time PCR (Applied Biosystems) method. Relationships of genotypes and alleles frequency of IGF2BP2 polymorphisms and T2D were examined by "Chi-square" or "Likelihood ratio" tests.
Results: As a result of the genotype and allele distributions; there was association between type II diabetes patients and control group for IGF2BP2 rs1470579 (A/C) gene polymorphism (p=0.0123). The frequency of AC genotype in patients is more than the control group. However, there was no statistically significant difference genotype distribution between the type 2 diabetes patients and control group for IGF2BP2 rs4402960 (G/T) gene polymorphisms. There was no association between the patients and the control group for TT and GG+GT genotype distribution (p=0.8847).
Conclusions: The results showed that the IGF2BP2 gene rs1470579 and rs4402960 polymorphisms were associated with T2D in a Turkish population (OR = 2.002, 95% CI 1.170–3.426, p < 0.05; OR = 1.879, 95% CI 1.110–3.182, p< 0.05). This is the first study between IGF2BP2 gene polymorphisms and type II diabetes in Turkish population.
Keywords: IGF2BP2, T2D, Polymorphism

Anahtar Kelimeler

IGF2BP2, T2D, Polymorphism

Proje Numarası

BAP-SBE TTB (DY) 2013-3 YL

Tip 2 Diyabet Hastalarında İnsülin Benzeri Büyüme Faktörü 2 mRNA Bağlayıcı Protein 2 Gen Polimorfizmlerinin Araştırılması

Öz

Amaç: Prevalansı farklı toplumlarda farklılık gösteren Tip 2 Diyabet (T2D) multifaktöriyel bir hastalıktır. T2D, genetik veya çevresel faktörler tarafından tetiklenen glikoz metabolizması bozukluklarından kaynaklanan bir grup klinik sendromu tanımlamaktadır. İnsülin benzeri büyüme faktörü 2 mRNA bağlayıcı protein 2 (IGF2BP2) geni, insülin sinyal yolağına katılır ve insülin salgılanmasında rol oynar. Bu gendeki polimorfizmlerin (rs1470579 ve rsS4402960) diyabet riskini kısmen artırdığı bildirilmiştir. Bu çalışmanın amacı, Türk toplumunda rs4402960 ve rs1470579 IGF2BP2 varyantlarının T2D ile olan ilişkilerini araştırmaktır.
Gereç ve Yöntem: IGF2BP2 geninin iki SNP'sini, rs1470579 ve rs4402960, 100 sağlıklı bireyde ve 100 hastada genotiplendirdik. DNA izolasyonu hasta ve sağlıklı gruplardan alınan periferik kan örnekleri üzerinde gerçekleştirilmiştir. Her bireyin IGF2BP2 genine ait rs1470579 ve rs4402960 polimorfizmlerinin moleküler analizi Real-Time PCR (Applied Biosystems) yöntemi kullanılarak gerçekleştirilmiştir. IGF2BP2 polimorfizmlerinin genotip ve alel sıklığı ile T2D arasındaki ilişkiler "Ki-kare" veya "Olabilirlik oranı" testleri ile incelenmiştir.
Bulgular: Genotip ve alel dağılımları sonucunda; IGF2BP2 rs1470579 (A/C) gen polimorfizmi için tip II diyabet hastaları ile kontrol grubu arasında ilişki bulunmuştur (p=0.0123). Hastalarda AC genotipinin sıklığı kontrol grubundan daha fazladır. Ancak, IGF2BP2 rs4402960 (G/T) gen polimorfizmleri için T2D hastaları ve kontrol grubu arasında genotip dağılımı açısından istatistiksel olarak anlamlı bir fark bulunmamıştır. TT ve GG+GT genotip dağılımı için hastalar ve kontrol grubu arasında bir ilişki bulunmamıştır (p=0.8847).
Sonuçlar: Sonuçlar, IGF2BP2 geni rs1470579 ve rs4402960 polimorfizmlerinin Türk toplumunda T2D ile ilişkili olduğunu göstermiştir (OR = 2.002, %95 CI 1.170-3.426, p < 0.05; OR = 1.879, %95 CI 1.110-3.182, p < 0.05). Bu çalışma, Türk toplumunda IGF2BP2 gen polimorfizmleri ile T2D arasındaki ilk çalışmadır.
Anahtar Kelimeler: IGF2BP2, T2D, Polimorfizm

Anahtar Kelimeler

IGF2BP2, Tip 2 Diyabet, Polimorfizm

Etik Beyan

Bu çalışma Mersin Üniversitesi Rektörlüğü Klinik Araştırmalar Etik Kurulu tarafından 22.11.2012 tarih ve 2012/370 karar sayısı ile onaylanmıştır.

Destekleyen Kurum

Mersin Üniversitesi Rektörlüğü Bilimsel Araştırma Projeler Birimi tarafından BAP-SBE TTB (DY) 2013-3 YL kodlu proje olarak desteklenmiştir.

Proje Numarası

BAP-SBE TTB (DY) 2013-3 YL

Teşekkür

Yüksek lisans eğitimim boyunca, her konuda bana yardımcı olan ve her zaman bana destek olan danışman hocam Prof. Dr. Nurcan Aras’a teşekkür ederim

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