DETERMINATION OF THE LYOPHILIZED EXOPOLYSACCHARIDES (L-EPS) OF LACTOBACILLUS BACTERIA BIFIDOGENIC GROWTH STIMULATOR EFFECT

Öz

In this study, 119 bacteria belonging to the genus Lactobacillus were isolated from the gastrointestinal system of free-range village chickens obtained from different regions of Ankara. 11 isolates with high exopolysaccharide (EPS) production capacity were selected. Biochemical and molecular identification of the selected isolates was performed and according to the identification results, it was determined that 6 isolates were Lactobacillus salivarius, 2 isolates were Lactobacillus agilis, 2 isolates were Lactobacillus reuteri and 1 isolate was Lactobacillus saerimner. EPS of 3 strains producing high EPS (L. salivarius ZDM2132, BIS312, and BIS722) were isolated from the culture medium and lyophilized. In the study, the ability of Bifidobacterium gallinarum ATCC 33777 strain to ferment lyophilized EPS (l-EPS) obtained from L. salivarius ZDM2132, BIS312 and BIS722 bacteria were determined. In addition, the effects of l-EPS the bifidogenic growth stimulator (BGD) were compared to inulin that a commercial prebiotic. l-EPSs are fermented by B. gallinarum and have been shown to show a better BGD effect than inulin.

Anahtar Kelimeler

• Food industry,Exopolysaccharides,Prebiotic,Bifidogenic Growth Stimulator effect,Industrial biology

LACTOBACILLUS CİNSİ BAKTERİLERİN LİYOFİLİZE EKZOPOLİSAKKARİTLERİNİN BİFİDOBAKTERİLERİN GELİŞİMİNİ DÜZENLEYİCİ ETKİSİNİN BELİRLENMESİ

Öz

Bu çalışmada, Ankara ilinin farklı bölgelerinden temin edilen serbest dolaşan köy tavuklarının gastrointestinal sisteminden Lactobacillus cinsine ait 119 bakteri izole edilmiştir. Yüksek ekzopolisakkarit (EPS) üretim kapasitesine sahip 11 izolat seçilmiştir. Seçilen izolatların biyokimyasal ve moleküler tanımlamaları gerçekleştirilmiş ve tanımlama sonuçlarına göre 6 izolatın Lactobacillus salivarius, 2 izolatın Lactobacillus agilis, 2 izolatın Lactobacillus reuteri ve 1 izolatın da Lactobacillus saerimneri olduğu tespit edilmiştir. Yüksek EPS üreten 3 suşun (L. salivarius ZDM2132, BİS312 ve BİS722) EPS’leri kültür ortamından izole edilerek liyofilize edilmiştir. Bifidobacterium gallinarum ATCC 33777 suşunun, L. salivarius ZDM2132, BİS312 ve BİS722 bakterilerden elde edilen liyofilize EPS’yi (l-EPS) fermente edebilme kapasitesi ve bifidobakterilerin gelişimini düzenleyici (BGD) etkileri ticari bir prebiyotik olan inülin ile karşılaştırılmıştır. l-EPS’lerin B. gallinarum tarafından fermente edildiği ve inülinden daha iyi bir BGD etkisi gösterdiği gözlemlenmiştir.

Anahtar Kelimeler

• Biyoteknoloji,Endüstriyel biyoloji,Gıda endüstrisi,Prebiyotik,Ekzopolisakkarit,BGD etki,Lactobacillus salviravius

Kaynakça

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