Effect of Heat Treatment on Chlorophyll Degradation and Color Loss in Green Peas (in English)

Yıl 2008, Cilt: 33 Sayı: 5, 225 - 233, 01.10.2008

Hande Selen Erge Feryal Karadeniz Nuray Koca Yeşim Soyer

Öz

Thermal degradation kinetics of chlorophyll and visual green color loss in green peas were investigated at 70°, 80°, 90° and 100 °C. The degradation of chlorophyll a and chlorophyll b followed a first-order kinetic model. The loss of visual green color, as represented by the change of the -a, the ratio -a/b and h (hue) values measured by tristimulus colorimeter, also followed a first-order reaction. The activation energies for chlorophylls a and b were determined as 47.78 and 26.77 kJ mol-1, while those for -a, -a/b and h values were found as 49.75, 56.04 and 55.06 kJ mol-1, respectively.

Anahtar Kelimeler

Chlorophyll, kinetics, green peas, color

Bezelyelerde Klorofil Degradasyonu ve Renk Kaybı Üzerine Isıl İşlemin Etkisi (İngilizce)

Öz

Bezelyelerde klorofilin termal degradasyon kinetiği ve görünür yeşil renkteki kayıp 70°, 80°, 90° ve 100 °C’de araştırılmıştır. Klorofil a ve klorofil b’nin parçalanması birinci dereceden bir kinetik model izlemektedir. Tristimulus kolorimetresi ile ölçülen -a, -a/b ve h (hue) değerlerindeki değişim ile ortaya konulan görünür yeşil renkteki kayıp da birinci dereceden reaksiyona uymaktadır. Klorofil a ve b için aktivasyon enerjileri sırasıyla 47.78 and 26.77 kJ mol-1olarak belirlenirken; -a, -a/b ve h değerleri için aktivasyon enerjileri 49.75, 56.04 and 55.06 kJ mol-1 olarak saptanmıştır.

Anahtar Kelimeler

Klorofil, kinetik, bezelye, renk

Kaynakça

• Almela L, Fernández-López JA and Roca MJ. 2000. High-performance liquid chromatographic screening of chlorophyll derivatives produced during fruit storage. J. Chromatogr. A, 870: 483-489.
• Steet JA and Tong CH. 1996. Degradation kinetics of green color and chlorophylls in peas by colorimetry and HPLC. J. Food Sci., 61: 924-927.
• Tan CT and Francis FJ. 1962. Effect of processing temperature on pigments and color of spinach. J. Food Sci., 27: 232- 240. 4. Lajollo F, Tannenbaum SR. and Labuza TP. 1971. Reaction at limited water concentrations. Chlorophyll degradation. J. Food Sci., 36: 850-853.
• Schwartz SJ and Von Elbe JH. 1983. Kinetics of chlorophyll degradation to pyropheophytin in vegetables. J. Food Sci., 48: 1303-1306.
• Canjura FL, Schwartz SJ and Nunes RV. 1991. Degradation kinetics of chlorophylls and chlorophyllides. J Food Sci., 56: 1639-1643. 7. Schwartz SJ and Lorenzo TV. 1991. Chlorophyll stability during continuous aseptic processing and storage. J. Food Sci., 56: 1059-1062.
• Tonucci LH and Von Elbe JH. 1992. Kinetics of the formation of zinc complexes of chlorophyll derivatives. J. Agric. Fo- od Chem., 40: 2341-2344.
• Canjura FL and Schwartz SJ. 1991. Separation of chlorophyll compounds and their polar derivatives by high-perfor- mance liquid chromatography. J. Agric. Food Chem., 39: 1102-1105.
• Heaton JW and Marangoni AG. 1996. Chlorophyll degradation in processed foods and senescent plant tissues. Trends Food Sci. Technol., 7: 8-15.
• Minguez-Mosquera MI, Garrido-Fernandez J and Gandul-Rojas B. 1989. Pigment changes in olives during fermentati- on and brine storage. J. Agric. Food Chem., 37: 8-11.
• Mangos TJ and Berger RG. 1997. Determination of major chlorophyll degradation products. Z. Lebensm.Unters. Forsch. A, 204: 345-350.
• Van Boekel MAJS. 1999. Testing of kinetic models: usefulness of the multiresponse approach as applied to chlorophyll degradation in foods. Food Res. Int., 32: 261-269.
• Van Boekel MAJS. 2000. Kinetic modelling in food science: a case study on chlorophyll degradation in olives. J. Sci. Food Agric., 80: 3-9.
• La Borde LF and Von Elbe JH. 1990. Zinc complex formation in heated vegetable purees. J. Agric. Food Chem., 38: 484-487.
• Weemaes C, Ooms V, Van Loey AM and Hendrickx ME. 1999. Kinetics of chlorophyll degradation and color loss in hea- ted broccoli juice. J. Agric. Food Chem., 47: 2404-2409.
• White RC, Jones ID and Gibbs E.1963. Determination of chlorophylls, chlorophylldes,pheophytins, and pheophorbides in plant material. J. Food Sci., 28: 431- 436.
• Von Elbe JH and Schwartz SJ. 1996. Colorants. In: Food Chemistry (Ed. O.R. Fennema), Marcel Dekker, New York, pp.651-722,
• Clydesdale FM and Francis FJ. 1968. Chlorophyll changes in thermally processed spinach as influenced by enzyme conversion and pH adjustment. Food Technol., 22: 793-796.
• Buckle KA and Edwards RA. 1970. Chlorophyll color and pH changes in HTST processed green pea puree. J. Food Technol., 5: 173-178.
• Gnanasekharan V, Shewfelt RL and Chinnan MS. 1992. Detection of color changes in green vegetables. J Food Sci., 57: 149-154.
• Barrett DM, Garcia EL, Russell GF, Ramirez E and Shirazi A. 2000. Blanch time and cultivar effects on quality of fro- zen and stored corn and broccoli. J. Food Sci., 65: 534-540.
• Gunawan, MI and Barringer SA. 2000. Green color degradation of blanched broccoli (Brassica oleracea) due to acid and microbial growth. J. Food Process. Pres., 24: 253-263.
• Tijkens LMM, Barringer SA and Biekman ESA. 2001. Modelling the effect of pH on the colour degradation of blanched broccoli. Innov Food Sci Emerg Technol., 2:315-322.
• Cano, MP and Marin MA 1992. Pigment composition and color of frozen and canned kiwi fruit slices. J. Agric. Food Chem., 40: 2141-2146.
• Labuza, TP and Riboh D. 1982. Theory and application of Arrhenius kinetics to the prediction of nutrients losses in fo- ods. Food Technol., 36: 66-74.
• Labuza, TP. 1984. Application of chemical kinetics to deterioration of foods. J. Chem. Educ., 61: 348-358.
• Hayakawa KI and Timbers GE. 1977. Influence of heat treatment on the quality of vegetables: changes in visual gre- en color. J Food Sci., 42: 778-781.
• Schwartz SJ, Woo SL and Von Elbe JH. 1981. High performance liquid chromatography of chlorophylls and their deri- vatives in fresh and processed spinach. J. Agric. Food Chem., 29: 533-535.
• Gupte SM and Francis FJ. 1964. Effect of pH adjustment high-temperature short-time processing on color and pigment retention in spinach puree. Food Technol., 18: 1645-1658.