Pelin Otu Ekstraktlarının Temel Uçucu Yağ Kompozisyonunun, Antibakteriyel ve Antioksidan Aktivitesinin Belirlenmesi (İngilizce)

Öz

Ülkemizin doğu bölgesinde doğal alarak bulunan Artemisia L. türleri (Artemisia absinthium; pelin otu ve Artemisia austriaca; yavşan otu) bu çalışmanın materyali olarak seçilmiştir. Artemisia türlerinin uçucu yağ asitleri gaz kromotografisi-kütle spektrometeresi ile hidro-distilasyon yöntemi kullanılarak analiz edilmiştir. Artemisia absinthium‘a ait temel uçucu yağ -myrcene (% 44.32) iken Artemisia austriaca (% 43.27)‘ait temel uçucu yağ kamphor olarak tespit edilmiştir. Araştırmada kullanılan bitkilerin uçucu yağlara ait antimikrobiyel aktivitesi disk-difüzyon metodu kullanılarak 6 bakteri ile 2 adet maya kullanılarak gerçekleştirilmiştir. Her iki türün benzer antimikrobiyal aktivite gösterdikleri belirlenmiştir. TEAC yöntemi kullanılarak yapılan antioksidan aktivite ölçümlerinde ise A. absinthium’a ait antioksidan aktivite değeri 3.4±1.4 mM Troloks ve A. austriaca için 4.9±1.2 mM Troloks’dur. Araştırmaya konu olan örnekler ABTS radikaline karşı hafif bir antioksidan aktivite göstermişlerdir.

Anahtar Kelimeler

• Artemisia, antioksidan aktivite, antimikrobiyal aktivite, uçucu yağ

Determining Essential Oil Composition, Antibacterial and Antioxidant Activity of Water Wormwood Extracts (in English)

Öz

Artemisia L. species (Artemisiaabsinthium and Artemisia austriaca) naturally distributed through eastern Turkey were chosen as experimental material in this study. Essential oils of these Artemisia species were isolated by hydrodistillation and analysed by gas chromotography-mass spectrometry. The major components were identified as -myrcene (44.32 %) in Artemisiaabsinthium and camphor in Artemisia austriaca (43.27 %), respectively. Essential oils of the plants were tested for antimicrobial activity the disk diffusion method against 6 bacteria and 2 yeast. The essential oil of A. absinthium and A. austriaca showed similar antimicrobial activities. Artemisia species were also antioxidatively active. Using TEAC method, essential oils showed almost the same patterns of antioxidant activities. While A. absinthium showed 3.4±1.4 mM Trolox activity, A. austriaca has 4.9±1.2 mM Trolox. These samples were found to be slightly good radical scavenging activities against ABTS radicals.

Anahtar Kelimeler

• Artemisia, antioxidant activity, antimicrobial activity, essential oil

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