COĞRAFİ KÖKENİN TÜRK PROPOLİSİNİN FİZİKOKİMYASAL ÖZELLİKLERİ, FENOLİK İÇERİĞİ, ANTİOKSİDAN KAPASİTESİ VE GASTROİNTESTİNAL BİYOERİŞİLEBİLİRLİĞİ ÜZERİNDEKİ ETKİSİ

Öz

Bu çalışmada, Türkiye’nin farklı bölgelerinden toplanan propolis örnekleri nem içeriği, renk özellikleri, toplam fenolik madde (TFM) miktarı ve toplam antioksidan kapasite (TAK) açısından karakterize edilmiş, ayrıca biyoaktif bileşiklerin in-vitro gastrointestinal sindirim sırasındaki biyoerişilebilirliği değerlendirilmiştir. Örneklerin nem içeriği 3,57–6,30 g/100 g arasında değişmiş ve kabul edilebilir kalite sınırları içerisinde bulunmuştur. Renk parametreleri, örnekler arasında önemli bölgesel farklılıklar ortaya koymuştur. Simüle edilmiş gastrointestinal sindirim, hem TFM hem de TAK değerlerini önemli düzeyde etkilemiştir (p<0,05). Başlangıç TFM değerleri 2048,91–6715,91 mg GAE/100 g kuru madde arasında değişirken, mide fazında %81,98–94,59 oranında azalmış, bağırsak fazı sonrasında ise %3,18–170,72 oranında artış göstermiştir. TAK bakımından, CUPRAC değerleri sindirim sürecinde önemli ölçüde artarken, DPPH ve FRAP değerleri genel olarak azalmıştır. Sonuç olarak, coğrafi kökenin propolisin fizikokimyasal özellikleri, biyoaktif potansiyeli ve gastrointestinal stabilitesi üzerinde belirleyici olduğu saptanmıştır. Elde edilen bulgular, kökene dayalı karakterizasyon, standardizasyon ve katma değerli fonksiyonel propolis ürünlerinin geliştirilmesi için önemli bilgiler sunmaktadır. Anahtar kelimeler: Propolis, fenolikler, antioksidan, in-vitro gastrointestinal sindirim

Anahtar Kelimeler

• Propolis
• fenolikler
• antioksidan
• in vitro gastrointestinal sindirim

INFLUENCE OF GEOGRAPHICAL ORIGIN ON PHYSICOCHEMICAL PROPERTIES, PHENOLIC CONTENT, ANTIOXIDANT CAPACITY, AND GASTROINTESTINAL BIOACCESSIBILITY OF TURKISH PROPOLIS

Öz

This study aimed to characterize propolis samples from different regions of Türkiye in terms of moisture content, color, total phenolic content (TPC), and total antioxidant capacity (TAC), and to assess the bioaccessibility of bioactives cduring in-vitro gastrointestinal digestion. Moisture ranged between 3.57 and 6.30 g/100 g, within acceptable quality limits, while color revealed significant regional variability. Simulated digestion affected both TPC and TAC (p < 0.05). Initial TPC varied between 2048.91 and 6715.91 mg GAE/100g dm. TPC decreased markedly during gastric phase (81.98–94.59%) but increased after intestinal digestion (3.18–170.72%). In terms of TAC, CUPRAC increased substantially during gastric (74.89–188.47%) and intestinal digestion (155.82–328.38%), whereas DPPH and FRAP generally decreased, reflecting differences in the stability and reactivity of antioxidants. Geographical origin influenced the physicochemical and bioactive properties and gastrointestinal stability. These findings provide valuable insights for origin-based characterization, standardization, and the development of value-added functional propolis products. Key words: Propolis; phenolics, antioxidant, in-vitro gastrointestinal digestion

Anahtar Kelimeler

• Propolis
• phenolics
• antioxidant
• in vitro gastrointestinal sindirim

Kaynakça

1. Abduh, M. Y., Shafitri, T. R., Elfahmi. (2024). Chemical profiling, bioactive compounds, antioxidant, and anti-inflammatory activities of Indonesian propolis extract produced by Tetragonula laeviceps. Heliyon, 10(19): e38736, doi: 10.1016/j.heliyon.2024.e38736.
2. Aboulghazi, A., Touzani, S., Fadil, M., Lyoussi, B. (2022). Physicochemical characterization and in vitro evaluation of the antioxidant and anticandidal activities of Moroccan propolis. Veterinary World, 15(2): 341–349, doi: 10.14202/vetworld.2022.341-349.
3. Altuntaş, Ü., Güzel, İ., Özçelik, B. (2023). Phenolic constituents, antioxidant and antimicrobial activity and clustering analysis of propolis samples based on PCA from different regions of Anatolia. Molecules, 28(3): 1121, doi: 10.3390/molecules28031121.
4. Alves, G., Lobo, L. A., Domingues, R. M. C. P., Monteiro, M., Perrone, D. (2021). Bioaccessibility and gut metabolism of free and melanoidin-bound phenolic compounds from coffee and bread. Frontiers in Nutrition, 8: 708928, doi: 10.3389/fnut.2021.708928.
5. AOAC. (2023). Official Methods of Analysis. 13th ed. Association of Official Analytical Chemists, Gaithersburg, MD, USA.
6. Apak, R., Güçlü, K., Özyürek, M., Çelik, S. E. (2008). Mechanism of antioxidant capacity assays and the CUPRAC (cupric ion reducing antioxidant capacity) assay. Microchimica Acta, 160(4): 413–419, doi: 10.1007/s00604-007-0777-0.
7. Aroufai, İ. A., Sabuncu, M., Dülger Altıner, D., Sahan, Y. (2022). Antioxidant properties and bioaccessibility of coffee beans and their coffee silverskin grown in different countries. Journal of Food Measurement and Characterization, 16(3): 1873–1888, doi: 10.1007/s11694-022-01325-5.
8. Arslan, M., Sevgiler, Y., Güven, C., Murathan, Z. T., Erbil, N., Yıldırım, D., Büyükleyla, M., Karadaş, Ş., Çelik, R., Rencüzoğulları, E. (2021). Chemical and biological characteristics of propolis from Apis mellifera caucasica from the Ardahan and Erzurum provinces of Türkiye: A comparative study. Archives of Industrial Hygiene and Toxicology, 72(1): 37–46, doi: 10.2478/aiht-2021-72-3492.

9. Bankova, V., Popova, M., Trusheva, B. (2018). The phytochemistry of the honeybee. Phytochemistry, 155: 1–11, doi: 10.1016/j.phytochem.2018.07.007.
10. Benzie, I. F. F., Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239(1): 70–76, doi: 10.1006/abio.1996.0292.
11. Bitencourt, B. S., Guedes, J. S., Saliba, A. S. M. C., Alencar, S. M., Sartori, A. G. O., Maniglia, B. C., Augusto, P. E. D. (2025). Modified wheat starch for the development of 3D-printed red propolis gels for dysphagic diets: Phenolic content and bioactivities during in vitro gastrointestinal digestion. International Journal of Biological Macromolecules, 318(4): 145240, doi: 10.1016/j.ijbiomac.2025.145240.
12. Boonmee, T., Sinpoo, C., Wongthaveethong, L., Chuesaard, T., Pukklay, P., Jakngern, T., Chaimanee, V. (2026). Enhanced extraction of antioxidant compounds from propolis using non-thermal plasma pretreatment and ultrasonic-assisted methods. LWT - Food Science and Technology, 241: 119082, doi: 10.1016/j.lwt.2026.119082.
13. Brodkorb, A., Egger, L., Alminger, M., Alvito, P., Assunção, R., Ballance, S., Bohn, T., Bourlieu-Lacanal, C., Boutrou, R., Carrière, F., Clemente, A., Corredig, M., Dupont, D., Dufour, C., Edwards, C., Golding, M., Karakaya, S., Kirkhus, B., Le Feunteun, S., Lesmes, U., Macierzanka, A., Mackie, A., Martins, C., Marze, S., McClements, D. J., Ménard, O., Minekus, M., Portmann, R., Santos, C. N., Souchon, I., Singh, R. P., Vegarud, G. E., Wickham, M. S. J., Weitschies, W., Recio, I. (2019). INFOGEST static in vitro simulation of gastrointestinal food digestion. Nature Protocols, 14(4): 991–1014, doi: 10.1038/s41596-018-0119-1.
14. Berretta, A. A., Arruda, C., Miguel, F. G., Baptista, N., Nascimento, A. P., Marquele-Oliveira, F., Hori, J. I., Barud, H. S., Damaso, B., Ramos, C., Ferreira, R., Bastos, J. K. (2017). Functional properties of Brazilian propolis: From chemical composition until the market. A. M. Grumezescu, A. M. Holban (Eds.), Superfood and Functional Food: An Overview of Their Processing and Utilization (pp. 55–98). IntechOpen, doi: 10.5772/65932.
15. Castaldo, L., Narváez, A., Izzo, L., Graziani, G., Ritieni, A. (2020). In vitro bioaccessibility and antioxidant activity of coffee silverskin polyphenolic extract and characterization of bioactive compounds using UHPLC-Q-Orbitrap HRMS. Molecules, 25(9): 2132, doi: 10.3390/molecules25092132.
16. Cetin, A., Bakir, S., Ozdal, T., Capanoglu, E. (2026). Investigation of antioxidant properties of propolis products collected from different regions. International Journal of Molecular Sciences, 27(2): 1046, doi: 10.3390/ijms27021046.
17. Cheng, K., Dong, W., Long, Y., Zhao, J., Hu, R., Zhang, Y., Zhu, K. (2019). Evaluation of the impact of different drying methods on the phenolic compounds, antioxidant activity, and in vitro digestion of green coffee beans. Food Science and Nutrition, 7(3): 1084–1095, doi: 10.1002/fsn3.953.
18. Çelemli, Ö. G. (2015). Chemical classification of propolis samples collected from different regions of Turkey in geographical region base. Hacettepe Journal of Biology and Chemistry, 43(1): 49–57, doi: 10.15671/HJBC.20154311204.
19. Deniz, Z., Suna, S. (2025). Unlocking the potential of elderberry (Sambucus nigra L.) fruit leather: Optimization of production and evaluation of bioactive content and bioaccessibility using response surface methodology. Food Chemistry, 493: 146046, doi: 10.1016/j.foodchem.2025.146046.
20. Erdogan, Ü. (2023). Ultrasonic assisted propolis extraction: Characterization by ATR-FTIR and determination of its total antioxidant capacity and radical scavenging ability. International Journal of Secondary Metabolite, 10(2): 231–239, doi: 10.21448/ijsm.1167773.
21. Ghumro, T., Memon, S. Q., Mallah, A., Palabiyik, I. M., Solangi, A. R., Fatima, A. (2025). Design of experiment for extraction optimization and antioxidant assay studies of biologically active compounds of propolis. Journal of Apicultural Research, 64(4): 1163–1171, doi: 10.1080/00218839.2024.2394744.
22. Grassi, G., Capasso, G., Gambacorta, E., Perna, A. M. (2023). Chemical and functional characterization of propolis collected from different areas of South Italy. Foods, 12(18): 3481, doi: 10.3390/foods12183481.
23. Guerrero, M. D. C. L., Hortmann, A. B., Bodnar, L. F., Pucholobek, G., Quinaia, S. P., Sierra, A. F., Torres, Y. R. (2026). Impact of in vitro static digestion of geopropolis and propolis extracts from stingless bees on antioxidant activity and total content of bioactive compounds. Food Research International, 230: 118544, doi: 10.1016/j.foodres.2026.118544.
24. Gültekin, D. C., Turan, İ., Akmeşe, O., Gültekin, E., Demir, S., Aliyazıcıoğlu, Y. (2026). LC–MS/MS phenolic profiling and antioxidant, antimicrobial, and antidiabetic activities of Caucasian propolis. Chemistry & Biodiversity, 23(3): e03377, doi: 10.1002/cbdv.202503377.
25. Iriondo-Dehond, A., Rodríguez Casas, A., Del Castillo, M. D. (2021). Interest of coffee melanoidins as sustainable healthier food ingredients. Frontiers in Nutrition, 8: 730343, doi: 10.3389/fnut.2021.730343.
26. Jeszka-Skowron, M., Sentkowska, A., Pyrzyńska, K., De Peña, M. P. (2016). Chlorogenic acids, caffeine content and antioxidant properties of green coffee extracts: Influence of green coffee bean preparation. European Food Research and Technology, 242(8): 1403–1409, doi: 10.1007/s00217-016-2643-y.
27. Kocakaplan, Z. B., Ozkan, G., Kamiloglu, S., Capanoglu, E. (2024). Valorization of pineapple (Ananas comosus) by-products in milk coffee beverage: Influence on bioaccessibility of phenolic compounds. Plant Foods for Human Nutrition, 79(2): 300–307, doi: 10.1007/s11130-024-01159-4.
28. Kocer, S., Copur, O. U., Tamer, C. E., Suna, S., Kayahan, S., Akman, O. (2024). Optimization and characterization of chestnut shell pigment extract obtained by microwave-assisted extraction using response surface methodology. Food Chemistry, 443: 138424, doi: 10.1016/j.foodchem.2024.138424.
29. Kolayli, S., Yaylaci Karahalil, F., Celebi, Z. B., Boztaş, G. D., Capanoglu, E. (2025). Artificial neural networks (ANN) modeling for ethanolic propolis extracts. Food Biophysics, 20(3): 117–129, doi: 10.1007/s11483-025-09995-2.
30. Konuk Takma, D., Ulkeryıldız Balcık, E., Baysan, U., Zungur Bastıoğlu, A., Coşkun, N. O., Şahin Nadeem, H., Koc, M. (2022). Encapsulation and in vitro evaluation of phenolic compounds of propolis by spray and freeze drying. Journal of Food Processing and Preservation, 46(11): e17047, doi: 10.1111/jfpp.17047.
31. Kumaran, A., Joel, R. (2006). Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chemistry, 97(1): 109–114, doi: 10.1016/j.foodchem.2005.03.032.
32. Kurek-Górecka, A., Kara, Y., Pokajewicz, K., Ben Hammouda, I., Wieczorek, P. P., Marciniak, D., Balwierz, R., Kłósek, M., Czuba, Z. P., Kolaylı, S. (2025). Phenolic content, volatile compounds and antioxidant activity in pooled propolis samples from Turkey and Poland. European Food Research and Technology, 251(8): 2199–2210, doi: 10.1007/s00217-025-04756-4.
33. Lucas-González, R., Viuda-Martos, M., Álvarez, J. A. P., Fernández-López, J. (2018). Changes in bioaccessibility, polyphenol profile and antioxidant potential of flours obtained from persimmon fruit (Diospyros kaki) co-products during in vitro gastrointestinal digestion. Food Chemistry, 256: 252–258, doi: 10.1016/j.foodchem.2018.02.128.
34. Murthy, P. S., Naidu, M. M. (2012). Recovery of phenolic antioxidants and functional compounds from coffee industry by-products. Food and Bioprocess Technology, 5(3): 897–903, doi: 10.1007/s11947-010-0363-z.
35. Mutlu, C., Demir, Z., Ozkan, A., Erbas, M. (2024). Phenolic changes in propolis during in vitro digestion and cytotoxic effects on human cancer cell lines. British Food Journal, 126(11): 3906–3921, doi: 10.1108/BFJ-09-2023-0846.
36. Minekus, M., Alminger, M., Alvito, P., Ballance, S., Bohn, T., Bourlieu, C., Brodkorb, A., et al. (2014). A standardised static in vitro digestion method suitable for food – an international consensus. Food & Function, 5(6): 1113–1124, doi: 10.1039/C3FO60702J.
37. Okuyan, S., Mehmetoğlu, S., Çakıcı, N. (2020). Antioxidant variability of propolis collected from different zones in hives. Bee Studies, 12(1): 1–4, doi: 10.51458/BSTD.2021.1.
38. Ozdal, T., Ceylan, F. D., Eroğlu, N., Kaplan, M., Oktem Olgun, E., Çapanoğlu, E. (2019). Investigation of antioxidant capacity, bioaccessibility and LC-MS/MS phenolic profile of Turkish propolis. Food Research International, 122: 528–536, doi: 10.1016/j.foodres.2019.05.028.
39. Ozkan, G., Sanli, I., Mottola, S., De Marco, I., Capanoglu, E. (2025). Supercritical antisolvent processing of propolis extract: Solubility and release behaviour of bioactive compounds in different food simulants. Food Chemistry, 489: 144742, doi: 10.1016/j.foodchem.2025.144742.
40. Özkök, A., Keskin, M., Tanuğur Samancı, A. E., Yorulmaz Önder, E., Takma, Ç. (2021). Determination of antioxidant activity and phenolic compounds for basic standardization of Turkish propolis. Applied Biological Chemistry, 64: 37, doi: 10.1186/s13765-021-00608-3.
41. Özkök, D., Silici, S. (2017). Antioxidant activities of honeybee products and their mixtures. Food Science and Biotechnology, 26(1): 201–206, doi: 10.1007/s10068-017-0027-0.
42. Paula, V. B., Estevinho, L. M., Cardoso, S. M., Dias, L. G. (2023). Comparative methods to evaluate the antioxidant capacity of propolis: An attempt to explain the differences. Molecules, 28(12): 4847, doi: 10.3390/molecules28124847.
43. Popova, M., Trusheva, B., Bankova, V. (2022). Chemistry and applications of propolis. H. N. Murthy (Ed.), Gums, Resins and Latexes of Plant Origin (pp. 1–33). Cham, Switzerland: Springer, doi: 10.1007/978-3-030-76523-1_38-1.
44. Sarapa, A., Peter, A., Buettner, A., et al. (2025). Organoleptic and chemical properties of propolis: A review. European Food Research and Technology, 251: 1331–1352, doi: 10.1007/s00217-025-04708-y.
45. Saroglu, O., Karadag, A. (2024). Propolis-loaded liposomes: Characterization and evaluation of the in vitro bioaccessibility of phenolic compounds. ADMET and DMPK, 12(1): 209–224, doi: 10.5599/admet.2204.
46. Saura-Calixto, F., Serrano, J., Goñi, I. (2007). Intake and bioaccessibility of total polyphenols in a whole diet. Food Chemistry, 101(2): 492–501, doi: 10.1016/j.foodchem.2006.02.006.
47. Sinković, S., Pehnec, M., Žarković, I. (2023). Influence of encapsulation of propolis extract with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) on polyphenolic contents during in vitro simulation of digestion. Applied Sciences, 13(16): 9357, doi: 10.3390/app13169357.
48. Siripatrawan, U., Vitchayakitti, W., Sanguandeekul, R. (2012). Antioxidant and antimicrobial properties of Thai propolis extracted using ethanol aqueous solution. International Journal of Food Science & Technology, 47(1): 22–27, doi: 10.1111/j.1365-2621.2011.02804.x.
49. Stavropoulou, M. I., Termentzi, A., Kasiotis, K. M., Cheilari, A., Stathopoulou, K., Machera, K., Aligiannis, N. (2021). Untargeted ultrahigh-performance liquid chromatography-hybrid quadrupole-orbitrap mass spectrometry (UHPLC-HRMS) metabolomics reveals propolis markers of Greek and Chinese origin. Molecules, 26(2): 456, doi: 10.3390/molecules26020456.
50. Sukri, N., Putri, T. T. M., Mahani, Nurhadi, B. (2023). Characteristics of propolis encapsulated with gelatin and sodium alginate by complex coacervation method. International Journal of Food Properties, 26(1): 696–707, doi: 10.1080/10942912.2023.2179635
51. Šuran, J., Cepanec, I., Mašek, T., Radić, B., Radić, S., Tlak Gajger, I., Vlainić, J. (2021). Propolis extract and its bioactive compounds—From traditional to modern extraction technologies. Molecules, 26(10): 2930. doi: 10.3390/molecules26102930.
52. Suna, S., Kalkan, S., Dinç, M., Utku, Ö. (2023). Production of low calorie persimmon marmalades with stevia and maltitol: Physicochemical properties and in vitro bioaccessibility of polyphenols. Journal of Food Measurement and Characterization, 17(1): 1082–1095, doi: 10.1007/s11694-022-01676-9.
53. Tagliazucchi, D., Verzelloni, E., Bertolini, D., Conte, A. (2010). In vitro bio-accessibility and antioxidant activity of grape polyphenols. Food Chemistry, 120(2): 599–606, doi: 10.1016/j.foodchem.2009.10.030.
54. Tomšič, R., Heath, D., Heath, E., Markelj, J., Kandolf Borovšak, A., Prosen, H. (2020). Determination of neonicotinoid pesticides in propolis with liquid chromatography coupled to tandem mass spectrometry. Molecules, 25(24): 5870, doi: 10.3390/molecules25245870.
55. Toreti, V. C., Sato, H. H., Pastore, G. M., Park, Y. K. (2013). Recent progress of propolis for its biological and chemical compositions and its botanical origin. Evidence-Based Complementary and Alternative Medicine, 2013: 697390, doi: 10.1155/2013/697390.
56. Tumbarski, Y., Todorova, M., Topuzova, M. G., Petkova, N. (2023). Comparative study on physicochemical, antioxidant and antimicrobial properties of propolis collected from different regions of Bulgaria. International Journal of Molecular Sciences, 24: 14336, doi: 10.3390/ijms241814336.
57. Ulloa, P. A., Vidal, J., Ávila, M. I., Labbe, M., Cohen, S., Salazar, F. N. (2017). Effect of the addition of propolis extract on bioactive compounds and antioxidant activity of craft beer. Journal of Chemistry, 6716053, doi: 10.1155/2017/6716053.
58. Vieira, A. L. S., Correia, V. T. V., Ramos, A. L. C. C., da Silva, N. H. A., Jaymes, L. A. C., Melo, J. O. F., de Paula, A. C. C. F. F., Garcia, M. A. V. T., Araújo, R. L. B. (2023). Evaluation of the chemical profile and antioxidant capacity of green, brown, and dark propolis. Plants, 12(18): 3204, doi: 10.3390/plants12183204.
59. Wu, H., Barrow, C., Dunshea, F. R., Suleria, H. A. (2023). Phenolic bioaccessibility, antioxidant, and antidiabetic effects of indigenous fermented coffee beans after simulated gastrointestinal digestion and colonic fermentation. Food Bioscience, 54: 102920, doi: 10.1016/j.fbio.2023.102920.
60. Yesiltas, B., Capanoglu, E., Firatligil-Durmus, E., Sunay, A. E., Samanci, T., Boyacioglu, D. (2014). Investigating the in-vitro bioaccessibility of propolis and pollen using a simulated gastrointestinal digestion system. Journal of Apicultural Research, 53(1): 101–108, doi: 10.3896/IBRA.1.53.1.10.
61. Zulhendri, F., Lesmana, R., Tandean, S., Christoper, A., Chandrasekaran, K., Irsyam, I., Suwantika, A. A., Abdulah, R., Wathoni, N. (2022). Recent update on the anti-inflammatory activities of propolis. Molecules, 27: 8473, doi: 10.3390/molecules27238473.