ARBUTUS UNEDO L. YAPRAKLARINDAN FENOLİK BİLEŞİKLERİN EKSTRAKSİYONUNDA EKSTRAKSİYON KOŞULLARININ BELİRLENMESİ

Öz

Arbutus unedo yapraklarından biyoaktif bileşiklerin klasik çözücü ekstraksiyonundaki ekstraksiyon parametreleri tek faktörlü deney yaklaşımı kullanılarak incelenmiştir. Etanol (EtOH) konsantrasyonu (20-100 %), çözücü:katı oranı (10-90 ml/g), ekstraksiyon süresi (20-100 dak) ve ekstraksiyon sıcaklığının (15-70 °C) ekstraktların toplam fenolik içeriği (TPC), toplam flavonoid içeriği (TFC) ve toplam antioksidan aktivitesi (TAA) üzerindeki etkisi araştırılmıştır. Optimal koşullar çözücü konsantrasyonu 40 % etanol, çözücü:katı oranı 50:1, ekstraksiyon süresi 60 dakika ve ekstraksiyon sıcaklığı 55 °C olarak bulunmuştur. Bu koşullar altında TPC, TFC ve TAA değerleri sırasıyla 89.58 mg GAE/g dw, 28.62 mg CAT / g dw ve 85.25 % inh olarak elde edilmiştir. Ekstraktların özellikle flavonoid içeriğinin, etanol konsantrasyonu (r = 0.962), çözücü:katı oranı (r = 0.947), ekstraksiyon süresi (r = 0.793) ve sıcaklık (r = 0.935) gibi tüm ekstraksiyon koşulları altında antioksidan aktivitesi ile oldukça ilişkili olduğu bulunmuştur.

Anahtar Kelimeler

• Arbutus unedo yaprağı
• çözücü ekstraksiyonu
• polifenoller
• flavonoidler
• antioksidan aktivite

DETERMINATION OF THE EXTRACTION CONDITIONS OF PHENOLIC COMPOUNDS FROM ARBUTUS UNEDO L. LEAVES

Öz

Extraction parameters in classical solvent extraction of bioactive compounds from Arbutus unedo leaves were studied using a single factor experiment approach. Effects of ethanol (EtOH) concentration (20-100 %), solvent:solid ratio (10-90 ml/g), extraction time (20-100 min) and extraction temperature (15-70 °C) on total phenolic content (TPC), total flavonoid content (TFC) and total antioxidant activity (TAA) of the extracts were investigated. The optimal conditions were found as solvent concentration of 40 % EtOH, solvent:solid ratio of 50:1, extraction time of 60 min, and extraction temperature of 55 °C. Under these conditions, TPC, TFC and TAA were obtained as 89.58 mg GAE/g dw, 28.62 mg CAT/g dw and 85.25 % inh, respectively. It was found that especially the flavonoid content of the extracts was highly correlated with antioxidant activity under all extraction conditions such as ethanol concentration (r=0.962), solvent:solid ratio (r=0.947), extraction time (r=0.793) and temperature (r=0.935).

Anahtar Kelimeler

• Arbutus unedo leaves
• solvent extraction
• polyphenols
• flavonoids
• antioxidant activity

Kaynakça

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