Effect of Heat Treatment on Chlorophyll Degradation and Color Loss in Green Peas (in English)

Öz

Thermal degradation kinetics of chlorophyll and visual green color loss in green peas were investigated at 70°, 80°, 90° and 100 °C. The degradation of chlorophyll a and chlorophyll b followed a first-order kinetic model. The loss of visual green color, as represented by the change of the -a, the ratio -a/b and h (hue) values measured by tristimulus colorimeter, also followed a first-order reaction. The activation energies for chlorophylls a and b were determined as 47.78 and 26.77 kJ mol-1, while those for -a, -a/b and h values were found as 49.75, 56.04 and 55.06 kJ mol-1, respectively.

Anahtar Kelimeler

• Chlorophyll, kinetics, green peas, color

Bezelyelerde Klorofil Degradasyonu ve Renk Kaybı Üzerine Isıl İşlemin Etkisi (İngilizce)

Öz

Bezelyelerde klorofilin termal degradasyon kinetiği ve görünür yeşil renkteki kayıp 70°, 80°, 90° ve 100 °C’de araştırılmıştır. Klorofil a ve klorofil b’nin parçalanması birinci dereceden bir kinetik model izlemektedir. Tristimulus kolorimetresi ile ölçülen -a, -a/b ve h (hue) değerlerindeki değişim ile ortaya konulan görünür yeşil renkteki kayıp da birinci dereceden reaksiyona uymaktadır. Klorofil a ve b için aktivasyon enerjileri sırasıyla 47.78 and 26.77 kJ mol-1olarak belirlenirken; -a, -a/b ve h değerleri için aktivasyon enerjileri 49.75, 56.04 and 55.06 kJ mol-1 olarak saptanmıştır.

Anahtar Kelimeler

• Klorofil, kinetik, bezelye, renk

Kaynakça

1. Almela L, Fernández-López JA and Roca MJ. 2000. High-performance liquid chromatographic screening of chlorophyll derivatives produced during fruit storage. J. Chromatogr. A, 870: 483-489.
2. Steet JA and Tong CH. 1996. Degradation kinetics of green color and chlorophylls in peas by colorimetry and HPLC. J. Food Sci., 61: 924-927.
3. Tan CT and Francis FJ. 1962. Effect of processing temperature on pigments and color of spinach. J. Food Sci., 27: 232- 240. 4. Lajollo F, Tannenbaum SR. and Labuza TP. 1971. Reaction at limited water concentrations. Chlorophyll degradation. J. Food Sci., 36: 850-853.
4. Schwartz SJ and Von Elbe JH. 1983. Kinetics of chlorophyll degradation to pyropheophytin in vegetables. J. Food Sci., 48: 1303-1306.
5. Canjura FL, Schwartz SJ and Nunes RV. 1991. Degradation kinetics of chlorophylls and chlorophyllides. J Food Sci., 56: 1639-1643. 7. Schwartz SJ and Lorenzo TV. 1991. Chlorophyll stability during continuous aseptic processing and storage. J. Food Sci., 56: 1059-1062.
6. Tonucci LH and Von Elbe JH. 1992. Kinetics of the formation of zinc complexes of chlorophyll derivatives. J. Agric. Fo- od Chem., 40: 2341-2344.
7. Canjura FL and Schwartz SJ. 1991. Separation of chlorophyll compounds and their polar derivatives by high-perfor- mance liquid chromatography. J. Agric. Food Chem., 39: 1102-1105.
8. Heaton JW and Marangoni AG. 1996. Chlorophyll degradation in processed foods and senescent plant tissues. Trends Food Sci. Technol., 7: 8-15.

9. Minguez-Mosquera MI, Garrido-Fernandez J and Gandul-Rojas B. 1989. Pigment changes in olives during fermentati- on and brine storage. J. Agric. Food Chem., 37: 8-11.
10. Mangos TJ and Berger RG. 1997. Determination of major chlorophyll degradation products. Z. Lebensm.Unters. Forsch. A, 204: 345-350.
11. Van Boekel MAJS. 1999. Testing of kinetic models: usefulness of the multiresponse approach as applied to chlorophyll degradation in foods. Food Res. Int., 32: 261-269.
12. Van Boekel MAJS. 2000. Kinetic modelling in food science: a case study on chlorophyll degradation in olives. J. Sci. Food Agric., 80: 3-9.
13. La Borde LF and Von Elbe JH. 1990. Zinc complex formation in heated vegetable purees. J. Agric. Food Chem., 38: 484-487.
14. Weemaes C, Ooms V, Van Loey AM and Hendrickx ME. 1999. Kinetics of chlorophyll degradation and color loss in hea- ted broccoli juice. J. Agric. Food Chem., 47: 2404-2409.
15. White RC, Jones ID and Gibbs E.1963. Determination of chlorophylls, chlorophylldes,pheophytins, and pheophorbides in plant material. J. Food Sci., 28: 431- 436.
16. Von Elbe JH and Schwartz SJ. 1996. Colorants. In: Food Chemistry (Ed. O.R. Fennema), Marcel Dekker, New York, pp.651-722,
17. Clydesdale FM and Francis FJ. 1968. Chlorophyll changes in thermally processed spinach as influenced by enzyme conversion and pH adjustment. Food Technol., 22: 793-796.
18. Buckle KA and Edwards RA. 1970. Chlorophyll color and pH changes in HTST processed green pea puree. J. Food Technol., 5: 173-178.
19. Gnanasekharan V, Shewfelt RL and Chinnan MS. 1992. Detection of color changes in green vegetables. J Food Sci., 57: 149-154.
20. Barrett DM, Garcia EL, Russell GF, Ramirez E and Shirazi A. 2000. Blanch time and cultivar effects on quality of fro- zen and stored corn and broccoli. J. Food Sci., 65: 534-540.
21. Gunawan, MI and Barringer SA. 2000. Green color degradation of blanched broccoli (Brassica oleracea) due to acid and microbial growth. J. Food Process. Pres., 24: 253-263.
22. Tijkens LMM, Barringer SA and Biekman ESA. 2001. Modelling the effect of pH on the colour degradation of blanched broccoli. Innov Food Sci Emerg Technol., 2:315-322.
23. Cano, MP and Marin MA 1992. Pigment composition and color of frozen and canned kiwi fruit slices. J. Agric. Food Chem., 40: 2141-2146.
24. Labuza, TP and Riboh D. 1982. Theory and application of Arrhenius kinetics to the prediction of nutrients losses in fo- ods. Food Technol., 36: 66-74.
25. Labuza, TP. 1984. Application of chemical kinetics to deterioration of foods. J. Chem. Educ., 61: 348-358.
26. Hayakawa KI and Timbers GE. 1977. Influence of heat treatment on the quality of vegetables: changes in visual gre- en color. J Food Sci., 42: 778-781.
27. Schwartz SJ, Woo SL and Von Elbe JH. 1981. High performance liquid chromatography of chlorophylls and their deri- vatives in fresh and processed spinach. J. Agric. Food Chem., 29: 533-535.
28. Gupte SM and Francis FJ. 1964. Effect of pH adjustment high-temperature short-time processing on color and pigment retention in spinach puree. Food Technol., 18: 1645-1658.