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IN VITRO MINERAL BIOACCESSIBILITY AND CONTRIBUTION TO DIETARY MINERAL INTAKE OF COMMERCIALLY AVAILABLE BREAD TYPES IN TÜRKİYE

Yıl 2026, Cilt: 51 Sayı: 2 , 344 - 366 , 31.03.2026
https://izlik.org/JA58JK24PB

Öz

This study evaluated the mineral composition, in vitro mineral bioaccessibility and estimated contribution to dietary mineral intake of commonly consumed breads in Türkiye. Five bread types (white wheat, whole wheat, sourdough wheat, buckwheat and einkorn) were purchased from five independent commercial brands. Moisture, ash and protein were determined by standard methods, while K, Mg, Ca, Na, Fe, Mn and Zn were quantified by I CP-OES a fter microwave digestion. A m odified static INFOGEST protocol was applied to assess in vitro gastrointestinal digestion and mineral bioaccessibility. Buckwheat and einkorn breads showed the highest Mg, Fe, Mn and Zn contents, whereas white wheat bread contained the highest Na. Sourdough bread exhibited the greatest bioaccessibility for Fe, Mn and Zn. Based on EFSA reference values, a 250 g portion of bread provided approximately 14-17% of the adequate intake for K, but ≤2% of Ca and limited Zn, while sourdough and buckwheat breads substantially improved bioaccessible Fe and Mn supply.

Kaynakça

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TÜRKİYE'DE YAYGIN OLARAK TÜKETİLEN EKMEK ÇEŞİTLERİNİN İN VİTRO MİNERAL BİYOERİŞİLEBİLİRLİĞİ VE DİYET MİNERAL ALIMINA KATKISI

Yıl 2026, Cilt: 51 Sayı: 2 , 344 - 366 , 31.03.2026
https://izlik.org/JA58JK24PB

Öz

Bu çalışma, Türkiye’de yaygın olarak tüketilen ekmeklerin mineral kompozisyonu, in vitro mineral biyoerişilebilirliği ve önerilen günlük alıma katkısını değerlendirmeyi amaçlamıştır. Beyaz buğday, tam buğday, ekşi mayalı buğday, karabuğday ve siyez ekmekleri, her biri beş farklı ticari üreticiden temin edilmiştir. Nem, kül ve protein içerikleri standart yöntemlerle belirlenmiş; K, Mg, Ca, Na, Fe, Mn ve Zn konsantrasyonları mikrodalga destekli asit sindirimi sonrası ICP-OES ile analiz edilmiştir. Mineral biyoerişilebilirliğini belirlemek için ekmek matrisi için uyarlanan statik INFOGEST protokolü kullanılmıştır. Karabuğday ve siyez ekmekleri Mg, Fe, Mn ve Zn bakımından en zengin ürünler olurken, en yüksek Na içeriği beyaz buğday ekmeğinde saptanmıştır. Ekşi mayalı ekmek, özellikle Fe, Mn ve Zn için en yüksek biyoerişilebilirlik yüzdelerini göstermiştir. EFSA referans değerlerine göre 250 g ekmek porsiyonu potasyum yeterli alımının yaklaşık %14-17’sini karşılarken, kalsiyuma ve kısmen çinkoya sınırlı katkı sağlamaktadır.

Kaynakça

  • Adrogué, H. J., Madias, N. E. (2007). Sodium and potassium in the pathogenesis of hypertension. The New England Journal of Medicine, 356(19), 1966-1978. https://doi.org/10.1056/NEJMra064486
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  • Alhagri, I. A., Albeshry, M. (2023). Microwave-Assisted Digestion Using Dilute Nitric Acid and Hydrogen Peroxide for Multielement Determination in Wheat Flour by ICP-MS. Journal of Chemistry, 2023(1), 2349838. https://doi.org/10.1155/2023/2349838
  • Alkay, Z., Alkay, R., Dertli, E., Kökten, K., Durak, M. Z. (2023). Rheological, Textural and Physicochemical Properties of Buckwheat Sourdough Bread Prepared With Different Lactic Acid Bacteria Strains. Journal of Microbiology, Biotechnology and Food Sciences, 12(5). https://doi.org/10.55251/jmbfs.5643
  • Alkay, Z., Falah, F., Cankurt, H., Dertli, E. (2024). Exploring the Nutritional Impact of Sourdough Fermentation: Its Mechanisms and Functional Potential. Foods, 13(11). https://doi.org/10.3390/foods13111732
  • Alvarez-Jubete, L., Arendt, E. K., Gallagher, E. (2010). Nutritive value of pseudocereals and their increasing use as functional gluten-free ingredients. Trends in Food Science & Technology, 21(2), 106-113. https://doi.org/10.1016/j.tifs.2009.10.014
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  • Comunian, T. A., Freitas, D., Drouin, G., Maudhuit, A., Roelens, G., Poncelet, D., Drusch, S., & Brodkorb, A. (2024). Semi-dynamic in vitro digestion of sourdough bread enriched with flaxseed oil coacervates. Food Structure, 40, 100371. https://doi.org/10.1016/j. foostr.2024.100371
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  • Dewettinck, K., Van Bockstaele, F., Kühne, B., Van de Walle, D., Courtens, T. M., Gellynck, X. (2008). Nutritional value of bread: Influence of processing, food interaction and consumer perception. Journal of Cereal Science, 48(2), 243-257. https://doi.org/10.1016/j. jcs.2008.01.003
  • Echavarría, J. A. C., El Hajj, S., Irankunda, R., Selmeczi, K., Paris, C., Udenigwe, C. C., & Canabady-Rochelle, L. (2024). Screening{,} separation and identification of metal-chelating peptides for nutritional{,} cosmetics and pharmaceutical applications. Food Funct., 15(7), 3300-3326. https://doi.org/10.1039/D3FO05765H
  • EFSA. (2013). Scientific Opinion on Dietary Reference Values for manganese. EFSA Journal, 11(11), 3419. https://doi.org/10.2903/j. efsa.2013.3419
  • EFSA. (2015). Dietary reference values: magnesium and phosphorus.
  • EFSA. (2016). Dietary reference values for potassium. EFSA Journal, 14(10), e04592. https://doi.org/10.2903/j.efsa.2016.4592
  • EFSA. (2017). Dietary Reference Values for nutrients Summary report. EFSA Supporting Publications, 14(12), e15121E. https://doi. org/10.2903/sp.efsa.2017.e15121
  • EFSA. (2019). Dietary reference values for sodium. EFSA Journal, 17(9), e05778. https://doi.org/10.2903/j.efsa.2019.5778
  • EL Houssni, I., Zahidi, A., Khedid, K., Hassikou, R. (2024). Review of processes for improving the bioaccessibility of minerals by reducing the harmful effect of phytic acid in wheat. Food Chemistry Advances, 4, 100568. https://doi.org/10.1016/j.focha.2023.100568
  • El, I., Zahidi, A., Khedid, K., Hassikou, R. (2024). Review of processes for improving the bioaccessibility of minerals by reducing the harmful effect of phytic acid in wheat. Food Chemistry Advances, 4(December 2023), 100568. https://doi.org/10.1016/j.focha.2023.100568
  • Emkey, R. D., Emkey, G. R. (2012). Calcium Metabolism and Correcting Calcium Deficiencies. Endocrinology and Metabolism Clinics, 41(3), 527-556. https://doi.org/10.1016/j.ecl.2012.04.019
  • Etcheverry, P., Grusak, M. A., Fleige, L. E. (2012). Application of in vitro bioaccessibility and bioavailability methods for calcium, carotenoids, folate, iron, magnesium, polyphenols, zinc, and vitamins B(6), B(12), D, and E. Frontiers in Physiology, 3, 317. https://doi. org/10.3389/fphys.2012.00317
  • Fărca₃, A. C., Socaci, S. A., Chi₃, M. S., Martínez-Monzó, J., García-Segovia, P., Becze, A., Török, A. I., Cadar, O., Coldea, T. E., & Igual, M. (2022). In Vitro Digestibility of Minerals and B Group Vitamins from Different Brewers’ Spent Grains. Nutrients, 14(17). https://doi. org/10.3390/nu14173512
  • Faria, M. A., Araújo, A., Pinto, E., Oliveira, C., Oliva-Teles, M. T., Almeida, A., Delerue-Matos, C., & Ferreira, I. M. P. L. V. O. (2018). Bioaccessibility and intestinal uptake of minerals from different types of home-cooked and ready-to-eat beans. Journal of Functional Foods, 50, 201-209. https://doi.org/10.1016/j.jff.2018.10.001
  • Fayaz, U., Dar, A. H., Kumar, N., Junaid, P. M., Shams, R., Khan, S. A. (2021). Formulations and quality characterization of low salt flat bread: Effects on functionality, rheological and sensory properties. Applied Food Research, 1(1), 100005. https://doi.org/10.1016/j. afres.2021.100005
  • Ferruzzi, M. G., Kruger, J., Mohamedshah, Z., Debelo, H., Taylor, J. R. N. (2020). Insights from in vitro exploration of factors influencing iron, zinc and provitamin A carotenoid bioaccessibility and intestinal absorption from cereals. Journal of Cereal Science, 96, 103126. https://doi.org/10.1016/j.jcs.2020.103126
  • Frontela, C., Ros, G., Martínez, C. (2011). Phytic acid content and “in vitro” iron, calcium and zinc bioavailability in bakery products: The effect of processing. Journal of Cereal Science, 54(1), 173-179. https://doi.org/10.1016/j.jcs.2011.02.015
  • Gibson, R. S., King, J. C., Lowe, N. (2016). A Review of Dietary Zinc Recommendations. Food and Nutrition Bulletin, 37(4), 443-460. https://doi.org/10.1177/0379572116652252
  • Gupta, R. K., Gangoliya, S. S. (2015). Reduction of phytic acid and enhancement of bioavailable micronutrients in food grains. 52(February), 676-684. https://doi.org/10.1007/s13197-013-0978-y
  • Hayta, M., Hendek Ertop, M. (2018). Evaluation of microtextural properties of sourdough wheat bread obtained from optimized formulation using scanning electron microscopy and image analysis during shelf life. Journal of Food Science and Technology, 55(1), 1-9. https://doi.org/10.1007/s13197-017-2823-1
  • Keçeli, A., Kaplan Evlice, A., Pehlivan, A., Şanal, T., Zencirci, N. (2021). Bread-making properties of einkorn wheat (Triticum monococcum ssp. monococcoum) and its suitability for whole wheat bread production. Cereal Research Communications, 49(4), 625-637. https://doi.org/10.1007/s42976-021-00146-0
  • Kippler, M., Oskarsson, A. (2024). Manganese - a scoping review for Nordic Nutrition Recommendations 2023. Food & Nutrition Research, 68. https://doi.org/10.29219/fnr.v68.10367
  • Klepacka, J., Najda, A., Klimek, K. (2020). Effect of Buckwheat Groats Processing on the Content and Bioaccessibility of Selected Minerals. Foods (Basel, Switzerland), 9(6). https://doi.org/10.3390/foods9060832
  • Kugler, S., Hristov, H., Blaznik, U., Hribar, M., Hafner, E., Kušar, A., Pravst, I. (2025). Insights into the salt levels in bread offers in Slovenia: trends and differences. Frontiers in Nutrition, Volume 11-2024. https://doi.org/10.3389/fnut.2024.1473362
  • Latha Rani, R., Kulkarni, U. N., Biradar, S. (2022). Essential mineral composition of buckwheat (Fagopyrum esculentum) varieties for nutrition security.
  • Longin, C. F. H., Afzal, M., Pfannstiel, J., Bertsche, U., Melzer, T., Ruf, A., & Rodehutscord, M. (2023). Mineral and Phytic Acid Content as Well as Phytase Activity in Flours and Breads Made from Different Wheat Species. International Journal of Molecular Sciences, 24(3). https://doi.org/10.3390/ijms24032770
  • Lopez, A., Cacoub, P., Macdougall, I. C., Peyrin-Biroulet, L. (2016). Iron deficiency anaemia. The Lancet, 387(10021), 907-916. https://doi.org/10.1016/S0140-6736(15)60865-0
  • Lopez, H. W., Duclos, V., Coudray, C., Krespine, V., Feillet-Coudray, C., Messager, A., Demigné, C., & Rémésy, C. (2003). Making bread with sourdough improves mineral bioavailability from reconstituted whole wheat flour in rats. Nutrition (Burbank, Los Angeles County, Calif.), 19(6), 524-530. https://doi.org/10.1016/s0899-9007(02)01079-1
  • Lucretia Ifeoma, B., Nkechi Juliet T., E. (2021). Physical Characteristics and Mineral Composition of Bread Produced from Blends of Wheat and Defatted/Undefatted Cashew Kernel Flours. Asian Food Science Journal, 20(2 SE-Original Research Article), 1-11. https://doi. org/10.9734/afsj/2021/v20i230259
  • Magallanes-López, A. M., Hernandez-Espinosa, N., Velu, G., Posadas-Romano, G., Ordoñez-Villegas, V. M. G., Crossa, J., & Guzmán, C. (2017). Variability in iron, zinc and phytic acid content in a worldwide collection of commercial durum wheat cultivars and the effect of reduced irrigation on these traits. Food Chemistry, 237, 499-505. https://doi.org/10.1016/j.foodchem.2017.05.110
  • Minekus, M., Alminger, M., Alvito, P., Ballance, S., Bohn, T., Bourlieu, C., &, Brodkorb, A. (2014). A standardised static in vitro digestion method suitable for food-an international consensus. Food and Function, 5(6), 1113 - 1124. https://doi.org/10.1039/c3fo60702j
  • Mohajan, S., Munna, M., Orchy, T., Hoque, M., Farzana, T. (2019). Buckwheat flour fortified bread. Bangladesh Journal of Scientific and Industrial Research, 54(4), 347-356. https://doi.org/10.3329/bjsir. v54i4.44569
  • Morrissey, E., Giltinan, M., Kehoe, L., Nugent, A. P., McNulty, B. A., Flynn, A., & Walton, J. (2020). Sodium and Potassium Intakes and Their Ratio in Adults (18-90 y): Findings from the Irish National Adult Nutrition Survey. Nutrients, 12(4). https://doi.org/10.3390/nu12040938
  • Nargesi, M. H., Kheiralipour, K. (2025). Estimating ash content in wheat flour using visible-near infrared hyperspectral imaging and machine learning methods. LWT, 234, 118591. https://doi. org/10.1016/j.lwt.2025.118591
  • Navarro, J. L., López, M. S., Salvucci, E., León, A. E., Steffolani, M. E. (2025). Chemical and Nutritional Characterization of Sourdoughs Made with Sprouted and Unsprouted Whole-Wheat Flour and Their Effects on the Technological Quality of Bread. Foods, 14(16). https://doi.org/10.3390/foods14162805
  • Nsabimana, S., Ismail, T., Lazarte, C. E. (2024). Enhancing iron and zinc bioavailability in maize (Zea mays) through phytate reduction: the impact of fermentation alone and in combination with soaking and germination. Frontiers in Nutrition, 11(December), 1-13. https://doi.org/10.3389/fnut.2024.1478155
  • Öğünç, Y. T., Aytekin, Ö., Rakıcıoğlu, N. (2026). Enhancing the nutritional profile of refined wheat bread with cereal and pulse flours: assessment of phytate content and mineral bioaccessibility. European Food Research and Technology, 252(2), 44.
  • Pasricha, S.-R., Tye-Din, J., Muckenthaler, M. U., Swinkels, D. W. (2021). Iron deficiency. The Lancet, 397(10270), 233-248. https://doi. org/10.1016/S0140-6736(20)32594-0
  • Păucean, A., Șerban, L.-R., Chiș, M. S., Mureșan, V., Pușcaș, A., Man, S. M., & Pop, A. (2024). Nutritional composition, in vitro carbohydrates digestibility, textural and sensory characteristics of bread as affected by ancient wheat flour type and sourdough fermentation time. Food Chemistry: X, 22, 101298. https://doi.org/10.1016/j. fochx.2024.101298
  • Piergiovanni, A. R., Rizzi, R., Pannacciulli, E., Gatta, C. Della. (1997). Mineral composition in hulled wheat grains: a comparison between emmer (Triticum dicoccon Schrank) and spelt (T. spelta L.) accessions. International Journal of Food Sciences and Nutrition, 48(6), 381-386.
  • Pravina, P., Sayaji, D., Avinash, M. (2013). Calcium and its role in human body. International Journal of Research in Pharmaceutical and Biomedical Sciences, 4(2), 659-668.
  • Przybylski, R., Gruczyyska, E. (2009). A Review of Nutritional and Nutraceutical Components of Buckwheat. The European Journal of Plant Science and Biotechnology, 3(1), 10-22.
  • Renzetti, S., Theunissen, M., Horrevorts, K. (2021). A Systematic Comparison of the Intrinsic Properties of Wheat and Oat Bran Fractions and Their Effects on Dough and Bread Properties: Elucidation of Chemical Mechanisms, Water Binding, and Steric Hindrance. Foods, 10(10). https://doi.org/10.3390/foods10102311
  • Ruan, R., Shiroud Heidari, B., Chen, P., Gao, J., Chen, Z., Chen, L., & Zheng, M. (2024). Converting ocean nacre into bone mineral matrix composite for bone regeneration- in vitro and in vivo studies. Materials & Design, 245, 113271. https://doi.org/10.1016/j. matdes.2024.113271
  • Ruibal-Mendieta, N. L., Delacroix, D. L., Mignolet, E., Pycke, J. M., Marques, C., Rozenberg, R., & Larondelle, Y. (2005). Spelt (Triticum aestivum ssp. spelta) as a source of breadmaking flours and bran naturally enriched in oleic acid and minerals but not phytic acid. Journal of Agricultural and Food Chemistry, 53(7), 2751-2759. https://doi.org/10.1021/jf048506e
  • Sezer, B., Ozturk, M., Ayvaz, H., Apaydın, H., Boyaci, I. H. (2022). Laser-induced breakdown spectroscopy as a reliable analytical method for classifying commercial cheese samples based on their cooking/stretching process. Food Chemistry, 390, 132946. https://doi. org/10.1016/j.foodchem.2022.132946
  • Ting, S. R., Loh, S. P. (2016). In vitro bioaccessibility of calcium, iron and zinc from breads and bread spreads. International Food Research Journal, 23(5).
  • Tokarczyk, J., Koch, W. (2025). Dietary Zn—Recent Advances in Studies on Its Bioaccessibility and Bioavailability. Molecules, 30(13). https://doi.org/10.3390/molecules30132742
  • Wang, Z., Wang, L. (2024a). Grain & Oil Science and Technology Impact of sourdough fermentation on nutrient transformations in cereal-based foods : Mechanisms , practical applications , and health implications. Grain & Oil Science and Technology, 7(2), 124-132. https://doi.org/10.1016/j.gaost.2024.03.001
  • Wang, Z., Wang, L. (2024b). Impact of sourdough fermentation on nutrient transformations in cereal-based foods: Mechanisms, practical applications, and health implications. Grain & Oil Science and Technology, 7(2), 124-132. https://doi.org/10.1016/j.gaost.2024.03.001
  • Zimmermann, M. B., Hurrell, R. F. (2007). Nutritional iron deficiency. The Lancet, 370(9586), 511-520. https://doi.org/10.1016/S0140-6736(07)61235-5
  • Żuk-Gołaszewska, K., Majewska, K., Tyburski, J., Gołaszewski, J. (2022). Nutritional properties of organic spelt wheats in different growth stages and the resulting flours. Journal of Elementology, 27(3), 645-662.
Toplam 74 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Gıda ve Beslenme Dengesi, Gıda Mühendisliği
Bölüm Araştırma Makalesi
Yazarlar

Hakan Apaydin 0000-0001-8507-6212

Gönderilme Tarihi 23 Kasım 2025
Kabul Tarihi 16 Şubat 2026
Yayımlanma Tarihi 31 Mart 2026
DOI https://doi.org/10.15237/gida. GD25141
IZ https://izlik.org/JA58JK24PB
Yayımlandığı Sayı Yıl 2026 Cilt: 51 Sayı: 2

Kaynak Göster

APA Apaydin, H. (2026). IN VITRO MINERAL BIOACCESSIBILITY AND CONTRIBUTION TO DIETARY MINERAL INTAKE OF COMMERCIALLY AVAILABLE BREAD TYPES IN TÜRKİYE. Gıda, 51(2), 344-366. https://doi.org/10.15237/gida. GD25141
AMA 1.Apaydin H. IN VITRO MINERAL BIOACCESSIBILITY AND CONTRIBUTION TO DIETARY MINERAL INTAKE OF COMMERCIALLY AVAILABLE BREAD TYPES IN TÜRKİYE. GIDA. 2026;51(2):344-366. doi:10.15237/gida. GD25141
Chicago Apaydin, Hakan. 2026. “IN VITRO MINERAL BIOACCESSIBILITY AND CONTRIBUTION TO DIETARY MINERAL INTAKE OF COMMERCIALLY AVAILABLE BREAD TYPES IN TÜRKİYE”. Gıda 51 (2): 344-66. https://doi.org/10.15237/gida. GD25141.
EndNote Apaydin H (01 Mart 2026) IN VITRO MINERAL BIOACCESSIBILITY AND CONTRIBUTION TO DIETARY MINERAL INTAKE OF COMMERCIALLY AVAILABLE BREAD TYPES IN TÜRKİYE. Gıda 51 2 344–366.
IEEE [1]H. Apaydin, “IN VITRO MINERAL BIOACCESSIBILITY AND CONTRIBUTION TO DIETARY MINERAL INTAKE OF COMMERCIALLY AVAILABLE BREAD TYPES IN TÜRKİYE”, GIDA, c. 51, sy 2, ss. 344–366, Mar. 2026, doi: 10.15237/gida. GD25141.
ISNAD Apaydin, Hakan. “IN VITRO MINERAL BIOACCESSIBILITY AND CONTRIBUTION TO DIETARY MINERAL INTAKE OF COMMERCIALLY AVAILABLE BREAD TYPES IN TÜRKİYE”. Gıda 51/2 (01 Mart 2026): 344-366. https://doi.org/10.15237/gida. GD25141.
JAMA 1.Apaydin H. IN VITRO MINERAL BIOACCESSIBILITY AND CONTRIBUTION TO DIETARY MINERAL INTAKE OF COMMERCIALLY AVAILABLE BREAD TYPES IN TÜRKİYE. GIDA. 2026;51:344–366.
MLA Apaydin, Hakan. “IN VITRO MINERAL BIOACCESSIBILITY AND CONTRIBUTION TO DIETARY MINERAL INTAKE OF COMMERCIALLY AVAILABLE BREAD TYPES IN TÜRKİYE”. Gıda, c. 51, sy 2, Mart 2026, ss. 344-66, doi:10.15237/gida. GD25141.
Vancouver 1.Hakan Apaydin. IN VITRO MINERAL BIOACCESSIBILITY AND CONTRIBUTION TO DIETARY MINERAL INTAKE OF COMMERCIALLY AVAILABLE BREAD TYPES IN TÜRKİYE. GIDA. 01 Mart 2026;51(2):344-66. doi:10.15237/gida. GD25141

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