Yıl 2019, Cilt 44 , Sayı 5, Sayfalar 802 - 809 2019-08-17

SUBSTRAT KONSANTRASYONU VE ÖLÇEK BÜYÜTMENİN POLİGALAKTURONAZ ÜRETİMİNE ETKİSİ
EFFECT OF SUBSTRATE CONCENTRATION AND SCALE UP ON THE POLYGALACTURONASE PRODUCTION

Nihan GOGUS [1] , Canan TARI [2]


Pektinazlar, uzun zaman önceden beri bir çok endüstriyel uygulama için kullanılmıştır. Bu enzimlerin en büyük endüstriyel uygulaması meyve suyu ve şarap üretiminde ekstraksiyon, berraklaştırma ve filtrasyon ayrıca meyve ve sebzelerin maserayonudur. Hücre duvarının enzimatik parçalanması yoluyla etki ederler. Bu çalışmada, substrat konsantrasyonunun ve ölçek büyütmenin PG aktivitesi ve biyokütle üretimi üzerindeki etkilerini araştırmak için daha önce erlenlerde optimize edilmiş medya formülasyonunun kesikli tip 1 L ölçekli seri biyoreaktör sistemi ve 5 L ölçekte kullanılması amaçlanmıştır. Sonuç olarak, 5 L ölçekli biyoreaktör deneylerinde elde edilen ortalama PG aktivitesinin (101,29 U/ml), 1 L ölçekli substrat konsantrasyonu deneyinden elde edilen 40 g/L portakal kabuğu konsantrasyonundaki maksimum PG aktivitesinden (88,55 U/ml) daha yüksek olduğu belirlenmiştir. Ayrıca 60 g/L portakal kabuğu konsantrasyonu hariç substrat konsantrasyonu arttıkçaPG aktivitesi de artmıştır.

Pectinases have been used for many industrial applications since long time ago. The largest industrial application of these enzymes is in fruit juice and wine production for the extraction, filtration and clarification and for the maceration of fruits and vegetables. They work by enzymatic breaking down of the cell wall.In this study it was aimed to use the previously optimized shake flask media formulation in batch mode 1 L scale serial bioreactor system and 5 L scale in order to investigate the effects of substrate concentration and scale on PG activity and biomass production. In conclusion it was observed that average PG activity (101.29 U/ml) obtained in 5L scale bioreactor experiments was higher than the maximum PG activity (88.55 U/ml) at 40 g/L orange peel (OP) concentration obtained in the 1 L scale substrate concentration experiment. Furthermore,PG activity increased with an increase in substrate concentration except for 60 g/L orange peel concentration. 

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Birincil Dil en
Konular Fen
Bölüm Makaleler
Yazarlar

Orcid: 0000-0002-6765-0900
Yazar: Nihan GOGUS (Sorumlu Yazar)
Kurum: DOKUZ EYLÜL ÜNİVERSİTESİ
Ülke: Turkey


Orcid: 0000-0001-7184-2764
Yazar: Canan TARI
Kurum: İZMİR YÜKSEK TEKNOLOJİ ENSTİTÜSÜ
Ülke: Turkey


Tarihler

Yayımlanma Tarihi : 17 Ağustos 2019

Bibtex @araştırma makalesi { gida537988, journal = {Gıda}, issn = {1300-3070}, eissn = {1309-6273}, address = {}, publisher = {Gıda Teknolojisi Derneği}, year = {2019}, volume = {44}, pages = {802 - 809}, doi = {10.15237/gida.GD19054}, title = {EFFECT OF SUBSTRATE CONCENTRATION AND SCALE UP ON THE POLYGALACTURONASE PRODUCTION}, key = {cite}, author = {GOGUS, Nihan and TARI, Canan} }
APA GOGUS, N , TARI, C . (2019). EFFECT OF SUBSTRATE CONCENTRATION AND SCALE UP ON THE POLYGALACTURONASE PRODUCTION. Gıda , 44 (5) , 802-809 . DOI: 10.15237/gida.GD19054
MLA GOGUS, N , TARI, C . "EFFECT OF SUBSTRATE CONCENTRATION AND SCALE UP ON THE POLYGALACTURONASE PRODUCTION". Gıda 44 (2019 ): 802-809 <https://dergipark.org.tr/tr/pub/gida/issue/47909/537988>
Chicago GOGUS, N , TARI, C . "EFFECT OF SUBSTRATE CONCENTRATION AND SCALE UP ON THE POLYGALACTURONASE PRODUCTION". Gıda 44 (2019 ): 802-809
RIS TY - JOUR T1 - EFFECT OF SUBSTRATE CONCENTRATION AND SCALE UP ON THE POLYGALACTURONASE PRODUCTION AU - Nihan GOGUS , Canan TARI Y1 - 2019 PY - 2019 N1 - doi: 10.15237/gida.GD19054 DO - 10.15237/gida.GD19054 T2 - Gıda JF - Journal JO - JOR SP - 802 EP - 809 VL - 44 IS - 5 SN - 1300-3070-1309-6273 M3 - doi: 10.15237/gida.GD19054 UR - https://doi.org/10.15237/gida.GD19054 Y2 - 2019 ER -
EndNote %0 Gıda EFFECT OF SUBSTRATE CONCENTRATION AND SCALE UP ON THE POLYGALACTURONASE PRODUCTION %A Nihan GOGUS , Canan TARI %T EFFECT OF SUBSTRATE CONCENTRATION AND SCALE UP ON THE POLYGALACTURONASE PRODUCTION %D 2019 %J Gıda %P 1300-3070-1309-6273 %V 44 %N 5 %R doi: 10.15237/gida.GD19054 %U 10.15237/gida.GD19054
ISNAD GOGUS, Nihan , TARI, Canan . "EFFECT OF SUBSTRATE CONCENTRATION AND SCALE UP ON THE POLYGALACTURONASE PRODUCTION". Gıda 44 / 5 (Ağustos 2019): 802-809 . https://doi.org/10.15237/gida.GD19054
AMA GOGUS N , TARI C . EFFECT OF SUBSTRATE CONCENTRATION AND SCALE UP ON THE POLYGALACTURONASE PRODUCTION. GIDA. 2019; 44(5): 802-809.
Vancouver GOGUS N , TARI C . EFFECT OF SUBSTRATE CONCENTRATION AND SCALE UP ON THE POLYGALACTURONASE PRODUCTION. Gıda. 2019; 44(5): 809-802.