ULTRASOUND-ASSISTED EXTRACTION OF PEANUT (Arachis Hypogaea L.) OIL UNDER VARYING POWER AND FREQUENCIES USING DIFFERENT SOLVENTS

Öz

The effects of extraction frequency and ultrasonic power on the extraction yield and fatty acid composition of peanut oil were studied. Also, the usability of ethanol as a less toxic and environmentally friendly alternative to hexane –the most common solvent in conventional oil extraction– was investigated. The highest extraction yields for ethanol and hexane were respectively %40.55 and %45.19 at 37 kHz frequency and 80% ultrasonic power. It was determined that all samples extracted with hexane had higher extraction yields than those of ethanol. The differences between the ethanolic extraction yields at 37 and 80 kHz were statistically significant. The major factor affecting the extraction yield was the frequency for hexane; while interactional effects of all process conditions were significant for ethanolic extraction. The increment of ultrasonic frequency caused a decrease in the extraction yields. Results revealed that different processing conditions did not significantly affect fatty acid compositions. 

Anahtar Kelimeler

• Ultrasound-assisted extraction,Ethanol,Peanut oil,Oleic acid,Frequency,Ultrasonic power

YER FISTIĞI (Arachis Hypogaea L.) YAĞININ FARKLI ÇÖZÜCÜLER KULLANILARAK DEĞİŞKEN GÜÇ VE FREKANSLARDA ULTRASON DESTEKLİ EKSTRAKSİYONU

Öz

Bu çalışmada, ultrason destekli ekstraksiyonla elde edilen yer fıstığı yağının ekstrakiyon verimi ve yağ asidi bileşimi üzerine farklı frekans ve ultrasonik gücün etkileri incelenmiştir. Ayrıca yağ ekstraksiyonunda yaygın olarak tercih edilen hekzan yerine, daha az toksik ve çevreye daha duyarlı bir çözücü olan etanolün kullanılabilirliği araştırılmıştır. Etanol ve hekzana ait en yüksek ekstraksiyon verimleri 37 kHz frekans ve %80 güçte elde edilmiştir ve sırasıyla %40.55 ve %45.19 olarak hesaplanmıştır. Hekzan verimlerinin tüm örneklerde etanole göre yüksek olduğu tespit edilmiştir. 37 kHz ve 80 kHz frekanstaki etanol ekstraktlarının verimleri arasındaki fark istatistik olarak önemli bulunmuştur. Varyans analizi sonucunda, hekzan verimleri üzerine yalnızca frekansın etkili olduğu belirlenirken, etanol verimleri üzerine işlem koşullarının interaksiyon etkileri tespit edilmiştir. Bulgular frekans artışının verimde azalmaya neden olduğunu ortaya koymaktadır. Ayrıca, uygulanan farklı işlem koşullarının yağ asidi bileşiminde önemli bir etki göstermediği tespit edilmiştir.

Anahtar Kelimeler

• Ultrason destekli ekstraksiyon,Etanol,Yer fıstığı yağı,Oleik asit,Frekans,Ultrasonik güç

Kaynakça

1. Afroz Bakht, M., Geesi, M.H., Riadi, Y., Imran, M., Imtiyaz Ali, M., Ahsan, M.J., & Ajmal, N. (2019). Ultrasound-assisted extraction of some branded tea: Optimization based on polyphenol content, antioxidant potential and thermodynamic study. Saudi J Biol Sci 26(5): 1043-1052, doi:10.1016/j.sjbs.2018.07.013
2. Aluyor, E., Aluyor, P., Ozigagu, C. (2009). Effect of refining on the quality and composition of groundnut oil. Afr J Food Sci 3(8): 201-205.
3. Anonim (2012). Türk Gıda Kodeksi. Bitki Adı ile Anılan Yağlar Tebliği (2012/29). Gıda Tarım ve Hayvancılık Bakanlığı. 12 Nisan 2012 tarih ve 28262 sayılı Resmî Gazete, Ankara.
4. Baümler, E.R., Carrín, M.E., Carelli, A.A. (2016). Extraction of sunflower oil using ethanol as solvent. J Food Eng 178: 190-197, doi:10.1016/j.jfoodeng.2016.01.020
5. Baümler, E.R., Carrín, M.E., Carelli, A.A. (2017). Diffusion of tocopherols, phospholipids and sugars during oil extraction from sunflower collets using ethanol as solvent. J Food Eng 194: 1-8, doi:10.1016/j.jfoodeng.2016.09.003
6. Breil, C., Meullemiestre, A., Vian, M., Chemat, F. (2016). Bio-Based Solvents for Green Extraction of Lipids from Oleaginous Yeast Biomass for Sustainable Aviation Biofuel. Molecules 21(2): 196, doi:10.3390/molecules21020196
7. Chanioti, S., Tzia, C. (2017). Optimization of ultrasound-assisted extraction of oil from olive pomace using response surface technology: Oil recovery, unsaponifiable matter, total phenol content and antioxidant activity. LWT - Food Sci Technol 79: 178-189, doi:10.1016/j.lwt.2017.01.029
8. Da Porto, C., Porretto, E., Decorti, D. (2013). Comparison of ultrasound-assisted extraction with conventional extraction methods of oil and polyphenols from grape (Vitis vinifera L.) seeds. Ultrason Sonochem 20(4): 1076-1080, doi:10.1016/j.ultsonch.2012.12.002

9. Dong, J., Liu, Y., Liang, Z., Wang, W. (2010). Investigation on ultrasound-assisted extraction of salvianolic acid B from Salvia miltiorrhiza root. Ultrason Sonochem 17(1): 61-65, doi:10.1016/j.ultsonch.2009.05.006
10. Escorsim, A.M., da Rocha, G., Vargas, J.V.C., Mariano, A.B., Ramos, L.P., Corazza, M.L., Cordeiro, C.S. (2018). Extraction of Acutodesmus obliquus lipids using a mixture of ethanol and hexane as solvent. Biomass Bioenerg 108: 470-478, doi:10.1016/j.biombioe.2017.10.035
11. FAO (2019). FAOSTAT. Food and Agriculture Organization of the United Nations, Roma, İtalya.
12. Ferreira-Dias, S., Valente, D.G., Abreu, J.M.F. (2003). Comparison between ethanol and hexane for oil extraction from Quercus suber L. fruits. Grasas Aceites 54(4): 378-383, doi:10.3989/gya.2003.v54.i4.225
13. ISO (2011). Animal and vegetable fats and oils — Gas chromatography of fatty acid methyl esters — Part 2: Preparation of methyl esters of fatty acids. ISO 12966-2:2011. Geneva, Switzerland: ISO.
14. Kadiroğlu, A. (2018). Yerfıstığı Yetiştiriciliği. Batı Akdeniz Tarımsal Araştırma Enstitüsü Müdürlüğü. https://arastirma.tarimorman.gov.tr/batem/Belgeler/Kutuphane/Teknik%20Bilgiler/yerfistigi%20yetistiriciligi.pdf (Erişim tarihi: 8 Ekim 2019).
15. Li, H., Pordesimo, L., Weiss, J. (2004). High intensity ultrasound-assisted extraction of oil from soybeans. Food Res Int 37(7): 731-738, doi:10.1016/j.foodres.2004.02.016
16. Li, Y., Fine, F., Fabiano-Tixier, A.-S., Abert-Vian, M., Carre, P., Pages, X., Chemat, F. (2014). Evaluation of alternative solvents for improvement of oil extraction from rapeseeds. C R Chim 17(3): 242-251, doi:10.1016/j.crci.2013.09.002
17. Ma, Y., Wu, X., Zhao, L., Wang, Y., Liao, X. (2019). Comparison of the compounds and characteristics of pepper seed oil by pressure-assisted, ultrasound-assisted and conventional solvent extraction. Innov Food Sci Emerg 54: 78-86, doi:10.1016/j.ifset.2019.03.011
18. Mohammadpour, H., Sadrameli, S.M., Eslami, F., Asoodeh, A. (2019). Optimization of ultrasound-assisted extraction of Moringa peregrina oil with response surface methodology and comparison with Soxhlet method. Ind Crop Prod 131: 106-116, doi:10.1016/j.indcrop.2019.01.030
19. Muatasim, R., Ma, H., Yang, X. (2018). Effect of multimode ultrasound assisted extraction on the yield of crude polysaccharides from Lycium Barbarum (Goji). Food Sci Technol 38: 160-166, doi:10.1590/1678-457X.14417
20. Perrier, A., Delsart, C., Boussetta, N., Grimi, N., Citeau, M., Vorobiev, E. (2017). Effect of ultrasound and green solvents addition on the oil extraction efficiency from rapeseed flakes. Ultrason Sonochem 39: 58-65, doi:10.1016/j.ultsonch.2017.04.003
21. Rodriguez-Estrada, M.T., Paciulli, M., Cerretani, L., Chiavaro, E. (2017). Production of Vegetable Oils from Fruits, Oilseeds, and Beans: Conventional Processing and Industry Techniques. In: Edible Oils: Extraction, Processing, and Applications, Chemat, S. (Ed.), Boca Raton, FL: CRC Press, pp. 1-34
22. Sbihi, H.M., Nehdi, I.A., Mokbli, S., Romdhani-Younes, M., Al-Resayes, S.I. (2018). Hexane and ethanol extracted seed oils and leaf essential compositions from two castor plant (Ricinus communis L.) varieties. Ind Crop Prod 122: 174-181, doi:10.1016/j.indcrop.2018.05.072
23. Stevanato, N., da Silva, C. (2019). Radish seed oil: Ultrasound-assisted extraction using ethanol as solvent and assessment of its potential for ester production. Ind Crop Prod 132: 283-291, doi:10.1016/j.indcrop.2019.02.032
24. Taticchi, A., Selvaggini, R., Esposto, S., Sordini, B., Veneziani, G., Servili, M. (2019). Physicochemical characterization of virgin olive oil obtained using an ultrasound-assisted extraction at an industrial scale: Influence of olive maturity index and malaxation time. Food Chem 289: 7-15, doi:10.1016/j.foodchem.2019.03.041
25. Topdaş, E.F., Ertugay, M.F. (2013). Kayısıların Ultrases Yardımlı Ozmotik Kurutulması. GIDA, 38(5): 299-306, doi:10.5505/gida.2013.02886
26. Tüfekçi, S., Özkal, S.G. (2015). Application of Ultrasound in Food Drying. Pamukkale Univ Muh Bilim Derg 21(9): 408-413, doi:10.5505/pajes.2015.05902
27. Zhuang, X., Zhang, Z., Wang, Y., Li, Y. (2018). The effect of alternative solvents to n-hexane on the green extraction of Litsea cubeba kernel oils as new oil sources. Ind Crop Prod 126: 340-346, doi:10.1016/j.indcrop.2018.10.004