Achillea gypsicola Hub. Mor. In vitro Hücre Kültürlerinde Seçilmiş Ağır Metallerin Hücre Büyümesi ve Kamfor Birikimi Üzerindeki Etkisi

Öz

Farmasötik aktif bileşenlerin bitki dokularında birikimini arttırmak için abiyotik ve biyotik elisitörlerin kullanımı dünya çapında artan bir ilgi kazanmıştır. Bu çalışma, Achillea gypsicola hücre kültüründe kadmiyum klorür (CdCl2) ve gümüş nitrat (AgNO3) kullanılarak kamfor ve fenolik bileşik birikimini teşvik etmeyi amaçlamıştır. Sekiz günlük kültürlerden büyüyen hücreler, kontrol ile birlikte üç konsantrasyonda (5, 25 ve 50 uM) CdCl2 ve AgNO3 ile muamele edildi. Kamfor ve fenolik bileşik miktar tayini sırasıyla Headspace-GC-MS ve spektrofotometre kullanılarak yapıldı. Kamfor ve fenolik bileşik miktarı, hücre sayısı ve hücre kuru ağırlığı, artan AgNO3 ve CdCl2 dozlarından önemli ölçüde etkilenmiştir. Kamfor miktarındaki en önemli değişiklik, sırasıyla 6.88 ve 6.32 kat artışla 25 uM CdCl2 ve AgNO3 ile muamele edilen hücrede gözlendi. Bununla birlikte, 50 uM AgNO3 ve CdCl2'nin uygulanması, çalışılan tüm özelliklerde hızlı bir düşüşle sonuçlandı ve bu, A. gypsicola'nın süspansiyon kültürünün bu elisitörlerin yüksek konsantrasyonları tarafından ortaya çıkmaya duyarlı olduğunu ima etti. Sonuç olarak, A. gypsicola'nın kültürlenmiş dokularında AgNO3 ve CdCl2 elisitörlerinin kullanılması, tıbbi öneme sahip arzu edilen biyoaktif bileşiklerin üretiminin arttırılması için büyük önem taşıyacaktır.

Anahtar Kelimeler

• Compositae
• Elisitör
• Doğal bileşikler
• Terpenoid

Influence of Selected Heavy Metals on Cell Growth and Camphor Secretion in Achillea gypsicola Hub. Mor. In vitro Cell Cultures

Öz

The use of abiotic and biotic elicitors for increasing the accumulation of pharmaceutical active ingredients in plant tissues has gained an increasing interest worldwide. This study was intented to provide promoting accumulation of camphor and phenolic compound using cadmium chloride (CdCl2) and silver nitrate (AgNO3) in cell culture of Achillea gypsicola. Growing cells from 8-day-old cultures were treated with three concentrations (5, 25 and 50 µM) of CdCl2 and AgNO3, along with the control. The quantification of camphor and phenolic compound were performed using Headspace-GC-MS and spectrophotometer, respectively. The content of camphor and phenolic compound, cell number and cell dry weight were significantly affected by increasing doses of AgNO3 and CdCl2. The highest significant change in camphor content was observed in cell treated with 25 µM CdCl2 and AgNO3 with a 6.88 and 6.32 fold increase, respectively. The application of 50 µM AgNO3 and CdCl2, however, resulted in a rapid decine in all attributes studied, implying that culture of A. gypsicola is susceptible to elicitation by high concentrations of these elicitors. In conclusion, using AgNO3 and CdCl2 elicitors in cultured tissues of A. gypsicola would be of great importance to enhanced production of desired bioactive compounds of medicinal importance

Anahtar Kelimeler

• Compositae
• Elicitor
• Natural products
• Terpenoid

Kaynakça

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