YERLİ BİR ELMA İZOLATININ FONKSİYONEL ÖZELLİĞİ: ATIKLARIN DEĞERLENDİRİLMESİ YOLUYLA METSCHNIKOWIA PULCHERRIMA ELM-GS-3 TARAFINDAN PULKERİMİN ÜRETİMİ

Öz

Metschnikowia pulcherrima ELM-GS-3, hasar görmüş Granny Smith elmalarından izole edilmiş ve MALDI-TOF MS ile ITS dizileme yöntemleri kullanılarak tanımlanmıştır (%97.89 benzerlik, NCBI veritabanı). Pulcherrimin üretimi, FeCl₃ içeren besiyerinde bordo-kırmızı pigment oluşumu ve mikroskobik analiz ile doğrulanmıştır. Patates kabuğu, soğan kabuğu, karpuz kabuğu ekstreleri ve seyreltilmiş melas gibi gıda atıklarından türetilmiş besiyerleri, biyokütle ve pigment üretimi açısından değerlendirilmiştir. En yüksek biyokütle (9.78±0.1 g/L) ve pulcherrimin (7.63±0.6 g/L) üretimi soğan kabuğu ekstresinde gözlemlenmiş, bunu sırasıyla melas ve karpuz kabuğu takip etmiştir. FTIR analizi pigmentin demir içerdiğini doğrularken, SEM analizi amorf ve mikroporoz 3D bir yapı ortaya koymuştur. Pigmentin alkali çözeltide maksimum absorpsiyon dalga boyu 420 nm olarak belirlenmiş ve düşük çözünürlük profili, literatürde bildirilen stabilite özellikleriyle uyumlu bulunmuştur. Bu çalışma, gıda atıklarının pulcherrimin üretiminde potansiyelini ve M. pulcherrima ELM-GS-3’ün biyoteknolojik önemini ortaya koymaktadır.

Anahtar Kelimeler

• Metchnikowia pulcherrima
• pulkerimin
• soğan kabuğu
• atık
• fermantasyon
• ITS dizileme

A FUNCTIONAL PROPERTY OF A DOMESTIC APPLE ISOLATE: PULCHERRIMIN PRODUCTION BY METSCHNIKOWIA PULCHERRIMA ELM-GS-3 VIA WASTE VALORIZATION

Öz

Metschnikowia pulcherrima ELM-GS-3 was isolated from damaged Granny Smith apples and identified via MALDI-TOF MS and ITS sequencing (97.89% similarity, NCBI database). Pulcherrimin production was confirmed on FeCl3-supplemented media by maroon-red pigment formation and microscopic analysis. Food waste-derived media, including potato peel, onion skin, watermelon rind extracts, and diluted molasses, were evaluated for biomass and pigment production. Onion skin extract yielded the highest biomass (9.78±0.1 g/L) and pulcherrimin (7.63±0.6 g/L), followed by molasses and watermelon rind. FTIR analysis confirmed iron presence, while SEM revealed an amorphous microporous 3D structure. Absorbance peaked at 420 nm in alkali solution, consistent with low solubility except in alkaline conditions. The pigment’s low solubility profile, except in alkali, aligns with its stability characteristics observed in the literature. This study demonstrates the potential of food waste in pulcherrimin production and the biotechnological relevance of M. pulcherrima ELM-GS-3.

Anahtar Kelimeler

• Metchnikowia pulcherrima
• pulcherrimin
• waste
• fermentation
• ITS sequencing

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