THE IMPACT OF RAISIN CONSUMPTION ON QUERCETIN BIOAVAILABILITY: AN IN VIVO APPROACH IN VIVO

Öz

Raisins are a nutrient-dense food known for their high content of dietary fiber, antioxidants, and bioactive compounds, including quercetin, which exists predominantly in the form of quercetin glycosides, enhancing their potential health benefits. This study investigates the impact of dietary matrix on quercetin bioavailability by analyzing urinary excretion following consumption of raisins. Employing advanced LC/MS techniques, the study quantified quercetin and its metabolites to evaluate how whole foods influence the absorption and metabolic processing of dietary polyphenols. Initial results indicated a significant increase in urinary quercetin excretion, with concentrations ranging from 21.8 µg/ml to 238.8 µg/ml among participants after consuming raisins. The study showed the role of the food matrix in enhancing quercetin bioavailability, suggesting that the complex interactions within whole foods like raisins could significantly influence the solubility, stability, and absorption of quercetin.

Anahtar Kelimeler

• Keywords: Raisins
• quercetin
• bioavailability
• excretion
• food matrix

KURU ÜZÜM TÜKETİMİNİN KUERSETİN BİYOYARARLANIMI ÜZERİNDEKİ ETKİSİ: IN VIVO YAKLAŞIM

Öz

Kuru üzüm yüksek oranda diyet lifi, antioksidan ve ağırlıklı olarak kuersetin glikozitleri formunda bulunan kuersetin de dahil olmak üzere farklı biyoaktif bileşikler içermektedir. İçerdiği bu bileşiklerden dolayı sağlık üzerine yararlı etkileri olduğu bilinmektedir. Bu çalışmada, kuru üzüm tüketimini takiben idrardaki kuersetin atılımı analiz edilerek diyet matrisinin kuersetinin biyoyararlanımı üzerindeki etkisi araştırılmıştır. Çalışmada gelişmiş LC/MS teknikleri kullanılarak, diyetteki gıdaların, polifenol emilimine ve metabolizmasına etki derecesini tespit etmek için idrardaki kuersetin ve metabolitlerinin miktarları belirlemiştir. Kuru üzüm tükettikten sonra idrarla kuersetin atılımında önemli bir artış olduğunu ve konsantrasyonların 21.8 µg/ml ile 238.8 µg/ml arasında değiştiğini göstermiştir. Çalışma, kuersetinin biyoyararlanımını arttırmada gıda matrisinin rolünün önemini belirterek, kuru üzüm gibi işlenmemiş gıdalardaki karmaşık etkileşimlerin, kuersetinin çözünürlüğünü, stabilitesini ve emilimini önemli ölçüde etkileyebileceğini göstermektedir.

Anahtar Kelimeler

• Kuru üzüm
• kuersetin
• biyoyararlanım
• atılım
• besin matrisi

Kaynakça

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