Effects of Antimicrobial and Antioxidant Activity of Pomegranate Peel Extract on The Quality of Beef Meatballs

Yıl 2014, Cilt: 39 Sayı: 6, 355 - 362, 01.12.2014

Hazret Özdemir Ayla Soyer Şeref Tağı Mehser Turan

Öz

The antioxidant and antimicrobial effect of pomegranate peel extract (PE) was investigated in meatballs during refrigerated storage at 4±1oC. Lyophilised powder extract obtained from water extract of pomegranate peel was incorporated into freshly minced beef meat at 0.1%, 0.2% and 0.3% concentrations and the results from these incorporations were compared with 0.01% BHT added and control (without any antioxidant) samples. PE showed higher antioxidant activity than BHT. PE at 0.2% and 0.3% levels had antimicrobial activity against only Staphylococcus aureus among the bacteria tested. The growth of total viable microorganisms in meatball was significantly suppressed by PE addition as compared to control and BHT samples. The increase in Enterobacteriaceae counts was reduced by the increase in PE concentration during 6 days of storage. PE treatment (0.1% to 0.3%) reduced lipid oxidation. Addition of 0.2% and 0.3% PE into meatball prevented the perception of off-odors and putrefactive odors for 6 days. As a result, PE can be succesfully used to extend shelf life of meatballs.

Anahtar Kelimeler

Pomegranate peel extract, microbial characteristics, lipid oxidation, meatball, cold storage

Nar Kabuğu Ekstraktının Antimikrobiyel ve Antioksidan Aktivitesinin Köfte Kalitesine Etkisi

Öz

Bu çalışmada, soğukta depolanan köftenin mikrobiyel ve oksidatif stabilitesine nar kabuğu ekstraktı (NKE)’nın etkisi araştırılmıştır. Nar kabuğundan elde edilen sulu ekstrakttan liyofilize toz ekstrakt elde edilmiş ve köfte formülasyonuna %0.1 (NKE1), %0.2 (NKE2) ve %0.3 (NKE3) konsantrasyonlarında ilave edilmiştir. NKE içeren köfteler, %0.01 BHT içeren ve herhangi bir antioksidan kaynağı içermeyen kontrol örneklerle aerobik ortamda 4±1oC’de depolama süresince karşılaştırılmıştır. NKE, BHT’den daha yüksek antioksidan aktivite göstermiştir. Antimikrobiyel aktivite testlerinde, kullanılan NKE konsantrasyonlarından %0.2 ve %0.3 test edilen bakterilerden sadece Staphylococcus aureus üzerine inhibitör etki göstermiştir. Köftede TAMB, TAPB gelişimi, kontrol ve BHT’li örneklerle karşılaştırıldığında NKE ilavesi ile baskılanmıştır. NKE konsantrasyonu arttıkça, Enterobacteriaceae sayısındaki artış daha az olmuştur. Diğer yandan Lipid oksidasyonu, artan NKE düzeyi ile geciktirilmiştir. Köfteye %0.2-%0.3 NKE ilavesi, kötü koku oluşumunu ve kokuşmanın algılanmasını 6 gün süreyle engellemiştir. Sonuç olarak, nar kabuğu, köftelerin raf ömrünü uzatmak amacıyla uygun bir doğal katkı maddesi olma potansiyeline sahiptir.

Anahtar Kelimeler

Nar kabuğu ekstraktı, mikrobiyel özellik, lipid oksidasyonu, köfte, soğuk depolama 

Kaynakça

• Lambert AD, Smith JP, Dodds KL. 1991. Shelf life extension and microbial safety of fresh meat – A Review. Food Microbiol, 8, 267-297.
• Fernández J, Perez-Alvarez JA, Fernández-López JA. 1997. Thiobarbituric acid test for monitoring lipid oxidation in meat. Food Chem, 59, 345-353. 3. Fernández-López J, Zhi N, Aleson-Carbonell L, Pérez-Alvarez, JA, Kuri V. 2005. Antioxidant and antibacterial activities of natural extracts: application in beef meatballs. Meat Sci, 69, 371-380.
• Shan B, Cai YZ, Brooks JD, Corke H. 2009. Antibacterial and antioxidant effects of five spice and herb extracts as natural preservative of raw pork. J Sci Food Agric, 89, 1879-1885.
• Anon. 2013. Spoilage of Food Products-Meat and Meat Products (Chapter 4), Lectures of Ghent University. Department of Food Safety and Food Quality. http://pathogencombat.wur.nl (Eriflim tarihi 01.03.2014).
• Dainty RH, Mackey BM. 1992. The relationship between the phenotypic properties of bacteria from chill-stored meat and spoilage processes. J Appl Bacteriol(Supplement), 73(S), 103-114.
• Varnam AH, Sutherland JP. 1995. Meat and Meat Products. Chapman and Hall, London.
• Decker EA, Xiong YL, Calvert JT, Crum AD, Blanchard SP. 1993. Chemical, physical and functional properties of oxidized turkey white muscle myofibrillar proteins. J Agric Food Chem, 41, 186-189. 9. Shahidi F. 1997. Natural Antioxidants An Overview. In: Natural Antioxidants: Chemistry, Health Effects and Applications, Shaihidi, F (Ed), AOCS Press, Illinois, pp. 1-11.
• Çizelge 1. NKE içeren köftelerin 4±1°C’de 6 gün depolama sonrası duyusal de¤erlendirme sonuçları
• Table Sensory evaluation scores of PE treated raw meatballs after 6 days of storage at 4±1°C Uygulama Treatment Ransit koku Rancid odor Off-odor Putrefactive odor
• General acceptability K BHT NKE1 NKE2 NKE3 5.00±0.00a 4.70±0.14b 5.00±0.00a 4.90±0.14a 5.10±0.14a 5.30±0.07a 5.35±0.07a 5.45±0.21a
• a,b,c: Her bir sütunda farklı harf taflıyan ortalamalar arasındaki fark önemlidir (P<0.01).
• a,b,c: Different letters indicate significant difference (P<0.01) in each column.
• Soyer A, fiahin ME. 1999. Effect of glazing and storage time on lipid oxidation of frozen chub mackerel (Scomber japonicus). Turk J Vet Anim Sci, 23, 575-584.
• Wu JM, Lee MH, Ho CT, Chang SS. 1982. Elucidation of the chemical structures of natural antioxidants isolated from rosemary. J Am Oil Chem So, 59(8), 339-345.
• Lindberg Madson H, Bertelsen G. 1995. Spices as antioxidants. Trends Food Sci Tech, 6, 271-278.
• Balasundram N, Sundram K, Samman S. 2006. Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chem, 99, 191-203.
• Vuorela S, Salminen H, Makeda M, Kivikari R, Karonen M, Heinonen M. 2005. Effect of plant phenolics on protein and lipid oxidation in cooked pork meat patties. J Agric Food Chem, 53, 8492-8497. 15. Wang R, Ding Yi, Liu R, Xiang L, Du L. 2010. Pomegranate: Constituents, bioactivities and pharmacokinetics. Fruit Veg Cereal Sci Biotech, 4(2),77-87.
• Tomas-Barberan F, Espin JC. 2001. Phenolic compounds and related enzymes as determinants of quality of fruit and vegetables. J Sci Food Agric, 81, 853-876.
• Guo, C, Yang J, Wei J, Li Y, Xu J, Jiang Y. 2003. Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutr Res, 23, 1719-1726.
• Wang Z, Pan Z, Ma H, Atungulu GG. 2011. Extract of phenolic from pomegranate peels. The Open Food Science Journal, 5, 17-25.
• Li Y, Gou C, Yang J, Wei J, Xu J, Cheng S. 2006. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chem, 96, 254-260.
• Negi PS, Jayaprakasha GK. 2003. Antioxidant and antibacterial activities of Punica granatum peel extracts. J Food Sci, 68(4), 1473-1477.
• Nuamsetti T, Dechayuenyong P, Tantipaibulvut S. 2012. Antibacterial activity of pomegranate fruit peels and arils. ScienceAsia, 38, 219-322.
• Naveena BM, Sen AR, Kingsly RP, Singh DB, Kondaiah N. 2008. Antioxidant activity of pomegranate rind powder extract in cooked chicken patties. Int J Food Sci Technol, 43, 1807-1812. 23. Kanatt SR, Chander R, Sharma A. 2010. Antioxidant and antimicrobial activity of pomegranate peel extract improves the shelf life of chicken products. Int J Food Sci Technol, 45, 216-222.
• Singleton VL, Rossi JA Jr. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult, 16, 144-158.
• Arts MJTJ, Haenen GRMM, Voss HP, Bast A. 2001. Masking of antioxidant capacity by the interaction of flavonoids with protein. Food Chem Toxicol, 39, 787-791.
• Lenette EH. 1985. Manual of Clinical Microbiology. 3rd ed, American Society for Microbiology.
• Alanis AD, Calzada F, Cervantes JA, Torres J,Ceballos JM. 2005. Antibacterial properties of some plants used in Mexican traditional medicines for the treatment of gastrointestinal disorder. J Ethnopharmacol, 100, 153-157.
• Nasr CB, Ayed N, Metche M. 1996. Quantitative determination of the phenolic content of pomegranate peel. Z LebensmUnters Forsch, 203, 374-378.
• Chidamabaramurty KN, Jayaprakasha GK, Singh RP. 2002. Antioxidant activity of pomegranate peel extracts in vivo models. J Agric Food Chem, 50, 4791-4795.
• Sagdic O, Ozturk I, Yilmaz MT, Yetim H. 2011. Effect of grape pomace extracts obtained from different grape varieties on microbial quality of beef patty. J Food Sci, 76(7), M515-M521.
• ICMSF. 1986. Microorganisms in foods.2. Sampling for microbiological analysis: Principles and specific applications (2nd ed.), Toronto: University of Toronto Press.
• Ahn J, Grün IU, Mustapha A. 2007. Effects of plant extracts on microbial growth, color change, and lipid oxidation in cooked beef. Food Microbiol, 24, 7-14.
• Brown JE, Khodr H, Hider RC, Rica-Evans CA. 1998. Structural dependence of flavonoid interactions with Cu+2ions: implications for their antioxidant properties. Biochem J, 330,1173-1178.