Balıktan İzole Edilen Hareketli Aeromonas Türlerinin Bazı Ekstrasellüler Enzimlerinin Belirlenmesi

Fatma Özdemir; Seza Arslan

doi:10.5281/zenodo.4843082

Balıktan İzole Edilen Hareketli Aeromonas Türlerinin Bazı Ekstrasellüler Enzimlerinin Belirlenmesi

Öz

Aeromonas türleri yaygın olarak çevrede özellikle deniz ve taze su kaynaklarında bulunmakta, balık ve deniz ürünlerini sıklıkla kontamine etmektedir. Bu türler insanlarda ve balıklarda enfeksiyon etkeni olan fırsatçı patojenler olarak kabul edilmektedir. İnsanlarda gastrointestinal, ekstraintestinal ve yara infeksiyonlarına neden olur. Bu çalışmada, açık halk pazarı ve marketlerden temin edilen balık örneklerinden elde edilen hareketli Aeromonas türlerinde bazı ekstrasellüler enzim genlerinin varlığının belirlenmesi amaçlanmıştır. Çalışmada 8 A. hydrophila, 19 A. caviae ve 9 A. veronii biovar sobria olmak üzere toplam 36 hareketli Aeromonas izolatında sıcaklık-duyarlı proteaz (eprCAI), serin proteaz (ser), elastaz (ela) ve lipaz (lip) genlerinin varlığı PZR tekniği ile araştırılmıştır. İzolatların 8’i (%22,2) eprCAI, 32’si (%88,9) ser, 23’ü (%63,9) ela ve 17’si (%47,2) lip geni için pozitif bulunmuştur. A. hydrophila izolatlarının hepsinde (%100) eprCAI, ser ve lip enzim genlerinin taşındığı saptanmıştır. A. veronii bv sobria izolatlarının tamamında ser gen bölgesi pozitif bulunmuştur. Ancak bu izolatların hiçbirinin eprCAI ve lip enzim genlerini taşımadığı belirlenmiştir. Bu çalışma sonuçları, balık örneklerinden izole edilen hareketli Aeromonas türlerinde en az bir veya daha fazla ekstrasellüler enzim kodlayan genlerin varlığını göstermiştir. Virulans ve gıdanın bozulması ile ilişkili ekstrasellüler enzimleri salgılayan hareketli Aeromonas izolatlarının gıdada varlığı, gıda hijyeni ve güvenliği açısından sorun oluşturabilir.

Anahtar Kelimeler

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Fatma Özdemir ^*
0000-0002-4804-936X
Türkiye

Seza Arslan
0000-0002-2478-6875
Türkiye

Yayımlanma Tarihi

1 Mayıs 2021

Gönderilme Tarihi

1 Mart 2021

Kabul Tarihi

31 Mart 2021

Yayımlandığı Sayı

Yıl 2021 Cilt: 2 Sayı: 1

DOI

https://doi.org/10.5281/zenodo.4843082

IZ

https://izlik.org/JA24LT97JP

Kaynak Göster

RIS / Bibtex

APA

Özdemir, F., & Arslan, S. (2021). Balıktan İzole Edilen Hareketli Aeromonas Türlerinin Bazı Ekstrasellüler Enzimlerinin Belirlenmesi. Gazi Üniversitesi Fen Fakültesi Dergisi, 2(1), 13-23. https://doi.org/10.5281/zenodo.4843082

AMA

1.Özdemir F, Arslan S. Balıktan İzole Edilen Hareketli Aeromonas Türlerinin Bazı Ekstrasellüler Enzimlerinin Belirlenmesi. GÜFFD. 2021;2(1):13-23. doi:10.5281/zenodo.4843082

Chicago

Özdemir, Fatma, ve Seza Arslan. 2021. “Balıktan İzole Edilen Hareketli Aeromonas Türlerinin Bazı Ekstrasellüler Enzimlerinin Belirlenmesi”. Gazi Üniversitesi Fen Fakültesi Dergisi 2 (1): 13-23. https://doi.org/10.5281/zenodo.4843082.

EndNote

Özdemir F, Arslan S (01 Mayıs 2021) Balıktan İzole Edilen Hareketli Aeromonas Türlerinin Bazı Ekstrasellüler Enzimlerinin Belirlenmesi. Gazi Üniversitesi Fen Fakültesi Dergisi 2 1 13–23.

IEEE

[1]F. Özdemir ve S. Arslan, “Balıktan İzole Edilen Hareketli Aeromonas Türlerinin Bazı Ekstrasellüler Enzimlerinin Belirlenmesi”, GÜFFD, c. 2, sy 1, ss. 13–23, May. 2021, doi: 10.5281/zenodo.4843082.

ISNAD

Özdemir, Fatma - Arslan, Seza. “Balıktan İzole Edilen Hareketli Aeromonas Türlerinin Bazı Ekstrasellüler Enzimlerinin Belirlenmesi”. Gazi Üniversitesi Fen Fakültesi Dergisi 2/1 (01 Mayıs 2021): 13-23. https://doi.org/10.5281/zenodo.4843082.

JAMA

1.Özdemir F, Arslan S. Balıktan İzole Edilen Hareketli Aeromonas Türlerinin Bazı Ekstrasellüler Enzimlerinin Belirlenmesi. GÜFFD. 2021;2:13–23.

MLA

Özdemir, Fatma, ve Seza Arslan. “Balıktan İzole Edilen Hareketli Aeromonas Türlerinin Bazı Ekstrasellüler Enzimlerinin Belirlenmesi”. Gazi Üniversitesi Fen Fakültesi Dergisi, c. 2, sy 1, Mayıs 2021, ss. 13-23, doi:10.5281/zenodo.4843082.

Vancouver

1.Fatma Özdemir, Seza Arslan. Balıktan İzole Edilen Hareketli Aeromonas Türlerinin Bazı Ekstrasellüler Enzimlerinin Belirlenmesi. GÜFFD. 01 Mayıs 2021;2(1):13-2. doi:10.5281/zenodo.4843082