KEÇİBOYNUZU EKSTRAKTINDA BULUNAN D-PİNİTOLÜN ÇOK AŞAMALI ZENGİNLEŞTİRME PROSESİ İLE KONSANTRASYONU

Öz

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Anahtar Kelimeler

• Keçiboynuzu, D-pinitol, zenginlefltirme, fermantasyon, ultrafiltrasyon

CONCENTRATION OF D-PINITOL in CAROB EXTRACT by USING MULTI-STAGE ENRICHMENT PROCESSES

Öz

D-pinitol, a cyclic sugar alcohol, is claimed to be a potential therapeutic compound related to theillnesses arising from insulin mechanism. Carob is a rich source of this compound and has recentlybegun to be used in different separation and purification studies for obtaining D-pinitol. In this study,different enrichment processes were applied to concentrate the D-pinitol content of the carob extract.To determine the effectiveness of the processes applied for concentration of the target compound incarob extract, removal of other impurities (mainly sugars) was used as an important indicator. Accordingto the results; the highest increase in D-pinitol concentration was observed in the enrichment processcombining the techniques such as ethanol fermentation, membrane filtration and solvent extraction. Atthe end of this multi-stage process, D-pinitol concentration increased approximately four-fold (37.53g/100 mL dry weight) when compared to its initial level (9.38 g/100 mL dry weight) in carob extract

Anahtar Kelimeler

• Carob, D-pinitol, enrichment, fermentation, ultrafiltration

Kaynakça

1. Batlle, I, Tous, J. 1997. Promoting the conservation and use of underutilized and neglected crops. 17. Carob tree: Ceratonia siliqua L. 17. Institute of Plant Genetics and Crop Plant Research, Gatersleben/International Plant Genetic Resources Institute, Rome, Italy.
2. Bravo, L, Saura-Calixto, F. 1994. Composition and potential uses of mesquite pods (Prosopis pallida L): comparison with carob pods (Ceratonia siliqua L). J. Sci. Food Agric., 65, 303-306.
3. Avallone, R, Plessi, M, Baraldi, M, Monzani, A. 1997. Determination of chemical composition of carob (Ceratonia siliqua): Protein, fat, carbohydrates, and tannins. J. Food Compos. Anal., 10, 166-172.
4. Biner, B, Gubbuk, H, Karhan, M, Aksu, M, Pekmezci, M. 2007. Sugar profiles of the pods of cultivated and wild types of carob bean (Ceratonia siliqua L.) in Turkey. Food Chem., 100, 1453-1455. 5. Petit, MD, Pinilla, JM. 1995. Production and purification of a sugar syrup from carob pods. LWT - Food Science and Technology, 28, 145-152.
5. Baumgartner, S, Genner-Ritzmann, R, Haas, J, Amado, R, Neukom, H. 1986. Isolation and identification of cyclitols in carob pods (Ceratonia siliqua L.). J. Agric. Food. Chem., 34, 827-829.
6. Tetik, N, Turhan, I, Oziyci, HR, Karhan, M. 2011. Determination of D-pinitol in carob syrup. Int. J. Food Sci. Nutr., 62, 572-576.
7. Poongothai, G, Sripathi, SK. 2013. A review on insulinomimetric pinitol from plants. International Journal of Pharma and Bio Sciences, 4, 992-1009. 9. Ostlund, RE, Sherman, WR. October 1998. Pinitol and derivatives thereof for the treatment of metabolic disorders, US Patent US5827896 A.
8. Kim, JI, Kim, J, Kang, MJ, Lee, MS, Kim, JJ, Cha, IJ. 2004. Effects of pinitol isolated from soybeans on glycaemic control and cardiovascular risk factors in Korean patients with type II diabetes mellitus: a randomized controlled study. Eur. J. Clin. Nutr., 59, 456-458.

9. Geethan, P, Prince, P. 2008. Antihyperlipidemic effect of D-pinitol on streptozotocin-induced diabetic wistar rats. J. Biochem. Mol. Toxicol., 22, 220-224.
10. Lin, TH, Tan, TW, Tsai, TH, Chen, CC, Hsieh, TF, Lee, SS, Liu, HH, Chen, WC, Tang, CH. 2013. D-pinitol inhibits prostate cancer metastasis through inhibition of αVβ3 integrin by modulating FAK, c-Src and NF-κB pathways. Int. J. of Mol. Sci., 14, 9790-9802.
11. Nestler, JE, Jakubowicz, DJ, Reamer, P, Gunn, RD, Allan, G. 1999. Ovulatory and metabolic effects of D-chiro-inositol in the polycystic ovary syndrome. N. Engl. J. Med., 340, 1314-1320.
12. Lin, X, Ma, L, Gopalan, C, Ostlund, RE. 2009. D-chiro-Inositol is absorbed but not synthesised in rodents. Br. J. Nutr., 102, 1426-1434.
13. Streeter, JG. 2001. Simple partial purification of D-pinitol from soybean leaves. Crop Sci., 41, 1985-1987.
14. Shin, YC, Jeon, YJ, Kim, JJ, Choi, CM. October 2003. Method of recovering pinitol or chiro-inositol in high yield from soy fractions, US Patent US20030186401 A1.
15. Camero, BM, Merino, CS. March 2004. Method of obtaining pinitol from carob extracts. US Patent US6699511 B2.
16. Rabinowitz, I. March 1992. Chromatographic method of purifying cyclitols, US Patent US5096594 A.
17. Turhan, I, Bialka, KL, Demirci, A, Karhan, M. 2010. Ethanol production from carob extract by using Saccharomyces cerevisiae. Bioresour. Technol., 101, 5290-5296.
18. Wisselink, HW, Toirkens, MJ, Wu, Q, Pronk, JT, Van Maris, AJ. 2009. Novel evolutionary engineering approach for accelerated utilization of glucose, xylose, and arabinose mixtures by engineered Saccharomyces cerevisiae strains. Appl. Environ. Microbiol., 75, 907-914.
19. Hahn-Hägerdal, B, Karhumaa, K, Jeppsson, M, Gorwa-Grauslund, MF. 2007. Metabolic engineering for pentose utilization in Saccharomyces cerevisiae, Advances in Biochemical Engineering/Biotechnology: Biofuels, 108, 147-177.
20. Cemeroğlu, B. 2007. Gıda Analizlerinde Genel Yöntemler, Gıda Analizleri, Gıda Teknolojisi Derneği Yayınları No:34, Ankara, 535 p.
21. Yoon, SH, Mukerjea, R, Robyt, JF. 2003. Specificity of yeast (Saccharomyces cerevisiae) in removing carbohydrates by fermentation. Carbohydr. Res., 338, 1127-1132.
22. Agrawal, P, Rabinowitz, I. May 1997. Method of purifying cyclitols, US Patent US5626847 A.
23. Roukas, T. 1993. Ethanol production from carob pods by Saccharomyces cerevisiae. Food Biotechnol., 7 (1993) 159-176.