Chitosan in the Fruit and Vegetable Processing Industry (Turkish with English Abstract)

Öz

Chitosan is a modified and natural carbohydrate polymer which is obtained from the partial deacetylation of chitin, and includes the copolymer of glucosamine and N-acetyl glucosamine. Chitosan, which is a microbial polysaccharide, is used as an antimicrobial agent for its bacteriocidal and fungicidal functions. In addition to these properties, it is also used for edible film-forming, reducing the acidity of fruit juices, fining agent, browning inhibitor, and color preservative in the fruit and vegetable industry. Chitosan as a biopolymer can be obtained from many natural sources, has a wide application area in the fruit and vegetable industry due to its non-toxic properties involving living organisms, its biodegradability, biocompatibility, chemical, and physical properties because it shows superior features compared to the other biopolymers. In this review, the use of chitosan, which has not been used as a widespread application in the fruit and vegetable processing industry, will be summarized.

Anahtar Kelimeler

• Chitosan, microbial polysaccharide, fruit and vegetable processing industry

Meyve-Sebze İşleme Endüstrisinde Kitosan Kullanımı

Öz

Kitosan, kitinin kısmi deasetilasyonuyla elde edilen, glukozamin ve N-asetil glukozamin kopolimerini içeren, modifiye ve doğal bir karbonhidrat polimeridir. Mikrobiyel bir polisakkarit olan kitosan, meyve-sebze endüstrisinde, bakteriyosidal ve fungisidal özellik göstererek antimikrobiyel madde görevi görmesinin yanı sıra, yenilebilir film oluşturma, meyve sularının asitliğini azaltma, durultma yardımcı maddesi, esmerleşmeyi önleyici ve renk koruyucu olarak da kullanılmaktadır. Doğada bulunan kaynaklardan elde edilebilen bir biyopolimer olan kitosan, canlılara karşı toksik özelliğinin olmaması, biyolojik olarak parçalanabilirliği, biyouyumluluğu, kimyasal ve fiziksel özellikleri bakımından diğer biyopolimerlere göre üstün özellikler göstermesi nedeniyle meyve-sebze endüstrisi için geniş kullanım alanına sahiptir. Bu derlemede, gıda sanayi için henüz yaygın kullanımı bulunmayan kitosanın meyve-sebze işleme endüstrisindeki kullanım alanları özetlenmiştir.

Anahtar Kelimeler

• Kitosan, mikrobiyel polisakkarit, meyve-sebze işleme endüstrisi

Kaynakça

1. Yazgan ‹. 2010. Kitosanın kimyasal modifikasyonu. Ege Üniversitesi Fen Bilimleri Enstitüsü Biyoteknoloji Anabilim Dalı Yüksek Lisans Tezi, ‹zmir, Türkiye, 98 s.
2. Cemero¤lu B (ed). 2009. Meyve ve Sebze ‹flleme Teknolojisi. 1. Cilt. 3. Baskı. Gıda Teknolojisi Derne¤i Yayınları No:38, Bizim Grup Basımevi Ankara, Türkiye, 707p.
3. Koç B, Özkan M. 2011. Gıda Endüstrisinde Kitosanın Kullanımı. GIDA, 36 (3): 161-168.
4. Demir A, Seventekin N. 2009. Kitin, Kitosan ve Genel Kullanım Alanları. Teknolojik Arafltırmalar: TTED, 3(2): 92-103.
5. Gökçe Y. 2008. Kitosan Nanoparçacıklarının Sentezi. Ankara Üniversitesi Fen Bilimleri Enstitüsü Kimya Mühendisli¤i Anabilim Dalı Yüksek Lisans Tezi, Ankara, Türkiye, s. 3-16.
6. Tarım E. 2011. Kitosan ile sulu çözeltilerden krom uzaklafltırılması. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Kimya Anabilim Dalı Yüksek Lisans Tezi, Isparta, Türkiye, s. 14-16.
7. Uslu B, Arbak S. 2010. Doku Mühendisli¤inde Kitozanın Kullanım Alanları. ACU Sa¤lık Bil Derg, 1(3): 128-135.
8. Rinaudo M. 2006. Chitin and chitosan: Properties and applications. Prog Polym Sci, 31: 603-632.

9. Prashanth KVH, Tharanathan RN. 2007. Chitin/ chitosan: modifications and their unlimited application potential-an overview. Trends Food Sci Technol, 18: 117-131.
10. Shaidi F, Arachchi JKV, Jeon YJ. 1999. Food applications of chitin and chitosans. Trends Food Sci Technol, 10: 37-51.
11. Devlieghere F, Vermeulen A, Debevere J. 2004. Chitosan: antimicrobial activity, interactions with food components and applicability as a coating on fruit and vegetables. Food Microbiol, 21: 703-714.
12. Bostan K, Aldemir T, Aydın A. 2007. Kitosan ve antimikrobiyel aktivitesi. Türk Mikrobiyol Cem Derg, 37(2): 118-127.
13. Moreira MR, Roura SI, Ponce A. 2011. Effectiveness of chitosan edible coatings to improve microbiological and sensory quality of fresh cut broccoli. LWT Food Sci Technol, 44: 2335-2341.
14. Dutta PK, Tripathi S, Mehrotra GK, Dutta J. 2009. Perspectives for chitosan based antimicrobial films in food applications. Food Chem, 114: 1173-1182.
15. Aranaz I, Mengibar M, Harris R, Panos I, Miralles B, Acosta N, Galed G, Heras A. 2009. Functional Characterization of Chitin and Chitosan. Curr Chem Biol., 3: 203-230.
16. Xia W, Liu P, Zhang J, Chen J. 2011. Biological activities of chitosan and chitooligosaccharides. Food Hydrocoll, 25: 170-179.
17. Chung YC, Chen CY. 2008. Antibacterial characteristics and activity of acid-soluble chitosan. Bioresource Technol, 99: 2806-2814.
18. Zhang H, Li R, Liu W. 2011. Effects of Chitin and Its Derivative Chitosan on Postharvest Decay of Fruits: A Review, Int. J. Mol. Sci., 12: 917-934.
19. Yang LY, Zhang JL, Bassett CL, Meng XH. 2012. Difference between chitosan and oligochitosan in growth of Monilinia fructicola and control of brown rot in peach fruit. LWT Food Sci Technol, 46: 254-259.
20. Meng X, Yang L, Kennedy JF, Tian S. 2010. Effects of chitosan and oligochitosan on growth of two fungal pathogens and physiological properties in pear fruit. Carbohyd Polym, 81: 70-75.
21. Lou MM, Zhu B, Muhammad ‹, Li B, Xie GL, Wang YL, Li HY, Sun GC. 2011. Antibacterial activity and mechanism of action of chitosan solutions against apricot fruit rot pathogen Burkholderia seminalis. Carbohyd Res, 34: 1294-1301.
22. Munoz Z, Moret A, Garces S. 2009. Assessment of chitosan for inhibition of Colletotrichum sp. on tomatoes and grapes. Crop Protection, 28: 36-40. 27. Badawy MEI, Rabea EI. 2009. Potential of the biopolymer chitosan with different molecular weights to control postharvest gray mold of tomato fruit. Postharvest Biol Technol, 51: 110-117.
23. Martin-Diana AB, Rico D, Barat JM, Barry- Ryan C. 2009. Orange juices enriched with chitosan: Optimisation for extending the shelf-life. Innov Food Sci Emerg Technol, 10: 590–600.
24. Knatt SR, Chander R, Sharma A. 2008. Chitosan glucose complex – A novel food preservative. Food Chem., 106: 521–528.
25. Ayana B, Turhan KN. 2010. Gıda Ambalajlamasında Antimikrobiyel Madde ‹çeren Yenilebilir Filmler/ Kaplamalar ve Uygulamaları. GIDA, 35(2), 151-158.
26. Aider M. 2010. Chitosan application for active bio-based films production and potential in the food industry: Review. LWT Food Sci Technol, 43: 837-842.
27. Kurt fi, Zorba Ö. 2005. Kitin, Kitosan ve Türevlerinin Gıdalarda Kullanım Olanakları. GIDA, 30(6): 371-378.
28. Sim ADN, Tudela JA, Allende A, Puschmann R, Gil MI. 2009. Edible coatings containing chitosan and moderate modified atmospheres maintain quality and enhance phytochemicals of carrot sticks. Postharvest Biol Technol, 51: 364-370.
29. Krasaekoopt W, Mabumrung J. 2008. Microbiological evaluation of edible coated freshcut cantaloupe. Nat Sci, 42: 552–557.
30. Vu KD, Hollingsworth RG, Leroux E, Salmieri S, Lacroix M. 2011. Development of edible bioactive coating based on modified chitosan for increasing the shelf life of strawberries. Food Res Int, 44: 198-203.
31. Chien P, Sheu F, Yang F. 2007. Effects of edible chitosan coating on quality and shelf life of sliced mango fruit. J Food Eng, 78: 225-229.
32. Sánchez-González L, Pastor C, Vargas M, Chiralt A, González-Mart nez C, Cháfer M. 2011. Effect of hydroxypropylmethylcellulose and chitosan coatings with and without bergamot essential oil on quality and safety of cold-stored grapes. Postharvest Biol Technol, 60: 57-63.
33. Pranoto Y, Rakshit SK, Salokhe VM. 2005. Enhancing antimicrobial activity of chitosan films by incorpo-rating garlic oil, potassium sorbate and nisin. LWT Food Sci Technol, 38: 859-865.
34. Sánchez-González L, González-Mart nez C, Chiralt A, Cháfer M. 2010. Physical and antimicrobial properties of chitosan–tea tree essential oil composite films. J Food Eng, 98: 443-452.
35. Sánchez-González L, Cháfer M, Hernández M, Chiralt A, González-Mart nez C. 2011. Antimicrobial activity of polysaccharide films containing essential oils. Food Control, 22: 1302-1310.
36. Zivanovic Z, Chı S, Draughon AF. 2005. Antimicrobial Activity of Chitosan Films Enriched with Essential Oils. J Food Sci, 70 (1): 45-51.
37. Sánchez-González L, Cháfer M, Chiralt A, González-Mart nez C. 2010. Physical properties of edible chitosan films containing bergamot essential oil and their inhibitory action on Penicillium italicum. Carbohyd Polym, 82: 277-283.
38. Cemero¤lu B, Karadeniz F. 2001. Meyve Suyu Üretim Teknolojisi. Gıda Teknolojisi Dergisi Yayın No:25, Ankara, Türkiye, 384p.
39. Muzzarelli RAA, Boudrant J, Meyer D, Manno N, DeMarchis M, Paoletti MG. 2012. Current views on fungal chitin/chitosan, human chitinases, food preservation, glucans, pectins and inulin: A tribute to Henri Braconnot, precursor of the carbohydrate polymers science, on the chitin bicentennial. Carbohyd Polym, 87: 995-1012.
40. Domingues RCC, Junior SBF, Silva RB, Cardoso VL, Reis MHM. 2011. Clarification of passion fruit juice with chitosan: Effects of coagulation process variables and comparison with centrifugation and enzymatic treatments, Process Biochem, 1-30.
41. Rungsardthong V, Wongvuttanakul N, Kongpien N, Chotiwaranon P. 2006. Application of fungal chitosan for clarification of apple juice. Process Biochem, 41, 589-593.
42. Chatterjee S, Chatterjee S, Chatterjee BP, Guha AK. 2004. Clarification of fruit juice with chitosan. Process Biochem, 39: 2229-2232.