COMPARISON OF DIFFERENT SOLID PHASE MICROEXTRACTION FIBERS IN EXTRACTION OF VOLATILE COMPOUNDS FROM ADIPOSE TISSUE

Öz

The volatile compounds (VCs) in headspace of adipose tissues (perirenal, tail, caul) were extracted by solid-phase microextraction (SPME) fiber coated with carboxen/ polydimethylsiloxane (CAR/PDMS) or divinylbenzene/ carboxen/ polydimethylsloxane (DVB/CAR/PDMS). In extraction with CAR/PDMS coating compared to DVB/CAR/PDMS, more VCs were obtained in perirenal (53 versus 41), tail (51 vs 47) and caul (35 vs 33) fats. Increased retention index for aldehydes increased the adsorption on DVB/CAR/PDMS. This fiber also provided a good yield for carboxylic acids. CAR/PDMS fiber could be recommended for VCs from adipose tissues if the goal is to identify aldehydes, especially fatty acid oxidation products and low molecular weight hydrocarbons, as well as greater number of VCs. However, in case of targeting carboxylic acids, esters, and also high molecular weight aldehydes, DVB/CAR/PDMS could be utilized due to its high affinity for mentioned compounds. Furthermore, the optimum extraction time should be studied to determine the time for VCs to reach equilibrium.

Anahtar Kelimeler

• Tail fat
• aldehydes
• polydimethylsiloxane
• linoleic acid

YAĞ DOKUSUNDAN UÇUCU BİLEŞENLERİN EKSTRAKSİYONUNDA FARKLI KATI FAZ MİKROEKSTRAKSİYON FİBERLERİNİN KARŞILAŞTIRILMASI

Öz

Adipoz dokuların (böbrek, kuyruk ve gömlek yağ) tepe boşluğundan uçucu bileşenlerin ekstraksiyonu, farklı adsorbant karboksen/ polidimetilsiloksan, (CAR/PDMS) ya da divinilbenzen/ karboksen/ polidimetilsiloksan (DVB/CAR/PDMS) kaplamalı katı faz mikroekstraksiyon fiberleri ile gerçekleştirilmiştir. DVB/CAR/PDMS ile karşılaştırıldığında CAR/PDMS kaplamalı fiber ile ekstraksiyonda böbrek (53’e 41), kuyruk (51’e 47) ve gömlek (35’e 33) yağlarda daha fazla sayıda uçucu bileşen belirlenmiştir. Aldehitlerin alıkonma indeksinin artması, DVB/CAR/PDMS’e adsorpsiyonu artırmıştır. Bu fiber karboksilik asitler için de etkili bir affinite göstermiştir. Adipoz dokularda, özellikle yağ asidi oksidasyon ürünü aldehitlerin, düşük molekül ağırlıklı hidrokarbonların ve ayrıca daha fazla uçucu bileşenin tanımlanması için CAR/PDMS fiberi önerilebilir. Ayrıca, karboksilli asitler, esterler ve yüksek molekül ağırlıklı aldehitlerin belirlenmesinde ise söz edilen bileşenlere affinitesinin yüksek olması nedeniyle DVB/CAR/PDMS kullanılabilir. Bununla birlikte uçucu bileşenlerin dengeye ulaşma zamanını belirlemek için optimum ekstraksiyon sürelerinin araştırılması gerekli olabilmektedir.

Anahtar Kelimeler

• Kuyruk yağı
• aldehitler
• polidimetilsiloksan
• linoleik asit

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